Human GPR126/ADGRG6/APG1/DREG/VIGR Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
GPR126 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. GPR126 is an Orphan receptor and may be required for normal differentiation of promyelinating Schwann cells and for normal myelination of axons. GRP126 is upregulated in human umbilical vein endothelial cells. Variations in the GPR126 gene may affect a person's stature.
Gene Symbol: GPR126; ADGRG6; APG1; DREG; VIGR; PS1TP2
NCBI Gene ID: 57211
Uniprot Entry: Q86SQ4
Construct Details: Full length human GPR126 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 3666 (bp)
Gene Insert Sequence:
ATGATGTTTCGCTCAGATCGAATGTGGAGCTGCCATTGGAAATGGAAGCCCAGTCCTCTCCTGTTCTTAT
TTGCTTTATATATCATGTGTGTTCCTCACTCAGTGTGGGGATGTGCCAACTGCCGAGTGGTTTTGTCCAA
CCCTTCTGGGACCTTTACTTCTCCATGCTACCCTAACGACTACCCAAACAGCCAGGCTTGCATGTGGACG
CTCCGAGCCCCCACCGGTTATATCATTCAGATAACATTTAACGACTTTGACATTGAAGAAGCTCCCAATT
GCATTTATGACTCATTATCCCTTGATAATGGAGAGAGCCAGACTAAATTTTGTGGAGCAACTGCCAAAGG
CCTATCATTTAACTCAAGTGCGAATGAGATGCATGTGTCCTTTTCAAGTGACTTTAGCATCCAGAAGAAA
GGTTTCAATGCCAGCTACATCAGAGTTGCCGTGTCCTTAAGGAATCAAAAGGTCATTTTACCCCAGACAT
CAGATGCTTACCAGGTATCTGTTGCAAAAAGCATCTCTATTCCAGAGCTCAGTGCTTTCACACTCTGCTT
TGAAGCAACCAAAGTTGGCCATGAAGACAGTGATTGGACAGCTTTCTCCTACTCAAATGCATCCTTCACA
CAATTGCTCAGTTTTGGAAAGGCCAAGAGTGGCTACTTTCTATCCATTTCTGATTCAAAATGTTTGTTGA
ATAATGCATTACCTGTCAAAGAAAAAGAAGACATTTTTGCAGAAAGCTTTGAACAGCTCTGCCTTGTTTG
GAATAATTCTTTGGGCTCTATTGGTGTAAATTTCAAAAGAAACTATGAAACAGTTCCATGTGATTCTACC
ATTAGTAAAGTTATTCCTGGGAATGGGAAATTGTTGTTGGGCTCCAATCAAAATGAAATTGTCTCTCTAA
AAGGGGACATTTATAACTTTCGACTTTGGAATTTTACCATGAATGCCAAAATCCTCTCCAACCTCAGCTG
TAATGTGAAAGGGAATGTAGTCGACTGGCAAAATGACTTCTGGAATATCCCAAACCTAGCTCTGAAAGCT
GAAAGCAACCTAAGCTGTGGTTCCTACCTGATCCCGCTCCCAGCAGCAGAACTGGCCAGCTGTGCAGACC
TGGGGACCCTCTGTCAAGCTACTGTAAACTCTCCTAGTACTACACCACCCACTGTCACCACTAACATGCC
TGTTACTAACAGAATCGATAAACAAAGGAATGATGGAATTATCTATAGAATATCCGTAGTGATTCAGAAC
ATCCTTCGTCACCCTGAGGTAAAAGTACAGAGCAAGGTGGCAGAATGGCTCAATTCAACCTTCCAAAATT
GGAACTACACGGTTTATGTCGTTAATATCAGTTTTCACCTGAGTGCTGGAGAGGACAAGATTAAAGTCAA
GAGAAGCCTTGAGGATGAGCCAAGGTTGGTGCTTTGGGCCCTTCTAGTTTACAATGCTACCAACAATACT
AATTTGGAAGGAAAAATCATTCAGCAGAAGCTCCTAAAAAATAATGAGTCCTTGGATGAAGGCTTGAGGC
TACATACAGTGAATGTGAGACAACTGGGTCATTGTCTTGCCATGGAGGAACCCAAAGGCTACTACTGGCC
