TACSTD2 (tumor-associated calcium signal transducer 2), also known as Trop2, M1S1, EGP1, GA733-1, gp50 and T16, is a single-pass type I transmembrane glycoprotein of the EPCAM family. It was originally defined by the monoclonal antibody GA733.  TACSTD2 may be formed by the retroposition of the EPCAM gene. TACSTD2 and EPCAM share ~49% amino acid identity and ~67% similarity.  TACSTD2 is highly expressed in a wide variety of epithelial cancers, while little or no expression in adult normal tissue. TACSTD2 is a cell surface receptor that transduces calcium signals and may play a role in tumor growth. TACSTD2 may also have cell adhesive functions. Mutations of the TACSTD2 gene are associated with gelatinous drop-like corneal dystrophy.  TACSTD2 is a target for immunotherapy treatment of human carcinomas.

TACSTD2 (tumor-associated calcium signal transducer 2), also known as Trop2, M1S1, EGP1, GA733-1, gp50 and T16, is a single-pass type I transmembrane glycoprotein of the EPCAM family. It was originally defined by the monoclonal antibody GA733.  TACSTD2 may be formed by the retroposition of the EPCAM gene. TACSTD2 and EPCAM share ~49% amino acid identity and ~67% similarity.  TACSTD2 is highly expressed in a wide variety of epithelial cancers, while little or no expression in adult normal tissue. TACSTD2 is a cell surface receptor that transduces calcium signals and may play a role in tumor growth. TACSTD2 may also have cell adhesive functions. Mutations of the TACSTD2 gene are associated with gelatinous drop-like corneal dystrophy.  TACSTD2 is a target for immunotherapy treatment of human carcinomas.

 

Product Details

 

Gene Symbol: TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1

 

NCBI Gene ID: 4070

 

Uniprot Entry: P09758

 

Construct Details: Expressed as a single-chain Fv (scFv) from anti-TACSTD2 VH and VL region fused with a scFv of anti-CD3e VH and VL region with a His-tag at the C-terminus. Exists as a monomer under non-reducing and reducing condition.

  

Source:  Mammalian cells stably expressing anti-TACSTD2:anti-CD3e  bispecific antibody (in a scFv-scFv’ format) and growing in chemical-defined media with no animal component or antibiotics

 

M.W.: Calculated molecular mass (kDa): 55.1; Estimated by SDS-PAGE under reducing condition(kDa): 55-60

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image inserted).

 

Formulation: Supplied at 0.25 mg/ml in sterile PBSpH7.4 (carrier and preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds TACSTD2/TROP2 extracellular domain (SKU#FCL2720) with high affinity and recognizes surface TACSTD2/TROP2 and CD3e in the context of TCR complex on T cells.  Potently promotes T cell-mediated anti-tumor cytotoxicity after engaging with tumor cell surface antigen TACSTD2/TROP2

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Proc. Natl. Acad. Sci. U.S.A. 86:27-31(1989)

2. Mol. Cell. Biol. 13:1507-1515(1993)

3. Int. J. Cancer 62:610-618(1995)

4. Nat. Genet. 21:420-423(1999)

5. Invest. Ophthalmol. Vis. Sci. 42:2762-2764(2001)

 

 

 

Additional supporting documents, including Product Datasheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.