CFHR5 (Complement factor H-related protein 1) is a secreted glycoprotein with 9 conserved Sushi (CCP/SCR) domains in the extracellular region. Sushi is a motif of ~60 amino acids characterized by a framework of 4 conserved half-cystine residues (1-3 and 2-4 disulfide-bonded) and several other highly conserved residues including proline, tryptophan, tyrosine/phenylalanine and cysteine. This type of structure, also designated SCR (Short Consensus Repeat), is predominantly present in proteins associated with the complement system. CFHR5 is also a member of the complement factor H (CFH) gene family, and encodes one of the 5 CFH-related (CFHR) proteins. These 5 genes are closely linked to the CFH gene on chromosome 1q31-q32. The CFHRs are secreted plasma proteins synthesized primarily by the liver and composed of highly-related SCRs. Mutations in CFHR genes have been associated with dense deposit disease and atypical haemolytic-uraemic syndrome.  CFHR5 may be involved in complement regulation. CFHR5 forms head-to-tail homodimer and heterodimer with CFHR1 or CFHR2.  The first two SCRs in CFHR5 have heparin binding properties, a region within repeats 5-7 has heparin binding and C reactive protein binding properties, and the C-terminal SCRs are similar to a complement component 3 b (C3b) binding domain.  The dimerized forms of CFHR5 have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Allelic variations in the CFHR5 gene have been associated with 2 different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).

CFHR5 (Complement factor H-related protein 1) is a secreted glycoprotein with 9 conserved Sushi (CCP/SCR) domains in the extracellular region. Sushi is a motif of ~60 amino acids characterized by a framework of 4 conserved half-cystine residues (1-3 and 2-4 disulfide-bonded) and several other highly conserved residues including proline, tryptophan, tyrosine/phenylalanine and cysteine. This type of structure, also designated SCR (Short Consensus Repeat), is predominantly present in proteins associated with the complement system. CFHR5 is also a member of the complement factor H (CFH) gene family, and encodes one of the 5 CFH-related (CFHR) proteins. These 5 genes are closely linked to the CFH gene on chromosome 1q31-q32. The CFHRs are secreted plasma proteins synthesized primarily by the liver and composed of highly-related SCRs. Mutations in CFHR genes have been associated with dense deposit disease and atypical haemolytic-uraemic syndrome.  CFHR5 may be involved in complement regulation. CFHR5 forms head-to-tail homodimer and heterodimer with CFHR1 or CFHR2.  The first two SCRs in CFHR5 have heparin binding properties, a region within repeats 5-7 has heparin binding and C reactive protein binding properties, and the C-terminal SCRs are similar to a complement component 3 b (C3b) binding domain.  The dimerized forms of CFHR5 have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Allelic variations in the CFHR5 gene have been associated with 2 different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).

 

Product Details

 

Gene Symbol: CFHR5; FHR5; CFHL5; FHR-5; CFHR5D

 

NCBI Gene ID: 81494

 

Uniprot Entry: Q9BXR6

 

Construct Details: Full length human CFHR5 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1710 (bp)

 

Gene Insert Sequence:  

ATGTTGCTCTTATTCAGTGTAATCCTAATCTCATGGGTATCCACTGTTGGGGGAGAAGGAACACTTTGTG

ATTTTCCAAAAATACACCATGGATTTCTGTATGATGAAGAAGATTATAACCCTTTTTCCCAAGTTCCTAC

AGGGGAAGTTTTCTATTACTCCTGTGAATATAATTTTGTGTCTCCTTCAAAATCCTTTTGGACTCGCATA

ACATGCACAGAAGAAGGATGGTCACCAACACCGAAGTGTCTCAGAATGTGTTCCTTTCCTTTTGTGAAAA

ATGGTCATTCTGAATCTTCAGGACTAATACATCTGGAAGGTGATACTGTACAAATTATTTGCAACACAGG

ATACAGCCTTCAAAACAATGAGAAAAACATTTCGTGTGTAGAACGGGGCTGGTCCACTCCTCCCATATGC

AGCTTCACTAAAGGAGAATGTCATGTTCCAATTTTAGAAGCCAATGTAGATGCTCAGCCAAAAAAAGAAA

GCTACAAAGTTGGAGACGTGTTGAAATTCTCCTGCAGAAAAAATCTTATAAGAGTTGGATCAGACTCAGT

TCAATGTTACCAATTTGGGTGGTCACCTAACTTTCCAACATGCAAAGGACAAGTACGATCATGTGGTCCA

CCTCCTCAACTCTCCAATGGTGAAGTTAAGGAGATAAGAAAAGAGGAATATGGACACAATGAAGTAGTGG

AATATGATTGCAATCCTAATTTTATAATAAACGGGCCTAAGAAAATACAATGTGTGGATGGAGAATGGAC

AACTTTACCCACTTGTGTTGAACAAGTGAAAACATGTGGATACATACCTGAACTCGAGTACGGTTATGTT

CAGCCGTCTGTCCCTCCCTATCAACATGGAGTTTCAGTCGAGGTGAATTGCAGAAATGAATATGCAATGA

TTGGAAATAACATGATTACCTGTATTAATGGAATATGGACAGAGCTTCCTATGTGTGTTGCAACACACCA

ACTTAAGAGGTGCAAAATAGCAGGAGTTAATATAAAAACATTACTCAAGCTATCTGGGAAAGAATTTAAT

CATAATTCTAGAATACGTTACAGATGTTCAGACATCTTCAGATACAGGCACTCAGTCTGTATAAACGGGA

AATGGAATCCTGAAGTAGACTGCACAGAAAAAAGGGAACAATTCTGCCCACCGCCACCTCAGATACCTAA

TGCTCAGAATATGACAACCACAGTGAATTATCAGGATGGAGAAAAAGTAGCTGTTCTCTGTAAAGAAAAC

TATCTACTTCCAGAAGCAAAAGAAATTGTATGTAAAGATGGACGATGGCAATCATTACCACGCTGTGTTG

AGTCTACTGCATATTGTGGGCCCCCTCCATCTATTAACAATGGAGATACCACCTCATTCCCATTATCAGT

ATATCCTCCAGGGTCAACAGTGACGTACCGTTGCCAGTCCTTCTATAAACTCCAGGGCTCTGTAACTGTA

ACATGCAGAAATAAACAGTGGTCAGAACCACCAAGATGCCTAGATCCATGTGTGGTATCTGAAGAAAACA

TGAACAAAAATAACATACAGTTAAAATGGAGAAACGATGGAAAACTCTATGCAAAAACAGGGGATGCTGT

TGAATTCCAGTGTAAATTCCCACATAAAGCGATGATATCATCACCACCATTTCGAGCAATCTGTCAGGAA

GGGAAATTTGAATATCCTATATGTGAATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.