ROR2 (receptor tyrosine kinase-like orphan receptor 2), also known as NTRKR2 (neurotrophic tyrosine kinase, receptor-related 2), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR2 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.  ROR2 may be involved in the early formation of the chondrocytes and required for cartilage and growth plate development. ROR2 exists as a homodimer on the cell surface and may mediate a signaling pathway that promotes osteogenesis and bone formation. Mutations in the ROR2 gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. ROR2 appears to possess dual roles as a tumor suppressor or activator depending on tumor type.

ROR2 (receptor tyrosine kinase-like orphan receptor 2), also known as NTRKR2 (neurotrophic tyrosine kinase, receptor-related 2), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR2 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.  ROR2 may be involved in the early formation of the chondrocytes and required for cartilage and growth plate development. ROR2 exists as a homodimer on the cell surface and may mediate a signaling pathway that promotes osteogenesis and bone formation. Mutations in the ROR2 gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. ROR2 appears to possess dual roles as a tumor suppressor or activator depending on tumor type.

 

Product Details

 

Gene Symbol: BDB; BDB1; NTRKR2

 

NCBI Gene ID: 4920

 

Uniprot Entry: Q01974

 

Construct Details: Full length human ROR2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGCCCGGGGCTCGGCGCTCCCGCGGCGGCCGCTGCTGTGCATCCCGGCCGTCTGGGCGGCCGCCGCGCTTCTGCTCTC

