BAI2 or AGRB2 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways.AGRB2 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions.  AGRB2 may be a brain-specific angiogenesis inhibitor (also known as BAI2). The mature peptide may be further cleaved into additional products. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis. 

BAI2 or AGRB2 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways.AGRB2 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions.  AGRB2 may be a brain-specific angiogenesis inhibitor (also known as BAI2). The mature peptide may be further cleaved into additional products. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis. 

 

Product Details

 

Gene Symbol: AGRB2; ADGRB2; BAI2

 

NCBI Gene ID: 576

 

Uniprot Entry: O60241

 

Construct Details: Full length human BAI2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4755 (bp)

 

Gene Insert Sequence:  

ATGGAGAATACAGGTTGGATGGGCAAGGGACATAGGATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGA

TTCTGTCCCTGCGCCTGGCCACCGCCTTCGACCCCGCCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGTGT

GCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCCTACCATCGCCTCGGGCTGCTCCTGGACCCTGGAG

AACCCTGACCCCACCAAGTACTCCCTCTACCTGCGCTTCAACCGCCAGGAGCAGGTGTGCGCACACTTTG

CCCCCCGCCTGCTGCCCCTGGACCACTACCTGGTCAACTTTACCTGCCTGCGGCCTAGCCCCGAGGAGGC

GGTGGCCCAGGCGGAGTCAGAGGTGGGGCGGCCAGAAGAGGAGGAGGCAGAGGCGGCAGCGGGGTTGGAG

CTGTGCAGCGGCTCAGGCCCCTTTACCTTCCTGCACTTCGACAAGAACTTCGTGCAGCTGTGCCTGTCGG

CTGAGCCCTCCGAGGCCCCGCGCCTGCTGGCGCCCGCTGCCCTAGCCTTCCGCTTTGTCGAGGTCTTGCT

CATCAACAACAACAACTCTAGCCAATTCACCTGTGGTGTGCTCTGCCGCTGGAGTGAGGAGTGTGGCCGC

GCTGCCGGCAGGGCCTGCGGCTTTGCTCAGCCAGGCTGCAGCTGCCCTGGAGAGGCGGGGGCCGGCTCCA

CCACCACCACATCTCCAGGCCCTCCTGCTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGC

CCCACCTGCTGAGGCCGATTTGCACTCGGGGAGCAGCAATGATCTGTTCACAACCGAGATGAGATATGGT

GAGGAGCCGGAAGAGGAACCGAAAGTGAAAACCCAGTGGCCGAGGTCTGCAGATGAGCCTGGGCTATACA

TGGCGCAGACAGGCGACCCGGCGGCTGAGGAGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCA

