Human CD3e-CD3g Heterodimeric Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant

Product ID: CD3e:CD3g-Fc-Biot (SKU#: FCL655B)

Price:

$578.00

Description

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3e, T3e, TCRe and IMD18, is a single-pass type I transmembrane protein. CD3e contains 1 Ig-like (immunoglobulin-like) domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3 gamma chain, also known as CD3g, T3g and IMD17, is a single-pass type I transmembrane protein. CD3g contains 1 Ig-like domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3e:CD3g is part of the T-cell receptor (TCR)/CD3 complex and is involved in T-cell development and signal transduction. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the invariant subunits of CD3 gamma, delta, epsilon, zeta, and eta. The TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways leading to the activation of T cells. CD3e plays an essential role in T-cell development. In CD3e-deficient mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated. Mutations in the CD3e gene are associated with immunodeficiency 18 (IMD18), an autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood. Defects in the CD3g gene are a cause of immunodeficiency 17 (IMD17), an autosomal recessive primary immunodeficiency characterized by highly variable clinical severity.

Gene Symbol: CD3E; T3E; TCRE; IMD18; Leu-4; CD3G; T3G; IMD17; CD3-GAMMA

NCBI Gene ID: 916; 917

Uniprot Entry: P07766; P09693

Construct Details: The recombinant human CD3e-CD3g heterodimer is co-expressed as 2 chains: (1) a 326-amino acid CD3e-Fc chain consisting of Gln22 – Val118 region of CD3e and a C-terminal Fc from human IgG1; (2) a 327-amino acid CD3g-Fc fusion consisting of Gln23 - Met103 region of CD3g and a C- terminal Fc from human IgG1. It exists as a dimer of heterodimer under non-reducing condition.

Source: Human cells stably expressing CD3e-Fc:CD3g-Fc growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

Chain #1: CD3e-Fc

10 20 30 40 50 60

QDGNEEMGGI TQTPYKVSIS GTTVILTCPQ YPGSEILWQH NDKNIGGDED DKNIGSDEDH

70 80 90 100 110 120

LSLKEFSELE QSGYYVCYPR GSKPEDANFY LYLRARVSGD KTHTCPPCPA PELLGGPSVF

130 140 150 160 170 180

LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR

190 200 210 220 230 240

VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN

250 260 270 280 290 300

QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN

310 320

VFSCSVMHEA LHNHYTQKSL SLSPGK

Chain #1: CD3g-Fc

10 20 30 40 50 60

QSIKGNHLVK VYDYQEDGSV LLTCDAEAKN ITWFKDGKMI GFLTEDKKKW NLGSNAKDPR

70 80 90 100 110 120

GMYQCKGSQN KSKPLQVYYR MSGDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP

130 140 150 160 170 180

EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK

190 200 210 220 230 240

EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI

250 260 270 280 290 300

AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT

310 320

QKSLSLSPGK GGGLNDIFEA QKIEWHE

M.W.: Calculated molecular mass (kDa): 37; Estimated by SDS-PAGE under reducing condition (kDa): ~45

Calculated PI: 8.19

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 52425

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Recognized by recombinant anti-CD3e monoclonal antibodies: human IgG1 (SKU#MAB0818), mouse IgG1 (SKU#MAB1899), mouse IgG2a (SKU#MAB1898) and rabbit IgG (SKU#MAB1897) and other OKT3 derived antibodies that binds CD3e in the context of a CD3e:CD3g complex.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. Nature 321:431 (1986).

2. Nat. Genet. 3:77 (1993).

3. J. Clin. Invest. 114:1512 (2004).

4. Proc. Natl. Acad. Sci. 101:7675 (2004).

5. J Immunol. 183: 1055 (2009).

Product datasheet (pdf) can be downloaded here: FCL655B-PDS.pdf