AGTGTCCCGGACTTCAGGTGAAGTGGAGGTTCTGGATCCGAACGACCCTTTAGGACCCCTTGATGGGCAGGACGGCCCGA

TTCCAACTCTGAAAGGTTACTTTCTGAATTTTCTGGAGCCAGTAAACAATATCACCATTGTCCAAGGCCAGACGGCAATT

CTGCACTGCAAGGTGGCAGGAAACCCACCCCCTAACGTGCGGTGGCTAAAGAATGATGCCCCGGTGGTGCAGGAGCCGCG

GCGGATCATCATCCGGAAGACAGAATATGGTTCACGACTGCGAATCCAGGACCTGGACACGACAGACACTGGCTACTACC

AGTGCGTGGCCACCAACGGGATGAAGACCATTACCGCCACTGGCGTCCTGTTTGTGCGGCTGGGTCCAACGCACAGCCCA

AATCATAACTTTCAGGATGATTACCACGAGGATGGGTTCTGCCAGCCTTACCGGGGAATTGCCTGTGCACGCTTCATTGG

CAACCGGACCATTTATGTGGACTCGCTTCAGATGCAGGGGGAGATTGAAAACCGAATCACAGCGGCCTTCACCATGATCG

GCACGTCTACGCACCTGTCGGACCAGTGCTCACAGTTCGCCATCCCATCCTTCTGCCACTTCGTGTTTCCTCTGTGCGAC

GCGCGCTCCCGGACACCCAAGCCGCGTGAGCTGTGCCGCGACGAGTGCGAGGTGCTGGAGAGCGACCTGTGCCGCCAGGA

GTACACCATCGCCCGCTCCAACCCGCTCATCCTCATGCGGCTTCAGCTGCCCAAGTGTGAGGCGCTGCCCATGCCTGAGA

GCCCCGACGCTGCCAACTGCATGCGCATTGGCATCCCAGCCGAGAGGCTGGGCCGCTACCATCAGTGCTATAACGGCTCA

GGCATGGATTACAGAGGAACGGCAAGCACCACCAAGTCAGGCCACCAGTGCCAGCCGTGGGCCCTGCAGCACCCCCACAG

CCACCACCTGTCCAGCACAGACTTCCCTGAGCTTGGAGGGGGGCACGCCTACTGCCGGAACCCCGGAGGCCAGATGGAGG

GCCCCTGGTGCTTTACGCAGAATAAAAACGTACGCATGGAACTGTGTGACGTACCCTCGTGTAGTCCCCGAGACAGCAGC

AAGATGGGGATTCTGTACATCTTGGTCCCCAGCATCGCAATTCCACTGGTCATCGCTTGCCTTTTCTTCTTGGTTTGCAT

GTGCCGGAATAAGCAGAAGGCATCTGCGTCCACACCGCAGCGGCGACAGCTGATGGCCTCGCCCAGCCAAGACATGGAAA

TGCCCCTCATTAACCAGCACAAACAGGCCAAACTCAAAGAGATCAGCCTGTCTGCGGTGAGGTTCATGGAGGAGCTGGGA

GAGGACCGGTTTGGGAAAGTCTACAAAGGTCACCTGTTCGGCCCTGCCCCGGGGGAGCAGACCCAGGCTGTGGCCATCAA

AACGCTGAAGGACAAAGCGGAGGGGCCCCTGCGGGAGGAGTTCCGGCATGAGGCTATGCTGCGAGCACGGCTGCAACACC

CCAACGTCGTCTGCCTGCTGGGCGTGGTGACCAAGGACCAGCCCCTGAGCATGATCTTCAGCTACTGTTCGCACGGCGAC

CTCCACGAATTCCTGGTCATGCGCTCGCCGCACTCGGACGTGGGCAGCACCGATGATGACCGCACGGTGAAGTCCGCCCT

GGAGCCCCCCGACTTCGTGCACCTTGTGGCACAGATCGCGGCGGGGATGGAGTACCTATCCAGCCACCACGTGGTTCACA

AGGACCTGGCCACCCGCAATGTGCTAGTGTACGACAAGCTGAACGTGAAGATCTCAGACTTGGGCCTCTTCCGAGAGGTG

TATGCCGCCGATTACTACAAGCTGCTGGGGAACTCGCTGCTGCCTATCCGCTGGATGGCCCCAGAGGCCATCATGTACGG

CAAGTTCTCCATCGACTCAGACATCTGGTCCTACGGTGTGGTCCTGTGGGAGGTCTTCAGCTACGGCCTGCAGCCCTACT

GCGGGTACTCCAACCAGGATGTGGTGGAGATGATCCGGAACCGGCAGGTGCTGCCTTGCCCCGATGACTGTCCCGCCTGG

GTGTATGCCCTCATGATCGAGTGCTGGAACGAGTTCCCCAGCCGGCGGCCCCGCTTCAAGGACATCCACAGCCGGCTCCG

AGCCTGGGGCAACCTTTCCAACTACAACAGCTCGGCGCAGACCTCGGGGGCCAGCAACACCACGCAGACCAGCTCCCTGA

GCACCAGCCCAGTGAGCAATGTGAGCAACGCCCGCTACGTGGGGCCCAAGCAGAAGGCCCCGCCCTTCCCACAGCCCCAG

TTCATCCCCATGAAGGGCCAGATCAGACCCATGGTGCCCCCGCCGCAGCTCTACGTCCCCGTCAACGGCTACCAGCCGGT

GCCGGCCTATGGGGCCTACCTGCCCAACTTCTACCCGGTGCAGATCCCAATGCAGATGGCCCCGCAGCAGGTGCCTCCTC

AGATGGTCCCCAAGCCCAGCTCACACCACAGTGGCAGTGGCTCCACCAGCACAGGCTACGTCACCACGGCCCCCTCCAAC

ACATCCATGGCAGACAGGGCAGCCCTGCTCTCAGAGGGCGCTGATGACACACAGAACGCCCCAGAAGATGGGGCCCAGAG

CACCGTGCAGGAAGCAGAGGAGGAGGAGGAAGGCTCTGTCCCAGAGACTGAGCTGCTGGGGGACTGTGACACTCTGCAGG

TGGACGAGGCCCAAGTCCAGCTGGAAGCTTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 267:26181 (1992). 

2. Nat. Genet. 24:275 (2000).

3. Am. J. Hum. Genet. 67:822 (2000).

4. Nat. Genet. 25:419 (2000).

5. Nat. Genet. 25:423 (2000).

6. Mol. Endocrinol. 21:3050 (2007).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0229-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.