ART4 or CD297 is a GPI (glycosylphosphatidylinositol)-linked NAD(P)(+)-arginine ADP-ribosyltransferase. ART4 contains a mono-ADP-ribosylation (ART) motif that may catalyze the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. The amino acid sequence consists of predominantly hydrophobic N- and C-terminal regions, which is characteristic of GPI-anchored proteins. Antigens of the Dombrock blood group system are located on the ART4 gene product, which is GPI-anchored to the erythrocyte membrane. Allelic variants may lead to adverse transfusion reactions.

ART4 or CD297 is a GPI (glycosylphosphatidylinositol)-linked NAD(P)(+)-arginine ADP-ribosyltransferase. ART4 contains a mono-ADP-ribosylation (ART) motif that may catalyze the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. The amino acid sequence consists of predominantly hydrophobic N- and C-terminal regions, which is characteristic of GPI-anchored proteins. Antigens of the Dombrock blood group system are located on the ART4 gene product, which is GPI-anchored to the erythrocyte membrane. Allelic variants may lead to adverse transfusion reactions.

 

Product Details

 

Gene Symbol: ART4; CD297; DO; DOK1; ARTC4

 

NCBI Gene ID: 420

 

Uniprot Entry: Q93070

 

Construct Details: Full length human ART4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 945 (bp)

 

Gene Insert Sequence:  

ATGGGTCCATTGATCAACAGATGCAAGAAGATTCTTCTCCCAACTACTGTACCTCCTGCAACGATGAGAA

TCTGGCTCCTTGGAGGCCTGCTGCCATTCCTGCTGCTCCTCTCTGGCCTGCAGAGACCCACAGAGGGTTC

TGAGGTTGCAATTAAAATCGACTTCGACTTCGCACCAGGTTCTTTTGATGATCAGTACCAAGGCTGTAGC

AAACAGGTTATGGAGAAACTAACTCAAGGGGATTATTTCACAAAAGACATAGAAGCCCAGAAGAATTATT

TTAGGATGTGGCAAAAAGCCCACTTAGCCTGGCTTAACCAAGGAAAAGTTCTACCCCAGAACATGACTAC

CACACACGCTGTGGCTATTTTGTTTTATACATTGAACAGCAATGTTCATTCTGACTTTACTAGAGCCATG

GCCTCTGTTGCCAGGACTCCACAGCAGTATGAACGTTCATTCCACTTCAAATATTTACACTACTACCTCA

CCTCAGCAATCCAGCTGCTGAGGAAAGACAGCATCATGGAGAATGGCACTCTGTGCTATGAGGTGCATTA

TAGGACGAAGGATGTCCACTTTAATGCCTACACAGGGGCCACCATTCGATTTGGCCAATTCCTCTCCACA

TCCCTCCTGAAAGAAGAGGCACAGGAGTTTGGGAACCAGACACTATTTACCATATTCACCTGCCTGGGTG

CACCTGTACAGTACTTCTCCCTCAAGAAGGAAGTCTTGATCCCTCCCTATGAGCTGTTTAAAGTTATAAA

TATGAGCTACCACCCAAGAGGAGACTGGTTGCAGTTGAGGTCAACTGGGAACCTGAGCACATATAACTGT

CAGCTGCTAAAAGCTTCCAGCAAGAAATGCATCCCTGATCCTATAGCTATTGCATCTCTCTCCTTTTTGA

CCAGTGTCATCATCTTTTCCAAAAGCAGAGTATAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.