Human IL1RL1/ST2/IL1R4/IL33R Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Product ID: pLTC-IL1RL1 (SKU#: LTP1240)

Price:

$996.00

Description

IL1RL1 (interleukin 1 receptor-like 1), also known as ST2, DER4, IL1R4 and IL33R, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin 1 receptor (IL1R) family. The IL1R family comprises at least 10 members, which typically contain 3 immunoglobulin (Ig)-like domains in the extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. IL1RL1 can be induced by proinflammatory stimuli, and is involved in the function of helper T (Th) cells. IL1RL1, IL1R1, IL1R2 and IL1RL2 form a cytokine receptor gene cluster in chromosome 2q12. IL1RL1 is expressed on mast cells, activated Th2 cells, macrophages, and cardiac myocytes. It was identified as the receptor for IL33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts. IL1RL1 may promote mast cell and Th2 dependent inflammation, enhance antigen-induced hypernociception and protects from atherosclerosis and cardiac hypertrophy. IL1RL1 has been used as a marker for the diagnosis and prognosis of cardiovascular diseases. Mutations in the IL1RL1 gene are associated with blood eosinophil counts and with asthma. A soluble IL1RL1 isoform is released by activated Th2 cells and is elevated in the serum in allergic asthma. Soluble IL1RL1 may function as a decoy receptor that blocks IL33/IL1RL1-mediated signaling activity.

Product Details

Gene Symbol: IL1RL1; ST2; IL1R4; T1; DER4; ST2L; ST2V; FIT-1; IL33R

NCBI Gene ID: 9173

Uniprot Entry: Q01638

Construct Details: Full length human IL1RL1/ST2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC or pLTC-Marker (see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Sequence:

ATGGGGTTTTGGATCTTAGCAATTCTCACAATTCTCATGTATTCCACAGCAGCAAAGTTTAGTAAACAATCATGGGGCCT

GGAAAATGAGGCTTTAATTGTAAGATGTCCTAGACAAGGAAAACCTAGTTACACCGTGGATTGGTATTACTCACAAACAA

ACAAAAGTATTCCCACTCAGGAAAGAAATCGTGTGTTTGCCTCAGGCCAACTTCTGAAGTTTCTACCAGCTGCAGTTGCT

GATTCTGGTATTTATACCTGTATTGTCAGAAGTCCCACATTCAATAGGACTGGATATGCGAATGTCACCATATATAAAAA

ACAATCAGATTGCAATGTTCCAGATTATTTGATGTATTCAACAGTATCTGGATCAGAAAAAAATTCCAAAATTTATTGTC

CTACCATTGACCTCTACAACTGGACAGCACCTCTTGAGTGGTTTAAGAATTGTCAGGCTCTTCAAGGATCAAGGTACAGG

GCGCACAAGTCATTTTTGGTCATTGATAATGTGATGACTGAGGACGCAGGTGATTACACCTGTAAATTTATACACAATGA

AAATGGAGCCAATTATAGTGTGACGGCGACCAGGTCCTTCACGGTCAAGGATGAGCAAGGCTTTTCTCTGTTTCCAGTAA

TCGGAGCCCCTGCACAAAATGAAATAAAGGAAGTGGAAATTGGAAAAAACGCAAACCTAACTTGCTCTGCTTGTTTTGGA

AAAGGCACTCAGTTCTTGGCTGCCGTCCTGTGGCAGCTTAATGGAACAAAAATTACAGACTTTGGTGAACCAAGAATTCA

ACAAGAGGAAGGGCAAAATCAAAGTTTCAGCAATGGGCTGGCTTGTCTAGACATGGTTTTAAGAATAGCTGACGTGAAGG

AAGAGGATTTATTGCTGCAGTACGACTGTCTGGCCCTGAATTTGCATGGCTTGAGAAGGCACACCGTAAGACTAAGTAGG

AAAAATCCAATTGATCATCATAGCATCTACTGCATAATTGCAGTATGTAGTGTATTTTTAATGCTAATCAATGTCCTGGT

TATCATCCTAAAAATGTTCTGGATTGAGGCCACTCTGCTCTGGAGAGACATAGCTAAACCTTACAAGACTAGGAATGATG

GAAAGCTCTATGATGCTTATGTTGTCTACCCACGGAACTACAAATCCAGTACAGATGGGGCCAGTCGTGTAGAGCACTTT

GTTCACCAGATTCTGCCTGATGTTCTTGAAAATAAATGTGGCTATACCTTATGCATTTATGGGAGAGATATGCTACCTGG

AGAAGATGTAGTCACTGCAGTGGAAACCAACATACGAAAGAGCAGGCGGCACATTTTCATCCTGACCCCTCAGATCACTC

ACAATAAGGAGTTTGCCTACGAGCAGGAGGTTGCCCTGCACTGTGCCCTCATCCAGAACGACGCCAAGGTGATACTTATT

GAGATGGAGGCTCTGAGCGAGCTGGACATGCTGCAGGCTGAGGCGCTTCAGGACTCCCTCCAGCATCTTATGAAAGTACA

GGGGACCATCAAGTGGAGGGAGGACCACATTGCCAATAAAAGGTCCCTGAATTCTAAATTCTGGAAGCACGTGAGGTACC

AAATGCCTGTGCCAAGCAAAATTCCCAGAAAGGCCTCTAGTTTGACTCCCTTGGCTGCCCAGAAGCAATAG

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References