Killer cell immunoglobulin-like receptors (KIRs), also known as Killer-cell Inhibitory Receptors, are transmembrane glycoproteins that belong to the immunoglobulin superfamily (IgSF).  The KIR genes are highly homologous and polymorphic.  Most of them are found in a cluster on chromosome 19q13.4 within the leukocyte receptor complex (LRC). KIRs are expressed on natural killer (NK) cells and subsets of T cells. KIR proteins are composed of two or three extracellular immunoglobulin (Ig) domains, a transmembrane region and cytoplasmic tail which can in turn be short or long. The KIR proteins are thus classified by the number of Ig-domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM).  In contrast KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. KIR2DL subgroup proteins, including KIR2DL1 (CD158A), KIR2DL2 (CD158B1), and KIR2DL3 (CD158B2), are receptors on NK cells for HLA-C alleles and inhibit the activity of NK cells thus preventing cell lysis.

Killer cell immunoglobulin-like receptors (KIRs), also known as Killer-cell Inhibitory Receptors, are transmembrane glycoproteins that belong to the immunoglobulin superfamily (IgSF).  The KIR genes are highly homologous and polymorphic.  Most of them are found in a cluster on chromosome 19q13.4 within the leukocyte receptor complex (LRC). KIRs are expressed on natural killer (NK) cells and subsets of T cells. KIR proteins are composed of two or three extracellular immunoglobulin (Ig) domains, a transmembrane region and cytoplasmic tail which can in turn be short or long. The KIR proteins are thus classified by the number of Ig-domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM).  In contrast KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. KIR2DL subgroup proteins, including KIR2DL1 (CD158A), KIR2DL2 (CD158B1), and KIR2DL3 (CD158B2), are receptors on NK cells for HLA-C alleles and inhibit the activity of NK cells thus preventing cell lysis.

 

Product Details

 

Gene Symbol: CD158B2; p58; NKAT; GL183; NKAT2; CD158b; NKAT2A; NKAT2B; KIR-K7b; KIR-K7c; KIR2DS5; KIRCL23; KIR-023GB

 

NCBI Gene ID: 3804

 

Uniprot Entry: P43628

 

Construct Details: The recombinant human KIR2DL3-Fc fusion is expressed as a 449 amino acid protein consisting of His22 - Arg242 region of KIR2DL3 (UniProt accession #P43628)  and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted).  

  

Source:  Human cells stably expressing KIR2DL3-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

 

        10         20         30         40         50         60 

HEGVHRKPSL LAHPGPLVKS EETVILQCWS DVRFQHFLLH REGKFKDTLH LIGEHHDGVS 

        70         80         90        100        110        120 

KANFSIGPMM QDLAGTYRCY GSVTHSPYQL SAPSDPLDIV ITGLYEKPSL SAQPGPTVLA 

       130        140        150        160        170        180 

GESVTLSCSS RSSYDMYHLS REGEAHERRF SAGPKVNGTF QADFPLGPAT HGGTYRCFGS 

       190        200        210        220        230        240 

FRDSPYEWSN SSDPLLVSVT GNPSNSWLSP TEPSSETGNP RSTGTHTCPP CPAPELLGGP 

       250        260        270        280        290        300 

SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS 

       310        320        330        340        350        360 

TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM 

       370        380        390        400        410        420 

TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ 

       430        440 

QGNVFSCSVM HEALHNHYTQ KSLSLSPGK 

 

M.W.: Calculated molecular mass 49.8 kDa; estimated by SDS-PAGE under reducing condition 60-65 kDa probably due to glycosylation. 

 

Calculated PI:  6.46 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm):  64455

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds anti-KIR monoclonal antibodies, human IgG4 (SKU#MAB1939), human IgG1 (SKU#MAB2024) and rabbit IgG (SKU#MAB2027).

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Science 268:405 (1995)

2. Immunity 2:439 (1995) 

3. Nature. 389:96 (1997)

4. Immunity 7:753 (1997)

5. Annu. Rev. Immunol. 23:225 (2005)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2044-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.