EPCAM (Epithelial Cellular Adhesion Molecule), also known as CD326, EGP, KS1/4, KSA, gp40, GA733­2, 17­1A, MIC18 and TROP-1, is a single-pass type I transmembrane glycoprotein.  It is composed of a 242 amino acid (aa) extracellular domain (ECD) with 2 EGF-like repeats, a transmembrane domain, and a 26 aa cytoplasmic domain. EPCAM is expressed on most normal epithelial cells and functions as a homotypic calcium-independent cell adhesion molecule. Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers. EPCAM may act as a physical homophilic interaction molecule at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It also plays a role in embryonic stem cells proliferation and differentiation. EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration. EpCAM expression is increased in human malignant neoplasias, with most carcinoma expressing EpCAM. Mutations in this gene result in congenital tufting enteropathy. EPCAM is a target for immunotherapy treatment of human carcinomas. The anti-EPCAM bispecific antibody recognizes both EPCAM and CD3e thus promoting T-cell-mediated anti-tumor activity after engaging with tumor surface EPCAM antigen.

EPCAM (Epithelial Cellular Adhesion Molecule), also known as CD326, EGP, KS1/4, KSA, gp40, GA733­2, 17­1A, MIC18 and TROP-1, is a single-pass type I transmembrane glycoprotein.  It is composed of a 242 amino acid (aa) extracellular domain (ECD) with 2 EGF-like repeats, a transmembrane domain, and a 26 aa cytoplasmic domain. EPCAM is expressed on most normal epithelial cells and functions as a homotypic calcium-independent cell adhesion molecule. Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers. EPCAM may act as a physical homophilic interaction molecule at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It also plays a role in embryonic stem cells proliferation and differentiation. EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration. EpCAM expression is increased in human malignant neoplasias, with most carcinoma expressing EpCAM. Mutations in this gene result in congenital tufting enteropathy. EPCAM is a target for immunotherapy treatment of human carcinomas. The anti-EPCAM bispecific antibody recognizes both EPCAM and CD3e thus promoting T-cell-mediated anti-tumor activity after engaging with tumor surface EPCAM antigen.

 

Product Details

 

Gene Symbol: CD326; ESA; KSA; EGP-2; EGP40; TROP1; EGP314; HNPCC8; TACSTD1; CD3E; T3E; TCRE; IMD18; Leu-4

 

NCBI Gene ID: 4072; 916 

 

Uniprot Entry: P16422; P07766

 

Construct Details: Expressed as a single-chain Fv (scFv) from anti-EPCAM VH and VL region fused with a scFv of anti-CD3e VH and VL region with a His-tag at the C-terminus. Exists as a monomer under non-reducing and reducing condition.

 

Source: Mammalian cells stably expressing anti-EPCAM:anti-CD3e bispecific antibody (in a scFv-scFv’ format) and growing in chemical-defined media with no animal component or antibiotics.

 

M.W.: Calculated molecular mass (kDa): 55.8. Estimated by SDS-PAGE under non-reducing condition(kDa): 55-60. Calculated extinction coefficients (M-1 cm-1, at 280nm):  121170.

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Recognizes EPCAM on tumor cells and CD3e in the context of TCR complex on T cells.  Potently promotes T cell-mediated anti-tumor activity in vitro and in vivo after engaging with tumor cell surface antigen EPCAM. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

  

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 87: 2755 (1990) 

2. J. Cell. Biol. 125: 437 (1994)

3. Mol. Cell. Biol. 18:4388 (1998)

4. J. Biol. Chem. 276: 5804 (2001)

5. Cancer. Res. 67: 10831 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2028-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.