There are 3 classes of receptors for the Fc region of IgG (FcγRs): FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), each with multiple isoforms. All these receptors are members of the Ig superfamily (IgSF) and they play important roles in the activation or inhibition of immune responses. FcγRI is a high-affinity receptor for IgG while both FcγRII and FcγRIII are low-affinity receptors that bind IgG in the form of immune complexes. There are two genes for human FcγRIII: A (CD16A) that encodes a single pass transmembrane receptor and B (CD16B) that encodes a GPI-anchored protein. The extracellular domains (ECDs) of CD16A and CD16B share 97% amino acid sequence homology. CD16A is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells. Signaling through CD16A results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells as well as clearance of immune complexes. In humans, a single nucleotide polymorphism creates high Fc-binding (176V) and low Fc-binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. CD16A surface expression requires interaction of an accessory chain, either the common gamma-chain or CD3zeta. A soluble form of CD16A can be produced by proteolytic cleavage and circulates in plasma, which is increased in rheumatoid arthritis and in coronary artery diseases.
Gene Symbol: CD16A; FCGR3A; FCG3; CD16; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
NCBI Gene ID: 2214
Uniprot Entry: P08637
Construct Details: The recombinant human CD16A-Fc is expressed as a 420-amino acid protein consisting of Gly17 - Gln208 region of CD16A-F176 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source: Human cells stably expressing human CD16A-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
GMRTEDLPKA VVFLEPQWYR VLEKDSVTLK CQGAYSPEDN STQWFHNESL ISSQASSYFI
70 80 90 100 110 120
DAATVDDSGE YRCQTNLSTL SDPVQLEVHI GWLLLQAPRW VFKEEDPIHL RCHSWKNTAL
130 140 150 160 170 180
HKVTYLQNGK GRKYFHHNSD FYIPKATLKD SGSYFCRGLF GSKNVSSETV NITITQGLAV
190 200 210 220 230 240
STISSFFPPG YQSTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS
250 260 270 280 290 300
HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA
310 320 330 340 350 360
LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP
370 380 390 400 410 420
ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
M.W.: Calculated molecular mass (kDa): 47.4; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI: 6.74
Calculated extinction coefficients (M-1 cm-1, at 280nm): 76945
Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Bioactivity: Binds human IgG or Fc-fusion proteins as measured by a functional ELISA with low affinity
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
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2. J Biol Chem. 270 (43): 25762 (1995)
3. J. Immunol. 157:1184 (1996)
4. Annu. Rev. Immunol.19:275 (2001).
5. Nature Rev. Immunol. 2:580 (2002)
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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