CD4, also known as T-cell surface antigen T4, Leu-3, L3T4, and W3/25, is a single-pass type I transmembrane glycoprotein of the immunoglobulin superfamily (IgSF). CD4 contains 3 Ig- like C2-type (IgC2) domains and 1 Ig-like V-type (IgV) domain in its extracellular region. CD4 is expressed predominantly on thymocytes and a subset of mature T lymphocytes.  CD4 serves as a standard marker for the identification of T cell populations.  CD4 is expressed along with CD8 on T cells during their early development in the thymus. CD4 or CD8 expression is then lost, giving rise to single positive CD4+ or CD8+ mature T cells. CD4+ cells, also known as T helper (Th) cells, further differentiate into multiple subsets including Th1, Th2, Th17, Tfh, and Treg cells, which are crucial in the regulation of humoral and cellular immunity. In human, CD4 is also expressed on macrophages, neutrophils, monocytes, NK cells, dendritic cells and glial cells in the brain.  CD4 is a co-receptor that assists the T cell receptor (TCR) to activate T cells following the interaction with MHC class II molecules on antigen presenting cells (APC). CD4 also functions as a chemotactic receptor for IL­16. In human, CD4 also acts as a co-receptor for the gp120 surface glycoprotein of HIV­1. CD4 is a primary receptor by HIV-1 to gain entry into host T cells. 

CD4, also known as T-cell surface antigen T4, Leu-3, L3T4, and W3/25, is a single-pass type I transmembrane glycoprotein of the immunoglobulin superfamily (IgSF). CD4 contains 3 Ig- like C2-type (IgC2) domains and 1 Ig-like V-type (IgV) domain in its extracellular region. CD4 is expressed predominantly on thymocytes and a subset of mature T lymphocytes.  CD4 serves as a standard marker for the identification of T cell populations.  CD4 is expressed along with CD8 on T cells during their early development in the thymus. CD4 or CD8 expression is then lost, giving rise to single positive CD4+ or CD8+ mature T cells. CD4+ cells, also known as T helper (Th) cells, further differentiate into multiple subsets including Th1, Th2, Th17, Tfh, and Treg cells, which are crucial in the regulation of humoral and cellular immunity. In human, CD4 is also expressed on macrophages, neutrophils, monocytes, NK cells, dendritic cells and glial cells in the brain.  CD4 is a co-receptor that assists the T cell receptor (TCR) to activate T cells following the interaction with MHC class II molecules on antigen presenting cells (APC). CD4 also functions as a chemotactic receptor for IL­16. In human, CD4 also acts as a co-receptor for the gp120 surface glycoprotein of HIV­1. CD4 is a primary receptor by HIV-1 to gain entry into host T cells. 

 

Product Details

 

Gene Symbol: CD4; T4/Leu-3; CD4mut;

 

NCBI Gene ID: 920

 

Uniprot Entry: P01730

 

Construct Details: The recombinant human CD4-Fc fusion is expressed as a 599 amino acid protein consisting of Lys26 - Pro396 region of CD4 (Uniprot Accession #P01730) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD4-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS 

        70         80         90        100        110        120 

LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES 

       130        140        150        160        170        180 

PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ 

       190        200        210        220        230        240 

KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LKNKEVSVKR 

       250        260        270        280        290        300 

VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLTLALEAKT GKLHQEVNLV VMRATQLQKN 

       310        320        330        340        350        360 

LTCEVWGPTS PKLMLSLKLE NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK 

       370        380        390        400        410        420 

VLPTWSTPVQ PSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH 

       430        440        450        460        470        480 

EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL 

       490        500        510        520        530        540 

PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE 

       550        560        570        580        590 

NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 66.9; Estimated by SDS-PAGE under reducing condition (kDa): 70-75

 

Calculated PI: 8.88

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 95630

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to GP140 and supports the adhesion of mammalian cells such as mouse embryonic fibroblasts and human cervical epithelial carcinoma HeLa cells.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nature 312:763 & 767 (1984)

2. Cell 42:93 (1985)

3. Proc. Natl. Acad. Sci. 83: 4399 (1986)

4. Nature 330:256 (1987)

5. Structure 2 : 59 (1994)

6. Nature 463:963 (2010)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2275B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.