BAI2 or AGRB2 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways.AGRB2 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions.  AGRB2 may be a brain-specific angiogenesis inhibitor (also known as BAI2). The mature peptide may be further cleaved into additional products. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis. 

BAI2 or AGRB2 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways.AGRB2 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions.  AGRB2 may be a brain-specific angiogenesis inhibitor (also known as BAI2). The mature peptide may be further cleaved into additional products. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis. 

 

Product Details

 

Gene Symbol: AGRB3; ADGRB3; BAI3; KIAA0550

 

NCBI Gene ID: 577

 

Uniprot Entry: O60242

 

Construct Details: Full length human BAI3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4569 (bp)

 

Gene Insert Sequence:  

ATGAAGGCTGTTCGTAACCTGCTGATTTATATATTTTCCACCTATCTCCTGGTTATGTTTGGATTTAATG

CTGCCCAAGACTTCTGGTGTTCAACTTTGGTGAAGGGAGTCATTTATGGATCGTATTCTGTAAGTGAAAT

GTTTCCTAAAAACTTTACAAACTGCACTTGGACGCTGGAAAATCCAGATCCAACCAAATATAGCATTTAC

CTGAAATTTTCCAAAAAGGACCTTAGCTGCTCTAACTTTTCACTCCTGGCTTATCAGTTTGATCATTTTT

CCCATGAAAAAATAAAGGATCTTTTAAGAAAGAATCATTCTATAATGCAACTCTGCAATTCCAAGAATGC

TTTCGTTTTTCTACAGTATGATAAAAATTTTATTCAAATACGTCGAGTATTTCCAACTAATTTCCCAGGA

TTACAGAAAAAAGGGGAAGAAGATCAGAAATCTTTTTTTGAGTTTTTGGTATTGAACAAGGTCAGCCCAA

GCCAGTTTGGTTGCCATGTATTATGTACTTGGTTGGAGAGCTGCTTAAAATCAGAAAATGGGAGAACAGA

ATCATGTGGGATCATGTATACAAAATGCACCTGCCCTCAGCATTTGGGAGAGTGGGGGATCGACGACCAG

TCGCTGATTTTGTTAAATAACGTGGTGTTACCCCTGAATGAGCAGACAGAGGGCTGCCTGACCCAGGAGC

TGCAAACCACCCAAGTCTGCAATCTTACCAGGGAGGCCAAGCGACCACCCAAAGAAGAATTTGGAATGAT

GGGAGATCATACAATTAAAAGTCAGCGACCTCGATCTGTTCATGAAAAAAGGGTCCCTCAGGAACAAGCT

GATGCTGCTAAATTTATGGCACAAACTGGTGAATCTGGTGTGGAAGAGTGGTCCCAGTGGAGCACATGTT

CGGTTACTTGTGGTCAAGGGTCGCAGGTGCGAACCAGAACTTGTGTATCACCTTACGGGACACACTGCAG

CGGCCCATTAAGAGAATCAAGGGTTTGCAATAACACTGCCCTCTGTCCAGTACACGGAGTATGGGAGGAA

TGGTCACCATGGAGTTTATGTTCATTTACATGTGGTCGAGGCCAAAGAACAAGAACAAGGTCATGCACAC

CTCCTCAGTATGGAGGAAGGCCGTGTGAAGGACCTGAAACACATCATAAGCCTTGTAATATTGCTCTTTG

CCCAGTTGATGGACAGTGGCAAGAGTGGAGTTCGTGGAGCCAGTGCTCAGTAACGTGCTCGAATGGGACT

CAGCAGAGAAGCCGGCAGTGCACTGCAGCTGCCCATGGAGGCTCCGAATGCAGAGGGCCATGGGCAGAAA

GCAGAGAGTGCTATAACCCTGAATGTACAGCCAATGGTCAATGGAATCAGTGGGGTCATTGGAGTGGTTG

TTCCAAGTCCTGTGATGGCGGCTGGGAAAGGCGAATAAGGACCTGTCAGGGTGCAGTGATAACAGGGCAG

CAATGTGAAGGAACGGGCGAAGAAGTGAGAAGATGCAATGAGCAGCGATGCCCTGCACCTTATGAAATAT

GCCCTGAGGATTATCTGATGTCGATGGTGTGGAAAAGAACTCCAGCAGGCGACTTGGCATTCAATCAATG

TCCCCTGAATGCCACAGGCACCACTAGCAGACGCTGCTCTCTCAGTCTTCATGGAGTGGCCTTCTGGGAA

CAGCCGAGCTTTGCAAGATGCATATCAAATGAGTACAGACACTTGCAGCATTCAATTAAAGAGCACCTTG

CTAAGGGGCAGCGAATGCTGGCAGGTGATGGAATGTCCCAGGTGACCAAGACACTGTTGGATTTAACTCA

GAGAAAAAATTTCTATGCAGGCGATCTTCTGATGTCTGTGGAGATCCTGAGAAATGTGACAGACACATTT

