Human GPR124/ADGRA2/TEM5 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
GPR124 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. GPR124 is an endothelial receptor that acts as an essential regulator of CNS angiogenesis. It is required for normal endothelial cell sprouting and migration in the forebrain and neural tube
Gene Symbol: GPR124; ADGRA2; TEM5; KIAA1531
NCBI Gene ID: 25960
Uniprot Entry: Q96PE1
Construct Details: Full length human GPR124 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 4017 (bp)
Gene Insert Sequence:
ATGGGCGCCGGGGGACGCAGGATGCGGGGGGCGCCCGCGCGCCTGCTGCTGCCGCTGCTGCCGTGGCTCC
TGCTGCTCCTGGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCCATCCGCAGCTGCAAGTGCTC
GGGGGAGCGGCCCAAGGGGCTGAGCGGCGGCGTCCCTGGCCCGGCTCGGCGGAGGGTGGTGTGCAGCGGC
GGGGACCTCCCGGAGCCTCCCGAGCCCGGCCTTCTGCCTAACGGCACCGTTACCCTGCTCTTGAGCAATA
ACAAGATCACGGGGCTCCGCAATGGCTCCTTCCTGGGACTGTCACTGCTGGAGAAGCTGGACCTGAGGAA
CAACATCATCAGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCC
AACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATAT
CTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTT
GGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTG
CAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCAGGCCCTGGGCAGCCTCCAGG
AGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGT
GGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGC
TGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCC
ACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGA
GTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCC
TCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCA
TCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCAC
CCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACC
AACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGG
CTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGC
TCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATG
GCCAGCAACCTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCC
GCATCGTGGGTGCCCTGGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAA
TGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCC
TTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGC
CCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTC
CTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCG
GCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCT
TCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCC
CGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGG
CACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCT
GGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGG
CAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGGGAGGTGGGGGGCGCCGGGGCAGGGCTGCAC
CCCGTGGTATACCCCTGCACGGCCTTGCTGCTGCTCTGCCTCTTCGCCACCATCATCACCTACATCCTCA
ACCACAGCTCCATCCGTGTGTCCCGGAAAGGCTGGCACATGCTGCTGAACTTGTGCTTCCACATAGCCAT
GACCTCTGCTGTCTTTGCGGGGGGCATCACACTCACCAACTACCAGATGGTCTGCCAGGCGGTGGGCATC
ACCCTGCACTACTCCTCCCTATCCACGCTGCTCTGGATGGGCGTGAAGGCGCGAGTGCTCCATAAGGAGC
TCACCTGGAGGGCACCCCCTCCGCAAGAAGGGGACCCCGCTCTGCCTACTCCCAGTCCTATGCTCCGGTT
CTATTTGATCGCTGGAGGGATTCCACTCATTATCTGTGGCATCACAGCTGCAGTCAACATCCACAACTAC
CGGGACCACAGCCCCTACTGCTGGCTGGTGTGGCGTCCAAGCCTTGGCGCCTTCTACATCCCTGTGGCTT
TGATTCTGCTCATCACCTGGATCTATTTCCTGTGCGCCGGGCTACGCTTACGGGGTCCTCTGGCACAGAA
CCCCAAGGCGGGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGG
GGCAGCGGCCCCCTCCTGAGTGACTCAGGTTCCCTTCTTGCTACTGGGAGCGCGCGAGTGGGGACGCCCG
GGCCCCCGGAGGATGGTGACAGCCTCTATTCTCCGGGAGTCCAGCTAGGGGCGCTGGTGACCACGCACTT
CCTGTACTTGGCCATGTGGGCCTGCGGGGCTCTGGCAGTGTCCCAGCGCTGGCTGCCCCGGGTGGTGTGC
AGCTGCTTGTACGGGGTGGCAGCCTCCGCCCTGGGCCTCTTCGTCTTCACTCACCACTGTGCCAGGCGGA
GGGACGTGAGAGCCTCGTGGCGCGCCTGCTGCCCCCCTGCCTCTCCCGCGGCCCCCCATGCCCCGCCCCG
GGCCCTGCCCGCCGCCGCAGAGGACGGTTCCCCGGTGTTCGGGGAGGGCCCCCCCTCCCTCAAGTCCTCC
CCAAGCGGCAGCAGCGGCCATCCGCTGGCTCTGGGCCCCTGCAAGCTCACCAACCTGCAGCTGGCCCAGA
GTCAGGTGTGCGAGGCGGGGGCGGCGGCCGGCGGGGAAGGAGAGCCGGAGCCGGCGGGCACCCGGGGAAA
CCTCGCCCACCGCCACCCCAACAACGTGCACCACGGGCGTCGGGCGCACAAGAGCCGGGCCAAGGGACAC
CGCGCGGGGGAGGCCTGCGGCAAGAACCGGCTCAAGGCCCTGCGCGGGGGCGCGGCGGGGGCGCTGGAGC
TGCTGTCCAGCGAGAGCGGCAGTCTGCACAACAGCCCCACCGACAGCTACCTGGGCAGCAGCCGCAACAG
CCCGGGCGCCGGCCTGCAGCTGGAAGGCGAGCCCATGCTCACGCCGTCCGAGGGCAGCGACACCAGCGCC
GCGCCGCTTTCTGAGGCGGGCCGGGCAGGCCAGCGCCGCAGCGCCAGCCGCGACAGTCTCAAGGGCGGCG
GCGCGCTGGAGAAGGAGAGCCATCGCCGCTCGTACCCGCTCAACGCCGCCAGCCTAAACGGCGCCCCCAA
GGGGGGCAAGTACGACGACGTCACCCTGATGGGCGCGGAGGTAGCCAGCGGCGGCTGCATGAAGACCGGA
CTCTGGAAGAGCGAAACTACCGTCTAA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Ann Rev Biochem 56: 615 (1987)
2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.