ACKR2 (atypical chemokine receptor 2) is a 7-transmembrane protein of the ACKR subfamily of the G protein-coupled receptor 1 (GPCR1) family.  ACKR controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. ACKR is also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. ACKR2 acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, ACKR2 activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1). Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, ACKR2 plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. ACKR2 plays a major role in the immune silencing of macrophages during the resolution of inflammation. ACKR2 also acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.

ACKR2 (atypical chemokine receptor 2) is a 7-transmembrane protein of the ACKR subfamily of the G protein-coupled receptor 1 (GPCR1) family.  ACKR controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. ACKR is also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. ACKR2 acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, ACKR2 activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1). Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, ACKR2 plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. ACKR2 plays a major role in the immune silencing of macrophages during the resolution of inflammation. ACKR2 also acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.

 

Product Details

 

Gene Symbol: ACKR2; D6; hD6; CCR9; CCBP2; CCR10; CMKBR9

 

NCBI Gene ID: 1238

 

Uniprot Entry: O00590

 

Construct Details: Full length human ACKR2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1155 (bp)

 

Gene Insert Sequence:  

ATGGCCGCCACTGCCTCTCCGCAGCCACTCGCCACTGAGGATGCCGATTCTGAGAATAGCAGCTTCTATT

ACTATGACTACCTGGATGAAGTGGCCTTCATGCTCTGCAGGAAGGATGCAGTGGTGTCCTTTGGCAAAGT

CTTCCTCCCAGTCTTCTATAGCCTGATTTTTGTGTTGGGCCTCAGCGGGAACCTCCTTCTTCTCATGGTC

TTGCTCCGTTACGTGCCTCGCAGGCGGATGGTTGAGATCTATCTGCTGAATCTGGCCATCTCCAACCTTC

TGTTTCTGGTGACACTGCCCTTCTGGGGCATCTCCGTGGCCTGGCATTGGGTCTTCGGGAGTTTCTTGTG

CAAGATGGTGAGCACTCTTTATACTATTAACTTTTACAGTGGCATCTTTTTCATTAGCTGCATGAGCCTG

GACAAGTACCTGGAGATCGTTCATGCTCAGCCCTACCACAGGCTGAGGACCCGGGCCAAGAGCCTGCTCC

TTGCTACCATAGTATGGGCTGTGTCCCTGGCCGTCTCCATCCCTGATATGGTCTTTGTACAGACACATGA

AAATCCCAAGGGTGTGTGGAACTGCCACGCAGATTTCGGCGGGCATGGGACCATTTGGAAGCTCTTCCTC

CGCTTCCAGCAGAACCTCCTAGGGTTTCTCCTTCCACTCCTTGCCATGATCTTCTTCTACTCCCGTATTG

GTTGTGTCTTGGTGAGGCTGAGGCCCGCAGGCCAGGGCCGGGCTTTAAAAATAGCTGCAGCCTTGGTGGT

GGCCTTCTTCGTGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGACCTGCAAGTA

TTCGGGAACTGTGAGGTCAGCCAGCATCTAGACTACGCACTCCAGGTAACAGAGAGCATCGCCTTCCTTC

ACTGCTGCTTTTCCCCCATCCTGTATGCCTTCTCCAGTCACCGCTTCCGCCAGTACCTGAAGGCTTTCCT

GGCTGCCGTGCTTGGATGGCACCTGGCACCTGGCACTGCCCAGGCCTCATTATCCAGCTGTTCTGAGAGC

AGCATACTTACTGCCCAAGAGGAAATGACTGGCATGAATGACCTTGGAGAGAGGCAGTCTGAGAACTACC

CTAACAAGGAGGATGTGGGGAATAAATCAGCCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.