LSR (lipolysis stimulated lipoprotein receptor), also known as angulin-1, ILDR3 (immunoglobulin (Ig)-like domain containing receptor 3) and LISCH/LISCH7, belongs to the LISCH7 family of the Ig superfamily (IgSF).  LSR is a single-pass type I transmembrane proteins with an extracellular domain containing an Ig-like V-type motif, a transmembrane domain (TM), and a cytoplasmic tail enriched with basic/acidic/polar residues.  LSR is located in plasma membrane and tight junction.  LSR may be involved in establishment of skin barrier; protein localization to tricellular tight junction; and tricellular tight junction assembly. It is predicted to act upstream of or within maintenance of blood-brain barrier.  LSR maintains epithelial barrier function by recruiting MARVELD2/tricellulin to tricellular tight junctions.  LSR also binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells. LSR may play a role in the clearance of triglyceride-rich lipoprotein from blood. Defects in LSR may be the cause of familial intrahepatic cholestasis.  

LSR (lipolysis stimulated lipoprotein receptor), also known as angulin-1, ILDR3 (immunoglobulin (Ig)-like domain containing receptor 3) and LISCH/LISCH7, belongs to the LISCH7 family of the Ig superfamily (IgSF).  LSR is a single-pass type I transmembrane proteins with an extracellular domain containing an Ig-like V-type motif, a transmembrane domain (TM), and a cytoplasmic tail enriched with basic/acidic/polar residues.  LSR is located in plasma membrane and tight junction.  LSR may be involved in establishment of skin barrier; protein localization to tricellular tight junction; and tricellular tight junction assembly. It is predicted to act upstream of or within maintenance of blood-brain barrier.  LSR maintains epithelial barrier function by recruiting MARVELD2/tricellulin to tricellular tight junctions.  LSR also binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells. LSR may play a role in the clearance of triglyceride-rich lipoprotein from blood. Defects in LSR may be the cause of familial intrahepatic cholestasis.  

 

Product Details

 

Gene Symbol: LSR; ILDR3; LISCH7; LISCH; Angulin-1

 

NCBI Gene ID: 51599

 

Uniprot Entry: Q86X29

 

Construct Details: The recombinant human LSR-Fc is expressed as a 384-amino acid protein consisting of  Gln91 -  Ala246 region of LSR and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source: Human cells stably expressing LSR-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QVTVSNPYHV VILFQPVTLP CTYQMTSTPT QPIVIWKYKS FCRDRIADAF SPASVDNQLN  

        70         80         90        100        110        120 

AQLAAGNPGY NPYVECQDSV RTVRVVATKQ GNAVTLGDYY QGRRITITGN ADLTFDQTAW  

       130        140        150        160        170        180 

GDSGVYYCSV VSAQDLQGNN EAYAELIVLG RTSGVASSGT HTCPPCPAPE LLGGPSVFLF  

       190        200        210        220        230        240 

PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV  

       250        260        270        280        290        300 

SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV  

       310        320        330        340        350        360 

SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF  

       370        380 

SCSVMHEALH NHYTQKSLSL SPGK 

 

 

  

M.W.: Calculated molecular mass (kDa): 42.6; Estimated by SDS-PAGE under reducing condition (kDa): ~45

 

Calculated PI: 6.49

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  61935

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.