ASTN2 (Astrotactin-2) is a two-pass type I transmembrane protein that belongs to the astrotactin family.  ASTN2 contains 2 extracellular domains (ECD), including a 157 aa (amino acid) long small ECD1 and a 884 aa long large ECD2.  The ECD2 is composed of three EGF-like motifs and one Fibronectin type-III motif.  The C-terminal region after the fibronectin type-III domain presents structural similarity to annexin domains and binds calcium ions. ASTN2 is expressed in the brain and may function in neuronal migration, based on functional studies of the related ASTN1.  ASTN2 mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. ASTN2 also promotes ASTN1 internalization and intracellular transport of endocytosed ASTN1. ASTN2 binds inositol-4,5-bisphosphate, inositol-3,4,5-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, suggesting it is recruited to membranes lipids with a phosphoinositide headgroup. A deletion at the ASTN2 locus has been associated with schizophrenia.

ASTN2 (Astrotactin-2) is a two-pass type I transmembrane protein that belongs to the astrotactin family.  ASTN2 contains 2 extracellular domains (ECD), including a 157 aa (amino acid) long small ECD1 and a 884 aa long large ECD2.  The ECD2 is composed of three EGF-like motifs and one Fibronectin type-III motif.  The C-terminal region after the fibronectin type-III domain presents structural similarity to annexin domains and binds calcium ions. ASTN2 is expressed in the brain and may function in neuronal migration, based on functional studies of the related ASTN1.  ASTN2 mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. ASTN2 also promotes ASTN1 internalization and intracellular transport of endocytosed ASTN1. ASTN2 binds inositol-4,5-bisphosphate, inositol-3,4,5-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, suggesting it is recruited to membranes lipids with a phosphoinositide headgroup. A deletion at the ASTN2 locus has been associated with schizophrenia.

 

Product Details

 

Gene Symbol: ASTN2; Astrotactin-2

 

NCBI Gene ID: 23245

 

Uniprot Entry: O75129

 

Construct Details: Full length human ASTN2 gene (encoding Uniprot# O75129-2) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3867 (bp)

 

Gene Insert Sequence:  

