Human LPHN3/ADGRL3/CL3/LEC3/CIRL3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
LPHN3 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. LPHN3 may be proteolytically processed to form a heterodimer of 2 chains: the large extracellular N-terminal fragment and the membrane-bound C-terminal 7TM fragment predominantly remain associated and non-covalently linked. LPHN3 may play a role in cell-cell adhesion and neuron guidance via its interactions with FLRT2 and FLRT3 that are expressed at the surface of adjacent cells. It may also have a role in the development of glutamatergic synapses in the cortex. LPHN3 is important in determining the connectivity rates between the principal neurons in the cortex.
Gene Symbol: LPHN3; ADGRL3; CL3; LEC3; CIRL3; LPHN-3; CIRL-3; LEC-3; KIAA0768
NCBI Gene ID: 23284
Uniprot Entry: Q9HAR2
Construct Details: Full length human LPHN3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 4410 (bp)
Gene Insert Sequence:
ATGTGGCCATCGCAGCTACTAATTTTCATGATGCTCTTAGCTCCAATAATTCATGCTTTCAGCCGTGCCC
CAATTCCAATGGCTGTGGTCCGCAGAGAGCTATCCTGTGAGAGCTATCCTATAGAGCTTCGCTGTCCAGG
AACAGACGTCATCATGATAGAAAGTGCCAACTATGGCAGGACTGATGACAAAATTTGTGACTCTGACCCT
GCTCAGATGGAGAATATCCGATGTTATCTGCCAGATGCCTATAAGATTATGTCTCAAAGATGCAATAACA
GAACCCAGTGTGCAGTGGTGGCAGGTCCTGATGTTTTTCCAGACCCGTGTCCAGGAACCTATAAATACCT
TGAAGTGCAGTATGAATGTGTCCCTTACAAAGTGGAACAAAAAGTTTTTCTTTGTCCTGGACTACTAAAA
GGAGTATACCAGAGTGAACATTTGTTTGAGTCCGACCACCAATCTGGGGCGTGGTGCAAAGACCCTCTGC
AGGCATCTGACAAGATTTATTATATGCCCTGGACTCCCTACAGAACTGATACCCTGACTGAGTATTCATC
CAAGGATGACTTCATTGCTGGAAGACCAACTACAACCTACAAGCTCCCTCACAGGGTGGATGGCACAGGA
TTTGTAGTGTATGATGGAGCTTTGTTCTTCAACAAAGAGCGCACCAGGAACATAGTAAAGTTTGATTTGC
GGACTAGGATAAAGAGTGGAGAGGCTATCATAGCAAATGCCAATTACCATGATACCTCCCCTTACCGATG
GGGAGGCAAATCTGACATAGACCTGGCAGTAGATGAGAATGGGCTATGGGTAATCTATGCAACAGAACAA
AACAATGGTAAAATTGTCATTAGTCAATTGAACCCTTACACCCTACGGATCGAAGGAACATGGGATACTG
CATATGATAAAAGGTCAGCTTCCAATGCCTTTATGATTTGTGGAATTCTGTATGTGGTCAAATCTGTATA
TGAGGATGATGACAATGAGGCTACTGGAAATAAGATTGACTACATTTACAACACTGACCAAAGCAAGGAT
AGTTTGGTGGATGTACCCTTTCCTAATTCATACCAGTACATTGCAGCTGTGGATTACAACCCCAGGGACA
ACCTACTTTATGTATGGAATAACTATCACGTCGTGAAATATTCTTTGGATTTTGGACCTCTGGATAGTAG
ATCAGGGCAGGCACATCATGGACAAGTTTCATACATTTCTCCGCCAATTCACCTTGACTCTGAGCTAGAA
AGACCCTCTGTTAAAGATATCTCTACCACAGGACCTCTTGGCATGGGAAGCACTACCACCAGTACCACCC
TTCGGACCACAACTTTGAGCCCAGGAAGGAGTACCACCCCGTCAGTGTCAGGAAGAAGAAACCGGAGTAC
TAGTACCCCATCTCCAGCTGTCGAGGTACTTGATGACATGACCACACACCTTCCATCAGCATCGTCCCAA
ATCCCAGCTCTCGAAGAGAGCTGTGAGGCTGTGGAAGCCCGAGAAATCATGTGGTTTAAGACTCGTCAAG
GACAGATAGCAAAGCAGCCATGCCCTGCAGGAACTATAGGTGTATCAACTTATCTATGCCTTGCTCCTGA
TGGAATTTGGGATCCCCAAGGTCCAGATCTCAGCAACTGTTCTTCTCCTTGGGTCAATCATATAACACAG
AAGTTGAAATCTGGTGAAACAGCTGCCAACATTGCTAGAGAGCTGGCTGAACAGACAAGAAATCACTTGA
ATGCTGGGGACATCACCTACTCTGTCCGGGCCATGGACCAGCTGGTAGGCCTCCTAGATGTACAGCTTCG
