CD146, also known as MUC18 and MCAM, is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. CD146 contains 2 Ig-like V-type domains and 3 Ig-like C2-type domains (V-V-C2-C2-C2) in the extracellular region (ECD) in addition to a transmembrane (TM) region and a short cytoplasmic tail. CD146 is expressed in endothelial cells in vascular tissue throughout the body. Its expression appears to be limited to vascular smooth muscle in normal adult tissues. CD146 is involved in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 is also an adhesion molecule active in neural crest cells during embryonic development. CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 can act as a cell surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1 as well as a transient increase in the intracellular calcium concentration. CD146 is expressed most strongly on metastatic lesions and advanced primary tumors while it is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.  It thus suggests that CD146 may play an important role in tumor progression and metastasis.

CD146, also known as MUC18 and MCAM, is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. CD146 contains 2 Ig-like V-type domains and 3 Ig-like C2-type domains (V-V-C2-C2-C2) in the extracellular region (ECD) in addition to a transmembrane (TM) region and a short cytoplasmic tail. CD146 is expressed in endothelial cells in vascular tissue throughout the body. Its expression appears to be limited to vascular smooth muscle in normal adult tissues. CD146 is involved in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 is also an adhesion molecule active in neural crest cells during embryonic development. CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 can act as a cell surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1 as well as a transient increase in the intracellular calcium concentration. CD146 is expressed most strongly on metastatic lesions and advanced primary tumors while it is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.  It thus suggests that CD146 may play an important role in tumor progression and metastasis.

 

Product Details

 

Gene Symbol: CD146; MUC18; MCAM; P1H12; A32

 

NCBI Gene ID: 4162

 

Uniprot Entry: P43121

 

Construct Details: Full length human CD146/MUC18/MCAM gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1941 (bp)

 

Gene Insert Sequence:  

ATGGGGCTTCCCAGGCTGGTCTGCGCCTTCTTGCTCGCCGCCTGCTGCTGCTGTCCTCGCGTCGCGGGTGTGCCCGGAGA

GGCTGAGCAGCCTGCGCCTGAGCTGGTGGAGGTGGAAGTGGGCAGCACAGCCCTTCTGAAGTGCGGCCTCTCCCAGTCCC

AAGGCAACCTCAGCCATGTCGACTGGTTTTCTGTCCACAAGGAGAAGCGGACGCTCATCTTCCGTGTGCGCCAGGGCCAG

GGCCAGAGCGAACCTGGGGAGTACGAGCAGCGGCTCAGCCTCCAGGACAGAGGGGCTACTCTGGCCCTGACTCAAGTCAC

CCCCCAAGACGAGCGCATCTTCTTGTGCCAGGGCAAGCGCCCTCGGTCCCAGGAGTACCGCATCCAGCTCCGCGTCTACA

AAGCTCCGGAGGAGCCAAACATCCAGGTCAACCCCCTGGGCATCCCTGTGAACAGTAAGGAGCCTGAGGAGGTCGCTACC

TGTGTAGGGAGGAACGGGTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCTCTGAAGGAGGAGAAGAACCG

GGTCCACATTCAGTCGTCCCAGACTGTGGAGTCGAGTGGTTTGTACACCTTGCAGAGTATTCTGAAGGCACAGCTGGTTA

AAGAAGACAAAGATGCCCAGTTTTACTGTGAGCTCAACTACCGGCTGCCCAGTGGGAACCACATGAAGGAGTCCAGGGAA

GTCACCGTCCCTGTTTTCTACCCGACAGAAAAAGTGTGGCTGGAAGTGGAGCCCGTGGGAATGCTGAAGGAAGGGGACCG

CGTGGAAATCAGGTGTTTGGCTGATGGCAACCCTCCACCACACTTCAGCATCAGCAAGCAGAACCCCAGCACCAGGGAGG

CAGAGGAAGAGACAACCAACGACAACGGGGTCCTGGTGCTGGAGCCTGCCCGGAAGGAACACAGTGGGCGCTATGAATGT

CAGGGCCTGGACTTGGACACCATGATATCGCTGCTGAGTGAACCACAGGAACTACTGGTGAACTATGTGTCTGACGTCCG

AGTGAGTCCCGCAGCCCCTGAGAGACAGGAAGGCAGCAGCCTCACCCTGACCTGTGAGGCAGAGAGTAGCCAGGACCTCG

AGTTCCAGTGGCTGAGAGAAGAGACAGGCCAGGTGCTGGAAAGGGGGCCTGTGCTTCAGTTGCATGACCTGAAACGGGAG

GCAGGAGGCGGCTATCGCTGCGTGGCGTCTGTGCCCAGCATACCCGGCCTGAACCGCACACAGCTGGTCAACGTGGCCAT

TTTTGGCCCCCCTTGGATGGCATTCAAGGAGAGGAAGGTGTGGGTGAAAGAGAATATGGTGTTGAATCTGTCTTGTGAAG

CGTCAGGGCACCCCCGGCCCACCATCTCCTGGAACGTCAACGGCACGGCAAGTGAACAAGACCAAGATCCACAGCGAGTC

CTGAGCACCCTGAATGTCCTCGTGACCCCGGAGCTGTTGGAGACAGGTGTTGAATGCACGGCCTCCAACGACCTGGGCAA

AAACACCAGCATCCTCTTCCTGGAGCTGGTCAATTTAACCACCCTCACACCAGACTCCAACACAACCACTGGCCTCAGCA

CTTCCACTGCCAGTCCTCATACCAGAGCCAACAGCACCTCCACAGAGAGAAAGCTGCCGGAGCCGGAGAGCCGGGGCGTG

GTCATCGTGGCTGTGATTGTGTGCATCCTGGTCCTGGCGGTGCTGGGCGCTGTCCTCTATTTCCTCTATAAGAAGGGCAA

GCTGCCGTGCAGGCGCTCAGGGAAGCAGGAGATCACGCTACCCCCGTCTCGTAAGAGCGAACTTGTAGTTGAAGTTAAGT

CAGATAAGCTCCCAGAAGAGATGGGCCTCCTGCAGGGCAGCAGCGGTGACAAGAGGGCTCCGGGAGACCAGGGAGAGAAA

TACATCGATCTGAGGCATTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 86:9891 (1988)

2. Melanoma Res. 3:337 (1992)

3. J. Biol. Chem. 276:1564 (2000) 

4. Cancer Res. 54:2514 (1993)

5. J. Immunol. 179: 6673 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2150-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.