EPHB2 (EPH receptor B2), is a single-pass type I transmembrane protein that belongs to the Ephrin receptor subfamily of the tyrosine protein kinase family. The EPH subfamily receptors bind transmembrane ligands Ephrins that are involved in diverse cellular processes, including motility, division, and differentiation. Both EPH receptors and Ephrin ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling.  EPHB2 belongs to the EPHB subgroup of the EPH subfamily that are distinguished from other members by sequence homology and preferential binding affinity to Ephrin-B ligands. EPHB2 comprises a large ligand-binding extracellular domain (ECD), a transmembrane region (TM), and an intracellular tyrosine kinase domain.  EPHB2 functions in axon guidance during development of contralateral inner ear efferent growth cones.  EPHB2 regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Forward and reverse signaling through Ephrin-B2/EPHB2 regulate movement and adhesion of cells. EPHB2 may also function as a tumor suppressor. Allelic variants of the EPHB2 gene are associated with prostate and brain cancer susceptibility.

EPHB2 (EPH receptor B2), is a single-pass type I transmembrane protein that belongs to the Ephrin receptor subfamily of the tyrosine protein kinase family. The EPH subfamily receptors bind transmembrane ligands Ephrins that are involved in diverse cellular processes, including motility, division, and differentiation. Both EPH receptors and Ephrin ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling.  EPHB2 belongs to the EPHB subgroup of the EPH subfamily that are distinguished from other members by sequence homology and preferential binding affinity to Ephrin-B ligands. EPHB2 comprises a large ligand-binding extracellular domain (ECD), a transmembrane region (TM), and an intracellular tyrosine kinase domain.  EPHB2 functions in axon guidance during development of contralateral inner ear efferent growth cones.  EPHB2 regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Forward and reverse signaling through Ephrin-B2/EPHB2 regulate movement and adhesion of cells. EPHB2 may also function as a tumor suppressor. Allelic variants of the EPHB2 gene are associated with prostate and brain cancer susceptibility.

 

Product Details

 

Gene Symbol: DRT; EPHT3; EK5; ERK; CAPB; Hek5; PCBC; EPTH3; Tyro5; BDPLT22

 

NCBI Gene ID: 2048

 

Uniprot Entry: P29323

 

Construct Details: The recombinant human EPHB2-Fc fusion protein is expressed as a 742 amino acid protein consisting of Val19 - Glu532 region of EPHB2 and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

   

Source: Human cells stably expressing EPHB2-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
VEETLMDSTT ATAELGWMVH PPSGWEEVSG YDENMNTIRT YQVCNVFESS QNNWLRTKFI

        70         80         90        100        110        120
RRRGAHRIHV EMKFSVRDCS SIPSVPGSCK ETFNLYYYEA DFDSATKTFP NWMENPWVKV

       130        140        150        160        170        180
DTIAADESFS QVDLGGRVMK INTEVRSFGP VSRSGFYLAF QDYGGCMSLI AVRVFYRKCP

       190        200        210        220        230        240
RIIQNGAIFQ ETLSGAESTS LVAARGSCIA NAEEVDVPIK LYCNGDGEWL VPIGRCMCKA

       250        260        270        280        290        300
GFEAVENGTV CRGCPSGTFK ANQGDEACTH CPINSRTTSE GATNCVCRNG YYRADLDPLD

       310        320        330        340        350        360
MPCTTIPSAP QAVISSVNET SLMLEWTPPR DSGGREDLVY NIICKSCGSG RGACTRCGDN

       370        380        390        400        410        420
VQYAPRQLGL TEPRIYISDL LAHTQYTFEI QAVNGVTDQS PFSPQFASVN ITTNQAAPSA

       430        440        450        460        470        480
VSIMHQVSRT VDSITLSWSQ PDQPNGVILD YELQYYEKEL SEYNATAIKS PTNTVTVQGL

       490        500        510        520        530        540
KAGAIYVFQV RARTVAGYGR YSGKMYFQTM TEAESTGTHT CPPCPAPELL GGPSVFLFPP

       550        560        570        580        590        600
KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV

       610        620        630        640        650        660
LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL

       670        680        690        700        710        720
TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC

       730        740
SVMHEALHNH YTQKSLSLSP GK

 

M.W.: Calculated molecular mass 82.4 kDa; estimated by SDS-PAGE under reducing condition: ~85-90 kDa 

 

Calculated PI: 5.69

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  115305

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.