CCRL2 (C-C chemokine receptor-like 2) is a 7-transmembrane protein of the G protein-coupled receptor 1 (GPCR1) family and most closely related to CCR1. CCRL2 acts as a receptor for C-C type chemokines. Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. The CCRL2 gene and other chemokine receptor genes, including CCR1, CCR2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. CCRL2 is expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. The function of the CCRL2 gene is unknown.  It may act as a receptor for CCL19 and chemerin/RARRES2. However CCRL2 does not appear to be a signaling receptor.  It may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. CCRL2 may play a critical role in the development of Th2 responses.

CCRL2 (C-C chemokine receptor-like 2) is a 7-transmembrane protein of the G protein-coupled receptor 1 (GPCR1) family and most closely related to CCR1. CCRL2 acts as a receptor for C-C type chemokines. Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. The CCRL2 gene and other chemokine receptor genes, including CCR1, CCR2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. CCRL2 is expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. The function of the CCRL2 gene is unknown.  It may act as a receptor for CCL19 and chemerin/RARRES2. However CCRL2 does not appear to be a signaling receptor.  It may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. CCRL2 may play a critical role in the development of Th2 responses.

 

Product Details

 

Gene Symbol: CCRL2; CCR11; HCR; CKRX; CRAM; ACKR5; CRAM-A; CRAM-B

 

NCBI Gene ID: 9034

 

Uniprot Entry: O00421

 

Construct Details: Full length human CCRL2/CCR11 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1035 (bp)

 

Gene Insert Sequence:  

ATGGCCAATTACACGCTGGCACCAGAGGATGAATATGATGTCCTCATAGAAGGTGAACTGGAGAGCGATG

AGGCAGAGCAATGTGACAAGTATGACGCCCAGGCACTCTCAGCCCAGCTGGTGCCATCACTCTGCTCTGC

TGTGTTTGTGATCGGTGTCCTGGACAATCTCCTGGTTGTGCTTATCCTGGTAAAATATAAAGGACTCAAA

CGCGTGGAAAATATCTATCTTCTAAACTTGGCAGTTTCTAACTTGTGTTTCTTGCTTACCCTGCCCTTCT

GGGCTCATGCTGGGGGCGATCCCATGTGTAAAATTCTCATTGGACTGTACTTCGTGGGCCTGTACAGTGA

GACATTTTTCAATTGCCTTCTGACTGTGCAAAGGTACCTAGTGTTTTTGCACAAGGGAAACTTTTTCTCA

GCCAGGAGGAGGGTGCCCTGTGGCATCATTACAAGTGTCCTGGCATGGGTAACAGCCATTCTGGCCACTT

TGCCTGAATTCGTGGTTTATAAACCTCAGATGGAAGACCAGAAATACAAGTGTGCATTTAGCAGAACTCC

CTTCCTGCCAGCTGATGAGACATTCTGGAAGCATTTTCTGACTTTAAAAATGAACATTTCGGTTCTTGTC

CTCCCCCTATTTATTTTTACATTTCTCTATGTGCAAATGAGAAAAACACTAAGGTTCAGGGAGCAGAGGT

ATAGCCTTTTCAAGCTTGTTTTTGCCATAATGGTAGTCTTCCTTCTGATGTGGGCGCCCTACAATATTGC

ATTTTTCCTGTCCACTTTCAAAGAACACTTCTCCCTGAGTGACTGCAAGAGCAGCTACAATCTGGACAAA

AGTGTTCACATCACTAAACTCATCGCCACCACCCACTGCTGCATCAACCCTCTCCTGTATGCGTTTCTTG

ATGGGACATTTAGCAAATACCTCTGCCGCTGTTTCCATCTGCGTAGTAACACCCCACTTCAACCCAGGGG

GCAGTCTGCACAAGGCACATCGAGGGAAGAACCTGACCATTCCACCGAAGTGTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.