LILRA3 (Leukocyte immunoglobulin like receptor A3), also known as CD85e and LIR4 (Leukocyte immunoglobulin-like receptor 4), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF). LIR family members are known to have activating and inhibitory functions in leukocytes. LILRA3 belongs to the subfamily A class of LIR receptors (LILRAs), which are single-pass type I transmembrane proteins with 2-4 extracellular Ig-like domains, a transmembrane domain (TM), and a short cytoplasmic tail. LILRA3 contains 4 Ig-like C2 type domains in the extracellular region. LILRA3 acts as a receptor for class I MHC antigens. LILRA3 binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. LILRA3 binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.
Gene Symbol: LILRA3; LIR4; CD85E; LIR-4; ILT6; ILT-6; HM31; HM43
NCBI Gene ID: 11026
Uniprot Entry: Q8N6C8
Construct Details: The recombinant human LILRA3 ECD protein is expressed as an 644-amino acid protein consisting of Gly24 - Glu439 region of LILRA3 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions. LILRA3 ECD contains 5 potential N-linked glycosylation sites.
Source: Human cells stably expressing LILRA3-Fc and growing in chemical-defined media with no animal component or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
GPLPKPTLWA EPGSVITQGS PVTLRCQGSL ETQEYHLYRE KKTALWITRI PQELVKKGQF
70 80 90 100 110 120
PILSITWEHA GRYCCIYGSH TAGLSESSDP LELVVTGAYS KPTLSALPSP VVTSGGNVTI
130 140 150 160 170 180
QCDSQVAFDG FILCKEGEDE HPQCLNSHSH ARGSSRAIFS VGPVSPSRRW SYRCYGYDSR
190 200 210 220 230 240
APYVWSLPSD LLGLLVPGVS KKPSLSVQPG PVVAPGEKLT FQCGSDAGYD RFVLYKEWGR
250 260 270 280 290 300
DFLQRPGRQP QAGLSQANFT LGPVSRSYGG QYTCSGAYNL SSEWSAPSDP LDILITGQIR
310 320 330 340 350 360
ARPFLSVRPG PTVASGENVT LLCQSQGGMH TFLLTKEGAA DSPLRLKSKR QSHKYQAEFP
370 380 390 400 410 420
MSPVTSAHAG TYRCYGSLSS NPYLLTHPSD PLELVVSGAA ETLSPPQNKS DSKAGESTGT
430 440 450 460 470 480
HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE
490 500 510 520 530 540
VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP
550 560 570 580 590 600
REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS
610 620 630 640
FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK
M.W.: Calculated molecular mass (kDa): 70.6; Estimated by SDS-PAGE under reducing condition (kDa): ~85-90
Calculated PI: 8.20
Calculated extinction coefficients (M-1 cm-1, at 280nm): 101730
Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")
Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity: Not available
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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