Connective Tissue Growth Factor (CTGF) is a member of the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a multimodular organization. Each CCN member contains an IGF­binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF binds multiple ligands and shows effects in development, differentiation, and tumorigenesis. CTGF is a mitogen that is secreted by vascular endothelial cells. It plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. The C­terminal cysteine knot motif contains the heparin and low-density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.  Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. CTGF is the central mediator of tissue remodeling and fibrosis.  It is implicated in tumor progression and metastasis.  It promotes epithelial to mesenchymal transition (EMT), a process whereby normal epithelial cells become migratory, matrix-producing fibroblasts important for invasion and metastasis.  Elevated CTGF levels are found in desmoplastic cancer where it supports the formation of the extensive fibrous connective tissue (desmoplasia) around cancer.  Blockade of the expression or activity of CTGF reduces tumor growth and metastasis in serveral cancer animal model.

Connective Tissue Growth Factor (CTGF) is a member of the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a multimodular organization. Each CCN member contains an IGF­binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF binds multiple ligands and shows effects in development, differentiation, and tumorigenesis. CTGF is a mitogen that is secreted by vascular endothelial cells. It plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. The C­terminal cysteine knot motif contains the heparin and low-density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.  Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. CTGF is the central mediator of tissue remodeling and fibrosis.  It is implicated in tumor progression and metastasis.  It promotes epithelial to mesenchymal transition (EMT), a process whereby normal epithelial cells become migratory, matrix-producing fibroblasts important for invasion and metastasis.  Elevated CTGF levels are found in desmoplastic cancer where it supports the formation of the extensive fibrous connective tissue (desmoplasia) around cancer.  Blockade of the expression or activity of CTGF reduces tumor growth and metastasis in serveral cancer animal model.

 

Product Details

 

Gene Symbol: CCN2; NOV2; HCS24; IGFBP; IBP-8; IGFBP-8

 

NCBI Gene ID: 1490

 

Uniprot Entry: Q5M8T4

 

Construct Details: Expressed as the combination of a heavy chain (HC) containing VH from anti-CTGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-CTGF mAb and CL of human kappa light chain.  Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition (see the gel image above, labeled as DTT "-").

 

Source: Mammalian cells stably expressing anti-CTGF human IgG1 HC and LC and growing in chemical-defined media with no animal component or antibiotics.

 

M.W.: Calculated molecular mass (kDa): 146 (49 kDa for a single HC, 24 kDa for a single LC); Estimated by SDS-PAGE under non-reducing condition(kDa): 150

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 1.0 mg/ml in sterile PBSpH7.4 (carrier free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human CTGF protein with high affinity and blocks CTGF-mediated signaling activity. Has the same binding specificity as anti-CTGF, rabbit IgG ( SKU#MAB1998).

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Cell Biol. 114:1285 (1991).

2. J Cell Physiol. 181:153 (1999).

3. Endocr. Rev. 20:189 (1999).

4. Cancer Res. 66: 5817 (2006)

5. J. Hum. Genet. 51: 383 (2006) 

6. Dev. Cell 14: 818 (2008) 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: MAB1466-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.