DR3 (death receptor 3), also known as AIR, LARD, WSL­1, APO3, TRAMP and TR3, is a single pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF25). DR3 contains an extracellular region with 4 TNFR cysteine­rich repeats and a cytoplasmic region with 1 death domain (DD) that is also found in other 5 TNFRSF members. DR3 is most closely related to TNFR1 and FAS/CD95, sharing 29% and 23% amino acid sequence identity, respectively.  DR3 is abundantly expressed in thymocytes and lymphocytes and detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. It is also found in the prostate. Naïve B and T cells express multiple truncated DR3 isoforms that lack the transmembrane domain. Upon T cell activation, expression of the transmembrane isoform 1 of DR3 predominates. DR3 interacts directly with the adapter protein TRADD to mediate activation of NF-κB and induce apoptosis. DR3 may play a role in regulating lymphocyte homeostasis. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. TL1A/TNFSF15, a TNF superfamily ligand, has been shown to bind and activate DR3. On T cells, TL1A induces a costimulatory signal to increase IL­2 responsiveness and the secretion of proinflammatory cytokines. TL1A has also been shown to induce caspase activity and apoptosis.

DR3 (death receptor 3), also known as AIR, LARD, WSL­1, APO3, TRAMP and TR3, is a single pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF25). DR3 contains an extracellular region with 4 TNFR cysteine­rich repeats and a cytoplasmic region with 1 death domain (DD) that is also found in other 5 TNFRSF members. DR3 is most closely related to TNFR1 and FAS/CD95, sharing 29% and 23% amino acid sequence identity, respectively.  DR3 is abundantly expressed in thymocytes and lymphocytes and detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. It is also found in the prostate. Naïve B and T cells express multiple truncated DR3 isoforms that lack the transmembrane domain. Upon T cell activation, expression of the transmembrane isoform 1 of DR3 predominates. DR3 interacts directly with the adapter protein TRADD to mediate activation of NF-κB and induce apoptosis. DR3 may play a role in regulating lymphocyte homeostasis. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. TL1A/TNFSF15, a TNF superfamily ligand, has been shown to bind and activate DR3. On T cells, TL1A induces a costimulatory signal to increase IL­2 responsiveness and the secretion of proinflammatory cytokines. TL1A has also been shown to induce caspase activity and apoptosis.

  

Product Details

 

Gene Symbol: TNFRSF25; AIR; TR3; DDR3; LARD; APO-3; TRAMP; WSL; WSL1; WSL-1; WSL-LR; TNFRSF12; UNQ455/PRO779

  

NCBI Gene ID: 8718

 

Uniprot Entry: Q93038

 

Construct Details: The recombinant human DR3-Fc fusion is expressed as a 403 amino acid protein consisting of Gln25 - Gln199 region of DR3 (UniProt accession #Q93038) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

   

Source: Human cells stably expressing human DR3-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QGGTRSPRCD CAGDFHKKIG LFCCRGCPAG HYLKAPCTEP CGNSTCLVCP QDTFLAWENH  

        70         80         90        100        110        120 

HNSECARCQA CDEQASQVAL ENCSAVADTR CGCKPGWFVE CQVSQCVSSS PFYCQPCLDC 

       130        140        150        160        170        180 

GALHRHTRLL CSRRDTDCGT CLPGFYEHGD GCVSCPTSTL GSCPERCAAV CGWRQSTGTH 

       190        200        210        220        230        240 

TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV 

       250        260        270        280        290        300 

HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR 

       310        320        330        340        350        360 

EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF 

       370        380        390        400 

FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK

 

M.W.: Calculated molecular mass (kDa): 44.5; Estimated by SDS-PAGE under reducing condition (kDa): 55-60

 

Calculated PI: 6.72

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 58505

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand human TL1A/TNFSF15 and blocks TL1A-mediated signaling activity including TL1A-induced apoptosis in human tumor cell line TF-1.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Science 274:990 (1996).

2. Nature 384:372 (1996).

3. Curr. Biol. 6:1669 (1996).

4. Immunity 6:79 (1997).

5. Immunity 16:479 (2002).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCLSF56B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.