The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. BMPR2 (BMP receptor type 2), also known as PPH1 and BMPR-II, is a type II receptor for BMPs that are involved in endochondral bone formation and embryogenesis. The type II TGFRs typically bind ligands with high affinity. In contrast, BMPR2 binds BMPs weakly in the absence of type I receptor, and the binding is enhanced by the presence of the type I receptor. BMPR2 is widely expressed in fetal and adult tissues.  Reduced expression or function of BMPR2 signaling leads to exaggerated TGFβ signaling and altered cellular response. BMPR2 may contribute to the maintenance of normal pulmonary vascular structure and function. Mutations in the BMPR2 gene are associated with primary pulmonary hypertension (PPH1) and pulmonary venoocclusive disease 1 (PVOD1).  Human and mouse BMPR2 are 97% identical and show cross-species binding.

The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. BMPR2 (BMP receptor type 2), also known as PPH1 and BMPR-II, is a type II receptor for BMPs that are involved in endochondral bone formation and embryogenesis. The type II TGFRs typically bind ligands with high affinity. In contrast, BMPR2 binds BMPs weakly in the absence of type I receptor, and the binding is enhanced by the presence of the type I receptor. BMPR2 is widely expressed in fetal and adult tissues.  Reduced expression or function of BMPR2 signaling leads to exaggerated TGFβ signaling and altered cellular response. BMPR2 may contribute to the maintenance of normal pulmonary vascular structure and function. Mutations in the BMPR2 gene are associated with primary pulmonary hypertension (PPH1) and pulmonary venoocclusive disease 1 (PVOD1).  Human and mouse BMPR2 are 97% identical and show cross-species binding.

 

Product Details

 

Gene Symbol: BMPR2; BMR2; PPH1; BMPR-2; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II; BMPRII

 

NCBI Gene ID: 659

 

Uniprot Entry: Q13873

 

Construct Details: The recombinant human BMPR2-Fc fusion protein is expressed as a 352 amino acid protein consisting of Ser27 - Thr150 region of BMPR2 (UniProt accession #Q13873) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as "DTT: -")

 

Source: Human cells stably expressing human BMPR2-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

SQNQERLCAFKDPYQQDLGIGESRISHENGTILCSKGSTCYGLWEKSK

GDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCSTDL

CNVNFTENFPPPDTTPLSPPHSFNRDETSTGTHTCPPCPAPELLGGPS

VFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNA

KTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKT

ISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWES

NGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEA

LHNHYTQKSLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 39.5; Estimated by SDS-PAGE under reducing condition (kDa): ~55

 

Calculated PI: 5.90

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  53370

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Recombinant BMPR2 protein binds human/mouse BMP2/BMP7/GDF9, and blocks BMP2/BMP7/GDF9-induced signaling activity

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 270:5625 (1995)

2. Proc. Natl. Acad. Sci. 92:7632 (1995). 

3. Am. J. Hum. Genet. 67:737 (2000).

4. Nat. Genet. 26:81 (2000),

5. Hum. Mol. Genet. 12: 3277 (2003). 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0116B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.