Human PD-L1 protein (extracellular domain), recombinant

Product ID: PD-L1 ECD (SKU#: FCL0784)

Price:

$397.00

Description

PD-L1 (programmed cell death 1 ligand 1) or CD274 is a 290-amino acid single pass type I transmembrane protein of the Ig (immunoglobulin) superfamily. It contains one Ig V-like and one Ig C-like domains in the extracellular region, and a 30-amino acid cytoplasmic tail. It was originally cloned as a homolog of B7 (termed B7-H1). It is 20% and 15% identical to B7-1 (CD80) and B7-2 (CD86), respectively. It was also cloned as a ligand (termed PDCD1L1 or PD-L1) that binds to PD-1 (CD279) and B7-1 (CD80), but not to other B7 family members, such as CTLA4 (CD152), CD28, or ICOS (CD278). PD-L1 or CD274 is involved in the T cell costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNγ. Interaction with PD-1, which has a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), inhibits T-cell proliferation and cytokine production. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNγ activation or by surface Ig cross-linking. It is expressed in many types of freshly isolated human cancers but not in most normal tissues. Blockade of PD-L1 upregulates IL12 expression and enhances antitumor immunity in mice bearing ovarian tumors. Therefore, PD-L1 has been proposed as a target for cancer immunotherapy through pre-activated T cells that could be enhanced by blockade of PD-L1-induced inhibitory signaling.

Product Details

Gene Symbol: PD-L1; CD274; B7-H1; B7H1; PDL1; PDCD1L1; PDCD1LG1

NCBI Gene ID: 29126

Uniprot Entry: Q9NZQ7

Construct Details: The recombinant human PD-L1 or CD274 ECD is expressed as a 231 amino acid protein consisting of Phe19 - Arg238 region of CD274 (UniProt accession #Q9NZQ7) and a C-terminal His-tag. It contains 4 potential sites for N-linked glycosylation

Source: Human cells stably expressing PD-L1 ECD and growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVK

LQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFN

VTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERSTGHHHHHHHH

M.W.: Calculated molecular mass 26.5kDa; estimated by SDS-PAGE under reducing condition ~35 kDa with higher molecular mass smear resembling different glycosylation states

Calculated PI: 6.27

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 26,150

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Binds to its receptor PD-1 extracellular domain proteins (SKU# FCL0761 and FCL0763) and PD-1 molecule on cell surface with low affinity (K_D in μM range) and anti-PD-L1 monoclonal antibody (SKU# MAB0199) with high affinity(K_D < 1 nM) as measured by ELISA (see Technical Data).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Technical Data

Recombinant PD-L1 ECD protein (SKU#FCL0784) was immobilized at 150 ng/well on a 96-well NuncMaxisorp plate for an ELISA with serially diluted anti-PD-L1 monoclonal antibody (SKU#MAB0199). Anti-PD-1 (SKU#MA1732) monoclonal antibody was used as a negative control to demonstrate the specificity of PD-L1 binding under the experimental conditions. The binding curve was fit and apparent K_D was calculated for anti-PD-L1 : PD-L1 binding to be 21.6 ± 1.1 pM.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References