ATCTATCCAACCTTCTGAATACGTTCTTCCTTGTCCAGACAAGCCTGGCTTTTCTGCTTCTCGGATATGT
TTTTACAATGCTACCAACCCATTGGTAACCTACTGGGGACCTGTTGATATCTCCAACTGTTTAAAAGAAG
CAAATGAAGTTGCTAACCAGATTTTAAATTTAACTGCTGATGGGCAGAACTTAACCTCAGCCAATATTAC
CAACATTGTGGAACAGGTCAAAAGAATTGTGAATAAAGAAGAAAACATTGATATAACACTTGGCTCAACT
CTAATGAATATATTTTCTAATATCTTAAGCAGTTCAGACAGTGACTTGCTTGAGTCATCTTCTGAAGCTT
TAAAAACAATTGATGAATTGGCCTTCAAGATAGACCTAAATAGCACATCACATGTGAATATTACAACTCG
GAACTTGGCTCTCAGCGTATCATCCCTGTTACCAGGGACAAATGCAATTTCAAATTTTAGCATTGGTCTT
CCAAGCAATAATGAATCGTATTTCCAGATGGATTTTGAGAGTGGACAAGTGGATCCACTGGCATCTGTAA
TTTTGCCTCCAAACTTACTTGAGAATTTAAGTCCAGAAGATTCTGTATTAGTTAGAAGAGCACAGTTTAC
TTTCTTCAACAAAACTGGACTTTTCCAGGATGTAGGACCCCAAAGAAAAACTTTAGTGAGTTATGTGATG
GCGTGCAGTATTGGAAACATTACTATCCAGAATCTGAAGGATCCTGTTCAAATAAAAATCAAACATACAA
GAACTCAGGAAGTGCATCATCCCATCTGTGCCTTCTGGGATCTGAACAAAAACAAAAGTTTTGGAGGATG
GAACACGTCAGGATGTGTTGCACACAGAGATTCAGATGCAAGTGAGACAGTCTGCCTGTGTAACCACTTC
ACACACTTTGGAGTTCTGATGGACCTTCCAAGAAGTGCCTCACAGTTAGATGCAAGAAACACTAAAGTCC
TCACTTTCATCAGCTATATTGGGTGTGGAATATCTGCTATTTTTTCAGCAGCAACTCTCCTGACATATGT
TGCTTTTGAGAAATTGCGAAGGGATTATCCCTCCAAAATCTTGATGAACCTGAGCACAGCCCTGCTGTTC
CTGAATCTCCTCTTCCTCCTAGATGGCTGGATCACCTCCTTCAATGTGGATGGACTTTGCATTGCTGTTG
CAGTCCTGTTGCATTTCTTCCTTCTGGCAACCTTTACCTGGATGGGGCTAGAAGCAATTCACATGTACAT
TGCTCTAGTTAAAGTATTTAACACTTACATTCGCCGATACATTCTAAAATTCTGCATCATTGGCTGGGGT
TTGCCTGCCTTAGTGGTGTCAGTTGTTCTAGCGAGCAGAAACAACAATGAAGTCTATGGAAAAGAAAGTT
ATGGGAAAGAAAAAGGTGATGAATTCTGTTGGATTCAAGATCCAGTCATATTTTATGTGACCTGTGCTGG
GTATTTTGGAGTCATGTTTTTTCTGAACATTGCCATGTTCATTGTGGTAATGGTGCAGATCTGTGGGAGG
AATGGCAAGAGAAGCAACCGGACCCTGAGAGAAGAAGTGTTAAGGAACCTGCGCAGTGTGGTTAGCTTGA
CCTTTCTGTTGGGCATGACATGGGGTTTTGCATTCTTTGCCTGGGGACCCTTAAATATCCCCTTCATGTA
CCTCTTCTCCATCTTCAATTCATTACAAGGCTTATTTATATTCATCTTCCACTGTGCTATGAAGGAGAAT
GTTCAGAAACAGTGGCGGCAGCATCTCTGCTGTGGTAGATTTCGGTTAGCAGATAACTCAGATTGGAGTA
AGACAGCTACCAATATCATCAAGAAAAGTTCTGATAATCTAGGAAAATCTTTGTCTTCAAGCTCCATTGG
TTCCAACTCAACCTATCTTACATCCAAATCTAAATCCAGCTCTACCACCTATTTCAAAAGGAATAGCCAC
ACAGATAATGTCTCCTATGAGCATTCCTTCAACAAAAGTGGATCACTCAGACAGTGCTTCCATGGACAAG
TCCTTGTCAAAACTGGCCCATGCTGA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Ann Rev Biochem 56: 615 (1987)
2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.