EDA2R (ectodysplasin A2 receptor), also known as XEDAR (X­linked Ectodysplasin Receptor), is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF27). The extracellular domain (ECD) of EDA2R contains three TNFR cysteine­rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. The cytoplasmic region of EDA2R lacks a death domain (DD) that is found in EDAR and other TNFRSF members. EDA2R is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Other than a 2 aa deletion in its TNF­like domain, EDA­A2 is identical to EDA­A1. Mutations in EDA give rise to a clinical syndrome hypohidrotic ectodermal dysplasia (HED), which is characterized by loss of hair, sweat glands, and teeth. EDA2R specifically binds to EDA-A2 isoform but not EDA-A1. EDA2R itself is strongly associated with androgenetic alopecia (male hair loss). Even though it does not contain a cytoplasmic death domain, EDA2R can associate with Fas and induce p53-dependent cell death. EDA2R is down-regulated in breast, colon, and lung cancers, particularly in patients with p53 mutations. EDA2R also plays a role in EDA-A2 induced skeletal muscle degeneration and osteoblast differentiation.

EDA2R (ectodysplasin A2 receptor), also known as XEDAR (X­linked Ectodysplasin Receptor), is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF27). The extracellular domain (ECD) of EDA2R contains three TNFR cysteine­rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. The cytoplasmic region of EDA2R lacks a death domain (DD) that is found in EDAR and other TNFRSF members. EDA2R is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Other than a 2 aa deletion in its TNF­like domain, EDA­A2 is identical to EDA­A1. Mutations in EDA give rise to a clinical syndrome hypohidrotic ectodermal dysplasia (HED), which is characterized by loss of hair, sweat glands, and teeth. EDA2R specifically binds to EDA-A2 isoform but not EDA-A1. EDA2R itself is strongly associated with androgenetic alopecia (male hair loss). Even though it does not contain a cytoplasmic death domain, EDA2R can associate with Fas and induce p53-dependent cell death. EDA2R is down-regulated in breast, colon, and lung cancers, particularly in patients with p53 mutations. EDA2R also plays a role in EDA-A2 induced skeletal muscle degeneration and osteoblast differentiation.

  

Product Details

 

Gene Symbol: XEDAR; EDAA2R; EDA-A2R; TNFRSF27; UNQ2448/PRO5727/PRO34080

  

NCBI Gene ID: 60401

 

Uniprot Entry: Q9HAV5

 

Construct Details: The recombinant human EDA2R-Fc fusion is expressed as a 366 amino acid protein consisting of Asp2 - Thr138 region of EDA2R (UniProt accession #Q9HAV5) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

   

Source: Human cells stably expressing human EDA2R-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

MDCQENEYWD QWGRCVTCQR CGPGQELSKD CGYGEGGDAY CTACPPRRYK SSWGHHRCQS 

        70         80         90        100        110        120 

CITCAVINRV QKVNCTATSN AVCGDCLPRF YRKTRIGGLQ DQECIPCTKQ TPTSEVQCAF 

       130        140        150        160        170        180 

QLSLVEADTP TVPPQEATST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC 

       190        200        210        220        230        240 

VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC 

       250        260        270        280        290        300 

KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW 

       310        320        330        340        350        360 

ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL 

 

SLSPGK 

 

M.W.: Calculated molecular mass (kDa): 40.9; Estimated by SDS-PAGE under reducing condition (kDa): 50-55

 

Calculated PI: 6.74

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 60735

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand EDA­-A2 and blocks EDA-A2-mediated signaling activity.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Science 290:523 (2000).

2. J. Biol. Chem. 277:44953 (2002).

3. Mol. Cell. Biol. 24:1608 (2004).

4. Oncogene 28:3081 (2009).

5. Clin. Cancer Res. 16:1140 (2010). 

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0301B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.