ATGGCCGCCGCCGGCGCCCGGCTCAGCCCCGGCCCCGGCTCGGGGCTCCGGGGGCGGCCGAGGCTCTGCT
TCCACCCGGGGCCGCCGCCACTGCTGCCGCTGCTGCTGCTGTTCCTGCTCCTGCTGCCGCCGCCGCCGCT
GCTGGCCGGCGCCACCGCCGCTGCCTCGCGGGAGCCCGACAGCCCGTGCCGGCTGAAGACCGTCACGGTG
TCCACACTGCCCGCCCTGCGGGAGAGCGACATCGGCTGGAGCGGCGCCCGCGCCGGGGCCGGGGCTGGGA
CCGGGGCCGGAGCCGCCGCCGCCGCCGCGTCCCCGGGCTCTCCTGGCTCTGCCGGCACCGCCGCCGAGTC
GCGCCTCCTGCTCTTTGTGCGTAACGAGCTGCCGGGGCGCATCGCGGTGCAGGACGACCTGGACAACACC
GAGCTGCCCTTCTTCACCCTGGAGATGTCTGGCACAGCAGCGGACATCTCGCTGGTGCACTGGAGACAGC
AGTGGCTGGAGAATGGCACCTTGTACTTCCACGTCTCCATGAGCAGCTCCGGGCAGCTGGCCCAAGCCAC
CGCCCCCACTCTCCAGGAGCCCTCGGAGATTGTTGAGGAGCAGATGCACATCCTCCACATTTCTGTGATG
GGTGGCCTCATCGCGCTGCTGCTGCTGCTGCTGGTGTTCACCGTGGCGCTGTACGCCCAGCGACGTTGGC
AGAAGCGTCGCCGCATCCCCCAGAAGAGCGCAAGCACAGAAGCCACTCATGAGATCCACTACATCCCATC
TGTGCTGCTGGGTCCCCAGGCGCGGGAGAGCTTCCGTTCATCCCGGCTGCAAACCCACAATTCCGTCATT
GGCGTGCCCATCCGGGAGACTCCCATCCTGGATGACTATGACTGTGAGGAGGATGAGGAGCCACCTAGGC
GGGCCAACCATGTCTCCCGCGAGGACGAGTTTGGCAGCCAGGTGACCCACACTCTGGACAGTCTGGGACA
TCCAGGGGAAGAGAAGGTGGACTTTGAGAAGAAAGGAGGAATCAGCTTTGGGAGAGCCAAGGGGACGTCG
GGCTCAGAGGCAGACGATGAAACTCAGCTGACATTCTACACGGAGCAGTACCGCAGTCGCCGCCGCAGCA
AAGGTTTGCTGAAAAGCCCAGTGAACAAGACAGCCCTGACACTGATTGCTGTGAGTTCCTGCATCCTGGC
CATGGTGTGTGGCAGCCAGATGTCTTGTCCACTCACTGTGAAGGTGACTCTGCATGTGCCCGAGCACTTC
ATAGCAGATGGAAGCAGCTTCGTGGTGAGTGAAGGGAGCTACCTGGACATCTCCGACTGGTTAAACCCGG
CCAAGCTTTCCCTGTATTACCAGATCAATGCCACCTCCCCATGGGTGAGGGACCTCTGTGGACAAAGGAC
GACAGATGCCTGTGAGCAGCTCTGCGACCCAGAAACCGGAGAGTGCAGCTGTCATGAAGGCTATGCCCCT
GACCCTGTTCACAGACACCTGTGTGTGCGCAGTGACTGGGGACAGAGTGAAGGACCTTGGCCCTACACGA
CACTTGAGAGGGGCTATGATCTGGTGACAGGGGAGCAAGCCCCTGAAAAGATTCTCAGGTCTACTTTCAG
CTTGGGCCAAGGCCTCTGGCTTCCTGTCAGCAAAAGCTTTGTGGTTCCGCCTGTGGAGCTGTCCATCAAC
CCCCTGGCCAGCTGCAAGACCGATGTGCTCGTCACGGAAGACCCTGCAGATGTCAGGGAAGAAGCGATGC
TGTCCACATACTTTGAAACCATCAATGACCTGCTGTCTTCCTTCGGGCCAGTTCGTGACTGCTCTCGGAA
CAATGGGGGCTGCACTCGCAACTTCAAGTGTGTGTCTGACCGGCAGGTGGATTCCTCGGGATGTGTGTGC
CCTGAGGAGCTGAAACCCATGAAGGATGGCTCTGGCTGCTACGACCACTCCAAAGGCATTGACTGCTCTG
ATGGCTTTAATGGCGGCTGTGAGCAGCTGTGCCTGCAGCAGACGCTGCCCCTGCCCTACGATGCCACTTC
GAGCACCATCTTCATGTTCTGCGGTTGCGTGGAGGAGTACAAACTGGCTCCTGATGGAAAATCCTGCTTA
ATGCTCTCAGATGTCTGCGAGGGCCCCAAGTGCCTCAAACCTGACTCCAAATTCAATGATACCCTCTTTG
GAGAGATGCTACATGGTTACAACAACCGGACCCAGCATGTGAACCAAGGCCAAGTCTTCCAGATGACCTT
TAGGGAGAACAACTTCATCAAGGACTTTCCCCAGCTGGCCGATGGGCTGTTGGTGATCCCGCTGCCGGTG
GAGGAGCAGTGCCGGGGGGTCCTCTCCGAGCCCCTTCCAGACCTCCAACTGCTCACTGGAGATATCAGGT
ATGATGAGGCCATGGGTTACCCCATGGTGCAGCAGTGGCGGGTCCGGAGCAACCTCTACCGTGTGAAGCT
CAGCACCATCACCCTCGCAGCAGGCTTCACTAATGTTCTCAAGATCCTGACCAAGGAGAGCAGTCGGGAG
GAGCTGCTGTCCTTCATCCAGCACTATGGCTCCCACTACATCGCAGAGGCCCTCTATGGCTCAGAGCTCA
CCTGCATCATCCACTTTCCCAGCAAGAAGGTCCAGCAGCAGCTGTGGCTCCAGTATCAGAAAGAGACCAC
AGAGCTGGGCAGCAAGAAGGAGCTCAAGTCCATGCCCTTCATCACCTACCTCTCAGGTTTGCTGACAGCC
CAGATGCTGTCAGATGACCAGCTCATTTCAGGTGTGGAGATTCGCTGTGAGGAGAAGGGGCGCTGTCCAT
CTACCTGTCACCTTTGCCGCCGGCCAGGCAAGGAGCAGCTGAGCCCCACACCAGTGCTGCTGGAAATCAA
CCGTGTGGTGCCACTTTATACCCTCATCCAAGACAATGGCACAAAGGAGGCCTTCAAGAGTGCACTGATG
AGTTCCTACTGGTGCTCAGGGAAAGGGGATGTGATCGATGACTGGTGCAGGTGTGACCTCAGCGCCTTTG
ATGCCAATGGGCTCCCCAACTGCAGCCCCCTTCTGCAGCCGGTGCTGCGGCTGTCCCCAACAGTGGAGCC
CTCCAGTACTGTGGTCTCCTTGGAGTGGGTGGATGTTCAGCCAGCTATTGGGACCAAGGTCTCCGACTAT
ATTCTGCAGCATAAGAAAGTGGATGAATACACAGACACTGACCTGTACACAGGAGAATTCCTGAGTTTTG
CTGATGACTTACTCTCTGGCCTGGGCACATCTTGTGTAGCAGCTGGTCGAAGCCATGGAGAGGTCCCTGA
AGTCAGTATCTACTCAGTCATCTTCAAGTGTCTGGAGCCCGACGGTCTCTACAAGTTCACTCTGTATGCT
GTGGATACACGAGGGAGGCACTCAGAGCTAAGCACGGTGACCCTGAGGACGGCCTGTCCACTGGTAGATG
ACAACAAGGCAGAAGAAATAGCTGACAAGATCTACAATCTGTACAATGGGTACACAAGTGGAAAGGAGCA
GCAGATGGCCTACAACACACTGATGGAGGTCTCAGCCTCGATGCTGTTCCGAGTCCAGCACCACTACAAC
TCTCACTATGAAAAGTTTGGCGACTTCGTCTGGAGAAGTGAGGATGAGCTGGGGCCCAGGAAGGCCCACC
TGATTCTACGGCGACTGGAGAGGGTGAGTAGCCACTGCTCCAGCCTCCTGCGGAGTGCCTACATCCAGAG
CCGCGTGGAAACAGTGCCCTATCTTTTCTGCCGCAGCGAGGAGGTCCGGCCTGCAGGCATGGTGTGGTAT
AGCATCCTCAAGGACACCAAAATCACGTGTGAGGAGAAGATGGTGTCAATGGCCCGAAACACGTACGGGG
AGTCCAAGGGCCGGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Documentation

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.