Human KIT/SCFR/PBT/CD117/C-KIT Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Product ID: KIT-Fc (SKU#: FCL2650)

Price:

$588.00

Description

KIT, also known as c-KIT, SCFR (stem cell factor receptor), PBT (Piebald trait protein), and CD117, is a single pass type I transmembrane protein belonging to the receptor tyrosine kinase (RTK) family and the CSF-1/PDGF receptor subfamily. KIT is a cellular homolog of the feline sarcoma virus protein, v-Kit. Kit contains 5 immunoglobulin (Ig)-like C2-type domains in the extracellular domain (ECD) and 1 split tyrosine kinase domain in the cytoplasmic region. Alternative splicing of KIT generates a potentially secreted isoform corresponding to the first 4 Ig-like domains. KIT may act as cell-surface receptor for the cytokine KITLG/SCF and play an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF, KIT can activate PI3K and AKT1 signaling pathways. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases, MAPK1/ERK2 and/or MAPK3/ERK1. KIT promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Mutations in the KIT gene are associated with gastrointestinal stromal tumors (GIST), mast cell disease, acute myelogenous leukemia (AML), and piebaldism. KIT protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction.

Gene Symbol: KIT; PBT; SCFR; C-Kit; CD117

NCBI Gene ID: 3815

Uniprot Entry: P10721

Construct Details: The recombinant human KIT-Fc protein is expressed as a 737-amino acid protein consisting of Gln26 - Pro524 region of KIT with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.

Source: Human cells stably expressing human KIT-Fc and growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

10 20 30 40 50 60

QPSVSPGEPS PPSIHPGKSD LIVRVGDEIR LLCTDPGFVK WTFEILDETN ENKQNEWITE

70 80 90 100 110 120

KAEATNTGKY TCTNKHGLSN SIYVFVRDPA KLFLVDRSLY GKEDNDTLVR CPLTDPEVTN

130 140 150 160 170 180

YSLKGCQGKP LPKDLRFIPD PKAGIMIKSV KRAYHRLCLH CSVDQEGKSV LSEKFILKVR

190 200 210 220 230 240

PAFKAVPVVS VSKASYLLRE GEEFTVTCTI KDVSSSVYST WKRENSQTKL QEKYNSWHHG

250 260 270 280 290 300

DFNYERQATL TISSARVNDS GVFMCYANNT FGSANVTTTL EVVDKGFINI FPMINTTVFV

310 320 330 340 350 360

NDGENVDLIV EYEAFPKPEH QQWIYMNRTF TDKWEDYPKS ENESNIRYVS ELHLTRLKGT

370 380 390 400 410 420

EGGTYTFLVS NSDVNAAIAF NVYVNTKPEI LTYDRLVNGM LQCVAAGFPE PTIDWYFCPG

430 440 450 460 470 480

TEQRCSASVL PVDVQTLNSS GPPFGKLVVQ SSIDSSAFKH NGTVECKAYN DVGKTSAYFN

490 500 510 520 530 540

FAFKGNNKEQ IHPHTLFTPS TTENLYFQGS TGTHTCPPCP APELLGGPSV FLFPPKPKDT

550 560 570 580 590 600

LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH

610 620 630 640 650 660

QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK NQVSLTCLVK

670 680 690 700 710 720

GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE

730

ALHNHYTQKS LSLSPGK

M.W.: Calculated molecular mass (kDa): 82.8 Estimated by SDS-PAGE under reducing condition (kDa): 90-100.

Calculated PI: 6.27

Calculated extinction coefficients (M-1 cm-1, at 280nm): 106325

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Binds its ligand KITL/SCF (SKU#FCL2346 and #FCL2384) and neutralizes its mediated signaling activity.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. EMBO J. 6:3341-3351(1987)

2. Oncogene 7:2207-2217(1992)

3. J. Biol. Chem. 269:21793-21802(1994)

4. Cell. Mol. Life Sci. 61:2535-2548(2004)

5. Clin. Cancer Res. 14:1926-1930(2008)

Product datasheet (pdf) can be downloaded here: FCL2650-PDS.pdf