Human GRM5/mGlu5/GPRC1E/MGLUR5 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

GRM5 is a 7 transmembrane (7TM) receptor belonging to the family 3 (also known as family C) of the G-protein-coupled receptors (GPCRs). Structurally they are composed of 4 elements; an N-terminal signal peptide; a large hydrophilic extracellular agonist-binding region; a region containing 7TM domains; and a C-terminal cytoplasmic domain of variable length.  Family 3 members include the metabotropic glutamate receptors (mGluRs), the extracellular calcium-sensing receptors (CASRs), the gamma-amino-butyric acid (GABA) type B receptors, and the vomeronasal type-2 receptors.  These GPCRs regulate many important physiological processes and many of them are promising targets for drug development. mGluRs are distinct from the ionotropic glutamate receptors and are coupled to G-proteins to stimulate the inositol phosphate/Ca2+ signaling pathway. At least 8 subtypes of mGluR (MGR1-8) have been identified and they differ in their agonist pharmacology and signal transduction pathways.  They can be divided into 3 groups: Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C; Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.  GRM5 may play an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.

 

 

Gene Symbol: mGlu5; GPRC1E; MGLUR5; PPP1R86

 

NCBI Gene ID: 2915

 

Uniprot Entry: P41594

 

Construct Details: Full length human GRM5 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3639 (bp)

 

Gene Insert Sequence:  