AAAAGGGCAAGTTACATCCCTGCATCTGATGGTGTCCAGAACTTCTTTCAAATAGTTAGCAACCTTCTAG

ATGAAGAAAACAAGGAAAAATGGGAAGATGCACAACAGATTTATCCAGGGTCAATAGAGTTAATGCAGGT

GATTGAAGATTTTATACACATTGTTGGAATGGGGATGATGGACTTTCAGAATTCATACTTAATGACTGGA

AATGTAGTGGCTAGTATTCAGAAGCTTCCTGCAGCCTCTGTTCTAACAGACATCAACTTTCCAATGAAAG

GACGGAAGGGAATGGTTGACTGGGCAAGAAACTCAGAAGATAGGGTAGTAATTCCAAAAAGCATTTTCAC

TCCGGTGTCATCAAAAGAATTAGATGAATCATCTGTATTTGTTCTTGGCGCAGTCCTATACAAAAACTTA

GATCTAATTTTGCCCACTTTGAGAAATTATACTGTCATTAATTCCAAAATCATCGTGGTCACAATAAGGC

CTGAACCCAAAACAACCGATTCGTTTCTGGAGATAGAACTAGCTCATTTGGCTAATGGTACTTTGAATCC

CTATTGTGTATTGTGGGATGACTCCAAAACGAACGAGTCTTTGGGAACGTGGTCCACCCAGGGATGTAAA

ACTGTGCTTACCGATGCATCCCATACGAAATGCTTATGTGATCGTCTCTCTACCTTCGCCATTTTGGCTC

AGCAACCTAGAGAAATAATCATGGAATCCTCTGGCACACCTTCAGTTACCCTAATAGTAGGCAGTGGTCT

TTCTTGCTTGGCCTTGATTACCCTAGCAGTTGTCTATGCAGCATTATGGAGGTACATACGCTCTGAGAGA

TCCATAATACTAATTAACTTCTGCCTGTCTATCATCTCATCCAATATCCTCATACTGGTTGGACAGACTC

AGACACATAATAAGAGTATCTGCACAACCACCACTGCATTTTTGCACTTTTTCTTCCTGGCTTCATTCTG

TTGGGTTTTGACTGAGGCGTGGCAATCATATATGGCTGTAACTGGAAAAATTAGGACACGGCTTATAAGA

AAACGCTTTTTGTGCCTTGGATGGGGTTTACCAGCATTAGTAGTGGCCACATCAGTAGGCTTCACCAGAA

CAAAAGGATATGGCACTGATCACTACTGCTGGCTCTCTCTTGAAGGAGGACTACTCTATGCTTTTGTGGG

ACCTGCAGCCGCTGTTGTCCTGGTCAACATGGTGATTGGCATTTTGGTATTTAATAAACTTGTTTCCAGA

GATGGAATCCTAGATAAAAAGCTCAAACACAGAGCCGGTCAGATGAGTGAGCCTCATAGCGGTTTGACGC

TCAAATGTGCCAAGTGTGGAGTAGTTTCAACAACAGCTTTGTCAGCCACCACCGCCAGTAACGCCATGGC

GTCTCTTTGGAGCTCCTGTGTGGTGTTGCCCCTTCTGGCTTTGACGTGGATGTCTGCGGTTCTGGCCATG

ACAGATAAACGCTCCATATTGTTTCAAATACTTTTTGCTGTGTTTGATTCATTGCAAGGCTTTGTTATAG

TCATGGTCCACTGCATTCTTCGGAGAGAGGTTCAGGATGCATTTAGATGCCGATTGAGAAACTGTCAGGA

TCCCATCAATGCAGATTCTTCGAGTTCGTTTCCTAATGGGCATGCTCAAATCATGACAGACTTTGAAAAG

GATGTAGACATTGCCTGTCGATCAGTTCTTCATAAGGATATTGGTCCTTGCCGAGCAGCCACAATAACAG

GAACACTTTCTAGGATTTCTCTAAATGATGATGAAGAAGAAAAGGGAACAAACCCTGAAGGGCTAAGCTA

TTCAACATTGCCTGGAAATGTCATTTCCAAAGTCATCATCCAGCAACCCACAGGTTTGCACATGCCCATG

AGTATGAATGAGCTTAGCAATCCATGTTTGAAAAAAGAAAATAGTGAATTGCGGAGAACTGTGTACTTAT

GTACGGATGATAATTTGAGAGGGGCTGACATGGACATAGTCCATCCTCAAGAAAGAATGATGGAAAGTGA

CTATATTGTGATGCCCAGAAGTTCTGTAAATAACCAGCCTTCAATGAAAGAAGAAAGCAAAATGAATATT

GGCATGGAAACCTTGCCGCATGAAAGGCTATTGCACTACAAAGTAAACCCTGAATTCAATATGAATCCCC

CTGTAATGGACCAGTTCAATATGAACTTAGAGCAACATCTCGCACCCCAGGAACATATGCAGAATTTGCC

CTTTGAACCTCGCACAGCTGTGAAGAATTTCATGGCCTCTGAGTTGGATGATAATGCAGGACTATCAAGA

AGTGAAACTGGATCAACGATATCAATGAGTTCTTTAGAGAGAAGAAAATCACGATATTCAGACCTTGACT

TTGAGAAGGTCATGCATACAAGGAAGAGGCATATGGAACTATTTCAAGAACTAAATCAGAAATTTCAAAC

TTTGGACAGATTTCGGGATATACCAAATACAAGCAGTATGGAAAACCCCGCACCAAACAAGAATCCATGG

GACACTTTCAAAAACCCCAGTGAATACCCGCATTACACCACAATCAATGTCTTAGACACAGAGGCAAAGG

ATGCTTTGGAACTGAGGCCAGCAGAGTGGGAGAAGTGTCTGAATTTGCCTCTGGATGTGCAAGAGGGTGA

CTTTCAAACAGAAGTTTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.