GGGTCTGCAGGTGCGGACCCGCTCCTGTGTGTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTGCGG

GAGACCAGGCCCTGCAACAATTCAGCCACCTGCCCAGTGCACGGCGTGTGGGAGGAGTGGGGGTCCTGGA

GCCTGTGCTCCCGCAGCTGCGGGCGGGGGTCCCGGAGCCGGATGCGGACCTGCGTGCCCCCCCAGCACGG

CGGCAAGGCCTGCGAGGGTCCTGAGCTGCAGACTAAGCTCTGCAGTATGGCTGCCTGCCCGGTGGAAGGC

CAGTGGTTAGAATGGGGTCCCTGGGGCCCATGCTCCACGTCCTGTGCCAATGGGACCCAACAGCGCAGCC

GGAAGTGCAGCGTGGCGGGCCCAGCCTGGGCCACATGCACGGGTGCCCTCACTGACACCCGGGAGTGCAG

CAACCTCGAGTGCCCGGCCACTGATAGCAAGTGGGGGCCATGGAATGCGTGGAGCCTGTGCTCTAAGACG

TGTGACACAGGCTGGCAGCGCCGCTTCCGCATGTGCCAGGCCACGGGCACGCAGGGCTACCCCTGCGAGG

GCACCGGAGAGGAGGTGAAGCCTTGTAGTGAGAAGAGGTGTCCAGCCTTCCATGAGATGTGCAGGGATGA

GTACGTGATGCTGATGACGTGGAAGAAGGCAGCTGCTGGCGAGATCATCTACAACAAGTGCCCCCCGAAT

GCCTCAGGGTCTGCCAGCCGCCGCTGTCTCCTCAGTGCCCAAGGCGTGGCGTACTGGGGGCTGCCCAGCT

TTGCTCGCTGCATCTCCCATGAGTACCGCTACCTGTATCTGTCACTTAGGGAGCACCTGGCCAAGGGGCA

GCGCATGCTGGCAGGCGAGGGCATGTCGCAGGTGGTGCGCAGCCTGCAGGAGCTACTGGCCCGGCGCACC

TACTATAGTGGGGACCTGCTCTTCTCTGTGGACATTCTGAGGAATGTCACTGACACCTTTAAGAGGGCCA

CCTACGTGCCCTCGGCTGATGATGTGCAGCGCTTCTTCCAGGTGGTGAGCTTCATGGTGGATGCGGAAAA

CAAGGAGAAGTGGGACGATGCTCAGCAGGTGTCCCCTGGCTCTGTGCACCTGCTCCGTGTCGTGGAGGAC

TTCATTCACCTGGTGGGCGATGCTCTCAAGGCCTTCCAGAGCTCTCTGATTGTCACAGATAATCTAGTGA

TCAGCATTCAGCGAGAGCCCGTCTCAGCTGTGTCCAGTGACATCACGTTCCCCATGCGGGGCCGCCGGGG

CATGAAGGACTGGGTGCGGCACTCAGAGGACCGCCTCTTCCTGCCCAAGGAGGTGCTCAGCCTCTCCTCC

CCAGGGAAGCCAGCCACATCTGGGGCAGCAGGCAGCCCTGGCAGGGGGAGGGGCCCAGGAACGGTGCCTC

CTGGCCCAGGCCACTCCCACCAGCGCCTCCTCCCAGCAGACCCTGATGAGTCCTCCTACTTTGTGATCGG

TGCTGTACTCTACCGCACCCTTGGCCTCATCCTGCCGCCTCCCAGGCCCCCGCTGGCCGTCACATCCCGG

GTGATGACAGTGACTGTGCGCCCCCCTACCCAGCCTCCAGCTGAGCCCCTCATCACTGTGGAGCTCTCCT

ACATCATCAATGGGACCACGGATCCCCATTGCGCCAGCTGGGACTACTCCAGAGCAGATGCCAGCTCAGG

AGACTGGGACACTGAAAATTGCCAGACCCTGGAGACCCAGGCAGCTCACACCCGCTGCCAGTGCCAGCAC

CTGTCCACCTTTGCTGTACTAGCCCAGCCGCCCAAGGACCTGACCCTGGAGCTGGCGGGCTCCCCCTCGG

TCCCCCTGGTGATCGGCTGTGCAGTGTCGTGCATGGCGCTGCTCACCCTGCTCGCCATCTATGCCGCCTT

TTGGAGGTTCATAAAATCTGAACGCTCCATCATCTTGCTGAACTTCTGCCTGTCCATCTTGGCATCCAAC

ATCCTGATCCTCGTGGGCCAGTCCCGGGTGCTGAGCAAGGGCGTGTGCACCATGACGGCTGCCTTCCTGC

ACTTCTTCTTTCTCTCCTCCTTTTGCTGGGTGCTTACCGAGGCCTGGCAGTCCTACCTGGCTGTCATTGG

GCGGATGCGCACCCGCCTCGTTCGCAAGCGCTTCCTCTGCCTGGGCTGGGGTCTGCCTGCCCTGGTGGTG

GCCGTGTCTGTTGGCTTTACCCGAACGAAAGGATACGGTACATCCAGCTACTGCTGGCTCTCCCTGGAGG

GCGGCCTGCTCTACGCCTTTGTGGGCCCTGCAGCCGTCATTGTCCTGGTGAACATGCTCATCGGAATCAT

CGTCTTCAACAAGCTCATGGCACGTGATGGCATCTCCGACAAATCCAAGAAGCAGAGGGCCGGGTCGGAG

CGGTGCCCCTGGGCCAGCCTGCTCCTCCCCTGCTCAGCGTGTGGAGCGGTCCCCAGCCCCCTGCTCAGCT

CAGCCTCGGCCAGGAACGCCATGGCCTCACTCTGGAGCTCCTGCGTGGTGCTGCCCCTGCTGGCGCTCAC

CTGGATGTCTGCCGTCCTGGCTATGACAGACCGCCGTTCCGTCCTCTTCCAGGCCCTCTTTGCTGTCTTC

AACTCCGCGCAGGGCTTTGTCATCACTGCTGTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTGA

AGTGCCAGATGGGGGTGTGCCGGGCTGATGAGAGCGAAGACTCCCCTGACTCGTGTAAGAACGGGCAGCT

GCAGATCCTGTCAGACTTTGAAAAGGATGTGGATCTGGCTTGTCAAACAGTGCTGTTCAAGGAGGTCAAC

ACTTGCAACCCGTCCACCATCACGGGCACACTATCCCGCCTGTCCCTGGATGAGGATGAGGAGCCCAAGT

CCTGCCTCGTGGGCCCTGAGGGCAGCCTCAGCTTCTCACCACTGCCTGGGAATATCCTGGTGCCCATGGC

AGCCTCACCAGGGCTGGGGGAGCCTCCGCCCCCACAGGAGGCCAACCCTGTTTACATGTGTGGGGAGGGT

GGCCTGCGGCAGCTGGACCTCACATGGCTGCGGCCCACTGAGCCAGGCTCTGAGGGAGACTACATGGTGC

TGCCCCGGCGGACTTTGAGCCTGCAGCCTGGCGGTGGGGGTGGAGGTGGTGAGGATGCCCCCAGGGCCCG

GCCGGAGGGGACCCCCCGGCGAGCTGCCAAGACAGTGGCCCACACTGAAGGCTACCCCAGCTTCCTGTCC

GTGGACCACTCGGGCCTGGGGCTGGGCCCTGCCTATGGATCTCTCCAGAATCCCTATGGAATGACCTTCC

AACCGCCACCGCCGACACCCAGCGCCCGCCAAGTGCCCGAGCCAGGGGAGCGCAGCCGGACCATGCCTCG

CACCGTGCCCGGCTCTACCATGAAGATGGGCTCCCTGGAGCGAAAGAAATTACGGTATTCAGACCTGGAC

TTTGAGGTGATGCACACCCGGAAACGGCATTCAGAACTCTACCACGAGCTCAACCAGAAGTTCCACACTT

TCGACCGCTACCGCAGCCAGTCCACGGCCAAGAGGGAGAAGCGGTGGAGTGTGTCCTCGGGTGGGGCAGC

CGAGCGGAGCGTGTGCACCGATAAGCCCAGCCCTGGGGAGCGCCCCAGCTTGTCCCAACATCGGCGCCAT

CAGAGCTGGAGCACCTTCAAATCTATGACACTGGGCTCGCTGCCCCCCAAGCCCCGAGAACGGCTGACTC

TGCACCGGGCAGCAGCCTGGGAGCCCACAGAACCACCGGATGGTGACTTCCAGACAGAGGTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.