Human BCAM/CD239 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CD239, also known as BCAM (basal cell adhesion molecule) and Lu (Lutheran blood group glycoprotein), is a single pass type I transmembrane proteins of the Ig superfamily (IgSF). There are two alternatively spliced variants of CD239, which similar structures except for the cytoplasmic tails (one lacking a 40 amino acid SH3 motif). A polymorphism at position 77 within the extracellular domain (ECD) is the basis for the difference between the Lua and Lub Lutheran blood groups.  CD239 contains 5 Ig-like (2 V-type and 3 C2-type) motifs in the extracellular domain (ECD).  CD239 is widely expressed in epithelial and endothelial tissues. It is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. CD239 shares a significant sequence similarity with the CD146/MUC18 and CD166/ALCAM. CD239 cooperates with Integrins β1 and αVβ3 as an adhesion receptor for Laminins. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation.  CD239 is upregulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas. In addition CD239 interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group.

 

 

Gene Symbol: CD239; BCAM; AU; LU; MSK19

 

NCBI Gene ID: 4059

 

Uniprot Entry: P50895

 

Construct Details: Full length human CD239/BCAM gene (encoding UniProt#P50895) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1887 (bp)

 

Gene Insert Sequence:  

ATGGAGCCCCCGGACGCACCGGCCCAGGCGCGCGGGGCCCCGCGGCTGCTGTTGCTCGCAGTCCTGCTGG

CGGCGCACCCAGATGCCCAGGCGGAGGTGCGCTTGTCTGTACCCCCGCTGGTGGAGGTGATGCGAGGAAA

GTCTGTCATTCTGGACTGCACCCCTACGGGAACCCACGACCATTATATGCTGGAATGGTTCCTTACCGAC

CGCTCGGGAGCTCGCCCCCGCCTAGCCTCGGCTGAGATGCAGGGCTCTGAGCTCCAGGTCACAATGCACG

ACACCCGGGGCCGCAGTCCCCCATACCAGCTGGACTCCCAGGGGCGCCTGGTGCTGGCTGAGGCCCAGGT

GGGCGACGAGCGAGACTACGTGTGCGTGGTGAGGGCAGGGGCGGCAGGCACTGCTGAGGCCACTGCGCGG

CTCAACGTGTTTGCAAAGCCAGAGGCCACTGAGGTCTCCCCCAACAAAGGGACACTGTCTGTGATGGAGG

ACTCTGCCCAGGAGATCGCCACCTGCAACAGCCGGAACGGGAACCCGGCCCCCAAGATCACGTGGTATCG

CAACGGGCAGCGCCTGGAGGTGCCCGTAGAGATGAACCCAGAGGGCTACATGACCAGCCGCACGGTCCGG

GAGGCCTCGGGCCTGCTCTCCCTCACCAGCACCCTCTACCTGCGGCTCCGCAAGGATGACCGAGACGCCA

GCTTCCACTGCGCCGCCCACTACAGCCTGCCCGAGGGCCGCCACGGCCGCCTGGACAGCCCCACCTTCCA

CCTCACCCTGCACTATCCCACGGAGCACGTGCAGTTCTGGGTGGGCAGCCCGTCCACCCCAGCAGGCTGG

GTACGCGAGGGTGACACTGTCCAGCTGCTCTGCCGGGGGGACGGCAGCCCCAGCCCGGAGTATACGCTTT

TCCGCCTTCAGGATGAGCAGGAGGAAGTGCTGAATGTGAATCTCGAGGGGAACTTGACCCTGGAGGGAGT

GACCCGGGGCCAGAGCGGGACCTATGGCTGCAGAGTGGAGGATTACGACGCGGCAGATGACGTGCAGCTC

TCCAAGACGCTGGAGCTGCGCGTGGCCTATCTGGACCCCCTGGAGCTCAGCGAGGGGAAGGTGCTTTCCT

TACCTCTAAACAGCAGTGCAGTCGTGAACTGCTCCGTGCACGGCCTGCCCACCCCTGCCCTACGCTGGAC

CAAGGACTCCACTCCCCTGGGCGATGGCCCCATGCTGTCGCTCAGTTCTATCACCTTCGATTCCAATGGC

ACCTACGTATGTGAGGCCTCCCTGCCCACAGTCCCGGTCCTCAGCCGCACCCAGAACTTCACGCTGCTGG

TCCAAGGCTCGCCAGAGCTAAAGACAGCGGAAATAGAGCCCAAGGCAGATGGCAGCTGGAGGGAAGGAGA

CGAAGTCACACTCATCTGCTCTGCCCGCGGCCATCCAGACCCCAAACTCAGCTGGAGCCAATTGGGGGGC

AGCCCCGCAGAGCCAATCCCCGGACGGCAGGGTTGGGTGAGCAGCTCTCTGACCCTGAAAGTGACCAGCG

CCCTGAGCCGCGATGGCATCTCCTGTGAAGCCTCCAACCCCCACGGGAACAAGCGCCATGTCTTCCACTT

CGGCACCGTGAGCCCCCAGACCTCCCAGGCTGGAGTGGCCGTCATGGCCGTGGCCGTCAGCGTGGGCCTC

CTGCTCCTCGTCGTTGCTGTCTTCTACTGCGTGAGACGCAAAGGGGGCCCCTGCTGCCGCCAGCGGCGGG

AGAAGGGGGCTCCGCCGCCAGGGGAGCCAGGGCTGAGCCACTCGGGGTCGGAGCAACCAGAGCAGACCGG

CCTTCTCATGGGAGGTGCCTCCGGAGGAGCCAGGGGTGGCAGCGGGGGCTTCGGAGACGAGTGCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Cancer. Res. 54: 5761 (1994)

2. Proc. Natl., Acad. Sci. 92: 5496 (1995)

3. Blood 88:1865 (1996)

4. Blood 89:4608 (1997).

5. J. Biol. Chem. 274:31903 (1999)

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTP2505-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.