Angiotensin-converting enzymes including ACE and ACE2 are a central component of the renin-angiotensin system (RAS) that controls blood pressure by regulating the volume of fluids in the body. Both ACE & ACE2 are single-pass type I membrane proteins that belong to the peptidase M2 family.  Unlike ACE, the extracellular region of ACE2 contains only 1 zinc-binding metallopeptidase domain (19-611aa) and a C-terminal domain (612-740aa) that is 48% identical to human collectrin.  Its cytoplasmic tail contains several motifs such as LIR, PDZ-binding, PTB and endocytic sorting signals allowing interactions with proteins that mediate endocytic trafficking and autophagy.  The secreted ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is expressed in various human organs and may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, ACE2 is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). 

Angiotensin-converting enzymes including ACE and ACE2 are a central component of the renin-angiotensin system (RAS) that controls blood pressure by regulating the volume of fluids in the body. Both ACE & ACE2 are single-pass type I membrane proteins that belong to the peptidase M2 family.  Unlike ACE, the extracellular region of ACE2 contains only 1 zinc-binding metallopeptidase domain (19-611aa) and a C-terminal domain (612-740aa) that is 48% identical to human collectrin.  Its cytoplasmic tail contains several motifs such as LIR, PDZ-binding, PTB and endocytic sorting signals allowing interactions with proteins that mediate endocytic trafficking and autophagy.  The secreted ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is expressed in various human organs and may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, ACE2 is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). 

 

Product Details

 

Gene Symbol: ACE2; ACEH

 

NCBI Gene ID: 59272

 

Uniprot Entry: Q9BYF1

 

Construct Details: The recombinant human ACE2 ECDc is expressed as a 606 amino acid protein consisting of Gln18 - Pro612 region (metallopeptidase/catalytic domain) of ACE2 and a C-terminal His-tag. 

 

Source: Human cells stably expressing ACE2 ECDc (extracellular catalytic domain) and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS 

        70         80         90        100        110        120 

TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN 

       130        140        150        160        170        180 

PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE 

       190        200        210        220        230        240 

DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS 

       250        260        270        280        290        300 

PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS 

       310        320        330        340        350        360 

VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG 

       370        380        390        400        410        420 

HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN 

       430        440        450        460        470        480 

FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY 

       490        500        510        520        530        540 

CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM 

       550        560        570        580        590        600 

LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPSTGHH 

 

HHHHHH 

 

  

M.W.: Calculated molecular mass (kDa): 70.2; Estimated by SDS-PAGE under reducing condition (kDa): ~75-80

 

Calculated PI: 5.32

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  150730

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.