IL2Rα (interleukin 2 receptor α), also known as CD25 and TAC antigen, is a single-pass type I transmembrane glycoprotein of the IL2R family. IL2Rα lacks structural features characteristic of members of the cytokine receptor superfamily. It contains 2 Sushi (CCP/SCR) domains in the extracellular region. IL2Rα is expressed on activated T cells and regulatory T cells. It is capable of binding IL2 with low affinity by itself.The heterodimer of IL2Rα and IL2Rβ together with the common γ chain IL2Rγ constitute the high-affinity IL2 receptor that transduces IL2 signals, leading to the activation of the intracellular signal pathways including MAPK, PI3K and JAK/STAT. The IL2R complex therefore regulates T cell proliferation, activation and other lymphoid functions.  A soluble form of IL2Rα (IL2sRα) appears in human serum. However, the function of IL2sRα is unclear. Increased levels of IL2sRα in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum levels of IL­2 sRα have been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas. Mutations in this gene are associated with immunodeficiency due to IL2Rα/CD25 deficiency.  The IL2Rα gene variations are also associated with diseases susceptibility of insulin-dependent diabetes mellitus.

IL2Rα (interleukin 2 receptor α), also known as CD25 and TAC antigen, is a single-pass type I transmembrane glycoprotein of the IL2R family. IL2Rα lacks structural features characteristic of members of the cytokine receptor superfamily. It contains 2 Sushi (CCP/SCR) domains in the extracellular region. IL2Rα is expressed on activated T cells and regulatory T cells. It is capable of binding IL2 with low affinity by itself.The heterodimer of IL2Rα and IL2Rβ together with the common γ chain IL2Rγ constitute the high-affinity IL2 receptor that transduces IL2 signals, leading to the activation of the intracellular signal pathways including MAPK, PI3K and JAK/STAT. The IL2R complex therefore regulates T cell proliferation, activation and other lymphoid functions.  A soluble form of IL2Rα (IL2sRα) appears in human serum. However, the function of IL2sRα is unclear. Increased levels of IL2sRα in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum levels of IL­2 sRα have been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas. Mutations in this gene are associated with immunodeficiency due to IL2Rα/CD25 deficiency.  The IL2Rα gene variations are also associated with diseases susceptibility of insulin-dependent diabetes mellitus.

 

Product Details

 

Gene Symbol: CD25; IL2Rα; IL2RA; Tac; p55; IL2R; TCGFR; IDDM10

 

NCBI Gene ID: 3559

 

Uniprot Entry: P01589

 

Construct Details: The recombinant human CD25-Fc fusion is expressed as a 457 amino acid protein consisting of Glu22 -  Gln240 region of CD25 (Uniprot Accession #P01589) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD25-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ 

        70         80         90        100        110        120 

CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH 

       130        140        150        160        170        180 

FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA 

       190        200        210        220        230        240 

SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQS TTENLYFQGS TGTHTCPPCP 

       250        260        270        280        290        300 

APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK 

       310        320        330        340        350        360 

PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 

       370        380        390        400        410        420 

LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL 

       430        440        450 

TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 51.5; Estimated by SDS-PAGE under reducing condition (kDa): 60-75

 

Calculated PI: 5.87 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 64830

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds human IL2 and blocks IL2-mediated signaling activities, including IL­2­dependent proliferation of human megakaryocytic leukemic cells with a typical ED₅₀ of 0.1 - ­0.5 μg/mL

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nature 311:626, 631 (1983)

2. Science 230:633 (1984)

3. Mol. Cell. Biol. 19: 2681 (1999)

4. Nat. Genet. 39:1074 (2006)

5. J. Immunol. 181: 1673 (2008)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2130-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.