Human ST14/HAI/MTSP1/SNC19/ARCI11/PRSS14/TADG15 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
ST14 (suppressor of tumorigenicity 14 protein) is an epithelial-derived, 1 pass type II transmembrane serine protease belonging to the peptidase S1 family. ST14 contains 2 CUB, 4 LDL-receptor class A, 1 peptidase S1 and 1 SEA domains in the extracellular region. ST14 may form a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. ST14 degrades extracellular matrix and exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. ST14 mat be involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing. The expression of ST14 has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. A mutation in ST14 is associated with autosomal recessive ichthyosis with hypotrichosis.
Gene Symbol: ST14; HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14
NCBI Gene ID: 6768
Uniprot Entry: Q9Y5Y6
Construct Details: Full length human ST14 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 2568 (bp)
Gene Insert Sequence:
ATGGGGAGCGATCGGGCCCGCAAGGGCGGAGGGGGCCCGAAGGACTTCGGCGCGGGACTCAAGTACAACT
CCCGGCACGAGAAAGTGAATGGCTTGGAGGAAGGCGTGGAGTTCCTGCCAGTCAACAACGTCAAGAAGGT
GGAAAAGCATGGCCCGGGGCGCTGGGTGGTGCTGGCAGCCGTGCTGATCGGCCTCCTCTTGGTCTTGCTG
GGGATCGGCTTCCTGGTGTGGCATTTGCAGTACCGGGACGTGCGTGTCCAGAAGGTCTTCAATGGCTACA
TGAGGATCACAAATGAGAATTTTGTGGATGCCTACGAGAACTCCAACTCCACTGAGTTTGTAAGCCTGGC
CAGCAAGGTGAAGGACGCGCTGAAGCTGCTGTACAGCGGAGTCCCATTCCTGGGCCCCTACCACAAGGAG
TCGGCTGTGACGGCCTTCAGCGAGGGCAGCGTCATCGCCTACTACTGGTCTGAGTTCAGCATCCCGCAGC
ACCTGGTGGAGGAGGCCGAGCGCGTCATGGCCGAGGAGCGCGTAGTCATGCTGCCCCCGCGGGCGCGCTC
CCTGAAGTCCTTTGTGGTCACCTCAGTGGTGGCTTTCCCCACGGACTCCAAAACAGTACAGAGGACCCAG
GACAACAGCTGCAGCTTTGGCCTGCACGCCCGCGGTGTGGAGCTGATGCGCTTCACCACGCCCGGCTTCC
CTGACAGCCCCTACCCCGCTCATGCCCGCTGCCAGTGGGCCCTGCGGGGGGACGCCGACTCAGTGCTGAG
CCTCACCTTCCGCAGCTTTGACCTTGCGTCCTGCGACGAGCGCGGCAGCGACCTGGTGACGGTGTACAAC
ACCCTGAGCCCCATGGAGCCCCACGCCCTGGTGCAGTTGTGTGGCACCTACCCTCCCTCCTACAACCTGA
CCTTCCACTCCTCCCAGAACGTCCTGCTCATCACACTGATAACCAACACTGAGCGGCGGCATCCCGGCTT
TGAGGCCACCTTCTTCCAGCTGCCTAGGATGAGCAGCTGTGGAGGCCGCTTACGTAAAGCCCAGGGGACA
TTCAACAGCCCCTACTACCCAGGCCACTACCCACCCAACATTGACTGCACATGGAACATTGAGGTGCCCA
ACAACCAGCATGTGAAGGTGCGCTTCAAATTCTTCTACCTGCTGGAGCCCGGCGTGCCTGCGGGCACCTG
CCCCAAGGACTACGTGGAGATCAACGGGGAGAAATACTGCGGAGAGAGGTCCCAGTTCGTCGTCACCAGC
AACAGCAACAAGATCACAGTTCGCTTCCACTCAGATCAGTCCTACACCGACACCGGCTTCTTAGCTGAAT
ACCTCTCCTACGACTCCAGTGACCCATGCCCGGGGCAGTTCACGTGCCGCACGGGGCGGTGTATCCGGAA
GGAGCTGCGCTGTGATGGCTGGGCCGACTGCACCGACCACAGCGATGAGCTCAACTGCAGTTGCGACGCC
GGCCACCAGTTCACGTGCAAGAACAAGTTCTGCAAGCCCCTCTTCTGGGTCTGCGACAGTGTGAACGACT
GCGGAGACAACAGCGACGAGCAGGGGTGCAGTTGTCCGGCCCAGACCTTCAGGTGTTCCAATGGGAAGTG
CCTCTCGAAAAGCCAGCAGTGCAATGGGAAGGACGACTGTGGGGACGGGTCCGACGAGGCCTCCTGCCCC
AAGGTGAACGTCGTCACTTGTACCAAACACACCTACCGCTGCCTCAATGGGCTCTGCTTGAGCAAGGGCA
ACCCTGAGTGTGACGGGAAGGAGGACTGTAGCGACGGCTCAGATGAGAAGGACTGCGACTGTGGGCTGCG
GTCATTCACGAGACAGGCTCGTGTTGTTGGGGGCACGGATGCGGATGAGGGCGAGTGGCCCTGGCAGGTA
AGCCTGCATGCTCTGGGCCAGGGCCACATCTGCGGTGCTTCCCTCATCTCTCCCAACTGGCTGGTCTCTG
CCGCACACTGCTACATCGATGACAGAGGATTCAGGTACTCAGACCCCACGCAGTGGACGGCCTTCCTGGG
CTTGCACGACCAGAGCCAGCGCAGCGCCCCTGGGGTGCAGGAGCGCAGGCTCAAGCGCATCATCTCCCAC
CCCTTCTTCAATGACTTCACCTTCGACTATGACATCGCGCTGCTGGAGCTGGAGAAACCGGCAGAGTACA
GCTCCATGGTGCGGCCCATCTGCCTGCCGGACGCCTCCCATGTCTTCCCTGCCGGCAAGGCCATCTGGGT
CACGGGCTGGGGACACACCCAGTATGGAGGCACTGGCGCGCTGATCCTGCAAAAGGGTGAGATCCGCGTC
ATCAACCAGACCACCTGCGAGAACCTCCTGCCGCAGCAGATCACGCCGCGCATGATGTGCGTGGGCTTCC
TCAGCGGCGGCGTGGACTCCTGCCAGGGTGATTCCGGGGGACCCCTGTCCAGCGTGGAGGCGGATGGGCG
GATCTTCCAGGCCGGTGTGGTGAGCTGGGGAGACGGCTGCGCTCAGAGGAACAAGCCAGGCGTGTACACA
AGGCTCCCTCTGTTTCGGGACTGGATCAAAGAGAACACTGGGGTATAG
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
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Product datasheet (pdf) and additional supporting documents, including COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.