GAACTTGACCCCAGGTGGAAAAGATAGTGCTGCCCGGAGTTTGAACAAGCTTCAGAAAAGAGAGCGCTCT
TGCAGAGCCTATGTCCAGGCAATGGTCGAGACAGTTAACAACCTCCTTCAGCCACAAGCTTTGAATGCAT
GGAGAGACCTGACTACGAGTGATCAGCTGCGTGCGGCCACCATGTTGCTTCATACTGTGGAGGAAAGTGC
TTTTGTGCTGGCTGATAACCTTTTGAAGACTGACATTGTCAGGGAGAATACAGACAATATTAAATTGGAA
GTTGCAAGACTGAGCACAGAAGGAAACTTAGAAGACCTAAAATTTCCAGAAAACATGGGCCATGGAAGCA
CTATCCAGCTGTCTGCAAATACCTTAAAGCAAAATGGCCGAAATGGAGAGATCAGAGTGGCCTTTGTCCT
GTATAACAACTTGGGTCCTTATTTATCCACGGAGAATGCCAGTATGAAGTTGGGAACGGAAGCTTTGTCC
ACAAATCATTCTGTTATTGTCAATTCCCCTGTTATTACGGCAGCAATAAACAAAGAGTTCAGTAACAAGG
TTTATTTGGCTGATCCTGTGGTATTTACTGTTAAACATATCAAGCAGTCAGAGGAAAATTTCAACCCTAA
CTGTTCATTTTGGAGCTACTCCAAGCGTACAATGACAGGTTATTGGTCAACACAAGGCTGTCGGCTCCTG
ACAACAAATAAGACACATACTACATGCTCTTGTAACCACCTAACAAATTTTGCAGTACTGATGGCACATG
TGGAAGTTAAGCACAGTGATGCGGTCCATGACCTCCTTCTGGATGTGATCACGTGGGTTGGAATTTTGCT
GTCCCTTGTTTGTCTCCTGATTTGCATCTTCACATTTTGCTTTTTCCGGGGGCTCCAGAGTGACCGTAAC
ACCATCCACAAGAACCTCTGCATCAGTCTCTTTGTAGCAGAGCTGCTCTTCCTGATTGGGATCAACCGAA
CTGACCAACCAATTGCCTGTGCTGTTTTCGCTGCCCTGTTACATTTCTTCTTCTTGGCTGCCTTCACCTG
GATGTTCCTGGAGGGGGTGCAGCTTTATATCATGCTGGTGGAGGTTTTTGAGAGTGAACATTCACGTAGG
AAATACTTTTATCTGGTCGGCTATGGGATGCCTGCACTCATTGTGGCTGTGTCAGCTGCAGTAGACTACA
GGAGTTATGGAACAGATAAAGTATGTTGGCTCCGACTTGACACCTACTTCATTTGGAGTTTTATAGGACC
AGCAACTTTGATAATTATGCTTAATGTAATCTTCCTTGGGATTGCTTTATATAAAATGTTTCATCATACT
GCTATACTGAAACCTGAATCAGGCTGTCTTGATAACATCAACTATGAGGATAACAGACCCTTCATCAAGT
CATGGGTTATAGGTGCAATAGCTCTTCTCTGCCTATTAGGATTGACCTGGGCCTTTGGACTCATGTATAT
TAATGAAAGCACAGTCATCATGGCCTATCTCTTCACCATTTTCAATTCTCTACAGGGAATGTTTATATTT
ATTTTCCATTGTGTCCTACAGAAGAAGGTACGAAAAGAGTATGGGAAATGCCTGCGAACACATTGCTGTA
GTGGCAAAAGTACAGAGAGTTCCATTGGTTCAGGGAAAACATCTGGTTCTCGAACTCCTGGACGCTACTC
CACAGGCTCACAGAGCCGAATCCGTAGAATGTGGAATGACACGGTTCGAAAGCAGTCAGAGTCTTCCTTT
ATTACTGGAGACATAAACAGTTCAGCGTCACTCAACAGAGAGGGGCTTCTGAACAATGCCAGGGATACAA
GTGTCATGGATACTCTACCACTGAATGGTAACCATGGCAATAGTTACAGCATTGCCAGCGGCGAATACCT
GAGCAACTGTGTGCAAATCATAGACCGTGGCTATAACCATAACGAGACCGCCCTAGAGAAAAAGATTCTG
AAGGAACTCACTTCCAACTATATCCCTTCTTACCTGAACAACCATGAGCGCTCCAGTGAACAGAACAGGA
ATCTGATGAACAAGCTGGTGAATAACCTTGGCAGTGGAAGGGAAGATGATGCCATTGTCCTGGATGATGC
CACCTCGTTTAACCACGAGGAGAGTTTGGGCCTGGAACTCATTCATGAGGAATCTGATGCTCCTTTGCTG
CCCCCAAGAGTATACTCCACCGAGAACCACCAGCCACACCATTATACCAGAAGGCGGATCCCCCAAGACC
ACAGTGAGAGCTTTTTCCCTTTGCTAACCAACGAGCACACAGAAGATCTCCAGTCACCCCATAGAGACTC
TCTCTATACCAGCATGCCGACACTGGCTGGTGTGGCCGCCACAGAGAGTGTTACCACCAGCACCCAGACC
GAACCCCCACCGGCCAAATGTGGTGATGCCGAAGATGTTTACTACAAAAGCATGCCAAACCTAGGCTCCA
GAAACCACGTCCATCAGCTGCATACTTACTACCAGCTAGGTCGCGGCAGCAGTGATGGATTTATAGTTCC
TCCAAACAAAGATGGGACCCCTCCCGAGGGAAGTTCAAAAGGACCGGCTCATTTGGTCACTAGTCTATAG
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Ann Rev Biochem 56: 615 (1987)
2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.