ATGGTCCTTCTGTTGATCCTGTCAGTCTTACTTTTGAAAGAAGATGTCCGTGGGAGTGCACAGTCCAGTG

AGAGGAGGGTGGTGGCTCACATGCCGGGTGACATCATTATTGGAGCTCTCTTTTCTGTTCATCACCAGCC

TACTGTGGACAAAGTTCATGAGAGGAAGTGTGGGGCGGTCCGTGAACAGTATGGCATTCAGAGAGTGGAG

GCCATGCTGCATACCCTGGAAAGGATCAATTCAGACCCCACACTCTTGCCCAACATCACACTGGGCTGTG

AGATAAGGGACTCCTGCTGGCATTCGGCTGTGGCCCTAGAGCAGAGCATTGAGTTCATAAGAGATTCCCT

CATTTCTTCAGAAGAGGAAGAAGGCTTGGTACGCTGTGTGGATGGCTCCTCCTCTTCCTTCCGCTCCAAG

AAGCCCATAGTAGGGGTCATTGGGCCTGGCTCCAGTTCTGTAGCCATTCAGGTCCAGAATTTGCTCCAGC

TTTTCAACATACCTCAGATTGCTTACTCAGCAACCAGCATGGATCTGAGTGACAAGACTCTGTTCAAATA

TTTCATGAGGGTTGTGCCTTCAGATGCTCAGCAGGCAAGGGCCATGGTGGACATAGTGAAGAGGTACAAC

TGGACCTATGTATCAGCCGTGCACACAGAAGGCAACTATGGAGAAAGTGGGATGGAAGCCTTCAAAGATA

TGTCAGCGAAGGAAGGGATTTGCATCGCCCACTCTTACAAAATCTACAGTAATGCAGGGGAGCAGAGCTT

TGATAAGCTGCTGAAGAAGCTCACAAGTCACTTGCCCAAGGCCCGGGTGGTGGCCTGCTTCTGTGAGGGC

ATGACGGTGAGAGGTCTGCTGATGGCCATGAGGCGCCTGGGTCTAGCGGGAGAATTTCTGCTTCTGGGCA

GTGATGGCTGGGCTGACAGGTATGATGTGACAGATGGATATCAGCGAGAAGCTGTTGGTGGCATCACAAT

CAAGCTCCAATCTCCCGATGTCAAGTGGTTTGATGATTATTATCTGAAGCTCCGGCCAGAAACAAACCAC

CGAAACCCTTGGTTTCAAGAATTTTGGCAGCATCGTTTTCAGTGCCGACTGGAAGGGTTTCCACAGGAGA

ACAGCAAATACAACAAGACTTGCAATAGTTCTCTGACTCTGAAAACACATCATGTTCAGGATTCCAAAAT

GGGATTTGTGATCAACGCCATCTATTCGATGGCCTATGGGCTCCACAACATGCAGATGTCCCTCTGCCCA

GGCTATGCAGGACTCTGTGATGCCATGAAGCCAATTGATGGACGGAAACTTTTGGAGTCCCTGATGAAAA

CCAATTTTACTGGGGTTTCTGGAGATACGATCCTATTCGATGAGAATGGAGACTCTCCAGGAAGGTATGA

AATAATGAATTTCAAGGAAATGGGAAAAGATTACTTTGATTATATCAACGTTGGAAGTTGGGACAATGGA

GAATTAAAAATGGATGATGATGAAGTATGGTCCAAGAAAAGCAACATCATCAGATCTGTGTGCAGTGAAC

CATGTGAGAAAGGCCAGATCAAGGTGATCCGAAAGGGAGAAGTCAGCTGTTGTTGGACCTGTACACCTTG

TAAGGAGAATGAGTATGTCTTTGATGAGTACACATGCAAGGCATGCCAACTGGGGTCTTGGCCCACTGAT

GATCTCACAGGTTGTGACTTGATCCCAGTACAGTATCTTCGATGGGGTGACCCTGAACCCATTGCAGCTG

TGGTGTTTGCCTGCCTTGGCCTCCTGGCCACCCTGTTTGTTACTGTAGTCTTCATCATTTACCGTGATAC

ACCAGTAGTCAAGTCCTCAAGCAGGGAACTCTGCTACATTATCCTTGCTGGCATCTGCCTGGGCTACTTA

TGTACCTTCTGCCTCATTGCGAAGCCCAAACAGATTTACTGCTACCTTCAGAGAATTGGCATTGGTCTCT

CCCCAGCCATGAGCTACTCAGCCCTTGTAACAAAGACCAACCGTATTGCAAGGATCCTGGCTGGCAGCAA

GAAGAAGATCTGTACCAAAAAGCCCAGATTCATGAGTGCCTGTGCCCAGCTAGTGATTGCTTTCATTCTC

ATATGCATCCAGTTGGGCATCATCGTTGCCCTCTTTATAATGGAGCCTCCTGACATAATGCATGACTACC

CAAGCATTCGAGAAGTCTACCTGATCTGTAACACCACCAACCTAGGAGTTGTCACTCCACTTGGATACAA

TGGATTGTTGATTTTGAGCTGCACCTTCTATGCGTTCAAGACCAGAAATGTTCCAGCTAACTTCAACGAG

GCCAAGTATATCGCCTTCACAATGTACACGACCTGCATTATATGGCTAGCTTTTGTGCCAATCTACTTTG

GCAGCAACTACAAAATCATCACCATGTGTTTCTCGGTCAGCCTCAGTGCCACAGTGGCCCTAGGCTGCAT

GTTTGTGCCGAAGGTGTACATCATCCTGGCCAAACCAGAGAGAAACGTGCGCAGCGCCTTCACCACATCT

ACCGTGGTGCGCATGCATGTAGGGGATGGCAAGTCATCCTCCGCAGCCAGCAGATCCAGCAGCCTAGTCA

ACCTGTGGAAGAGAAGGGGCTCCTCTGGGGAAACCTTAAGGTACAAAGACAGGAGACTGGCCCAGCACAA

GTCGGAAATAGAGTGTTTCACCCCCAAAGGGAGTATGGGGAATGGTGGGAGAGCAACAATGAGCAGTTCC

AATGGAAAATCCGTCACGTGGGCCCAGAATGAGAAGAGCAGCCGGGGGCAGCACCTGTGGCAGCGCCTGT

CCATCCACATCAACAAGAAAGAAAACCCCAACCAAACGGCCGTCATCAAGCCCTTCCCCAAGAGCACGGA

GAGCCGTGGCCTGGGCGCTGGCGCTGGCGCAGGCGGGAGCGCTGGGGGCGTGGGGGCCACGGGCGGTGCG

GGCTGCGCAGGCGCCGGCCCAGGCGGGCCCGAGTCCCCAGACGCCGGCCCCAAGGCGCTGTATGATGTGG

CCGAGGCTGAGGAGCACTTCCCGGCGCCCGCGCGGCCGCGCTCACCGTCGCCCATCAGCACGCTGAGCCA

CCGCGCGGGCTCGGCCAGCCGCACGGACGACGATGTGCCGTCGCTGCACTCGGAGCCTGTGGCGCGCAGC

AGCTCCTCGCAGGGCTCCCTCATGGAGCAGATCAGCAGTGTGGTCACCCGCTTCACGGCCAACATCAGCG

AGCTCAACTCCATGATGCTGTCCACCGCGGCCCCCAGCCCCGGCGTCGGCGCCCCGCTCTGCTCGTCCTA

CCTGATCCCCAAAGAGATCCAGTTGCCCACGACCATGACGACCTTTGCCGAAATCCAGCCTCTGCCGGCC

ATCGAAGTCACGGGAGGCGCGCAGCCCGCGGCAGGGGCGCAGGCGGCTGGGGACGCGGCCCGGGAGAGCC

CCGCGGCCGGTCCCGAGGCTGCGGCCGCCAAGCCAGACCTGGAGGAGCTGGTGGCTCTCACCCCGCCGTC

CCCCTTCAGAGACTCGGTGGACTCGGGGAGCACAACCCCCAACTCGCCAGTGTCCGAGTCGGCCCTCTGT

ATCCCGTCGTCTCCCAAATATGACACTCTTATCATAAGAGATTACACTCAGAGCTCCTCGTCGTTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

 

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.