IL31RA (interleukin-31 receptor subunit alpha) is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  IL31R consists of an extracellular region with 5 fibronectin type III (FN3) domains, a transmembrane domain, and a cytoplasmic tail. IL31RA functions either as a monomer, or as part of a receptor complex with OSMR (oncostatin M receptor). OSMR heterodimerizes with IL6ST (interleukin 6 signal transducer) or gp130 to form the type II OSMR complex and with IL31RA to form the IL31R complex, and thus transduces oncostatin M and IL31 induced signaling events.  IL31RA, with homology to IL6ST/gp130, is expressed on monocytes, and is involved in IL31 signaling via activation of STAT-3 and STAT-5.  Mutations in the IL31RA gene have been associated with familial primary localized cutaneous amyloidosis, 2 (PLCA2). 

IL31RA (interleukin-31 receptor subunit alpha) is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  IL31R consists of an extracellular region with 5 fibronectin type III (FN3) domains, a transmembrane domain, and a cytoplasmic tail. IL31RA functions either as a monomer, or as part of a receptor complex with OSMR (oncostatin M receptor). OSMR heterodimerizes with IL6ST (interleukin 6 signal transducer) or gp130 to form the type II OSMR complex and with IL31RA to form the IL31R complex, and thus transduces oncostatin M and IL31 induced signaling events.  IL31RA, with homology to IL6ST/gp130, is expressed on monocytes, and is involved in IL31 signaling via activation of STAT-3 and STAT-5.  Mutations in the IL31RA gene have been associated with familial primary localized cutaneous amyloidosis, 2 (PLCA2). 

 

Product Details

 

Gene Symbol: IL31RA; CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; IL-31Ralpha; ZcytoR17; PRO21384

 

NCBI Gene ID: 133396

 

Uniprot Entry: Q8NI17

 

Construct Details: Full length human IL31RA gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2199 (bp)

 

Gene Insert Sequence:  

ATGATGTGGACCTGGGCACTGTGGATGCTCCCCTCACTCTGCAAATTCAGCCTGGCAGCTCTGCCAGCTAAGCCTGAGAA

CATTTCCTGTGTCTACTACTATAGGAAAAATTTAACCTGCACTTGGAGTCCAGGAAAGGAAACCAGTTATACCCAGTACA

CAGTTAAGAGAACTTACGCTTTTGGAGAAAAACATGATAATTGTACAACCAATAGTTCTACAAGTGAAAATCGTGCTTCG

TGCTCTTTTTTCCTTCCAAGAATAACGATCCCAGATAATTATACCATTGAGGTGGAAGCTGAAAATGGAGATGGTGTAAT

TAAATCTCATATGACATACTGGAGATTAGAGAACATAGCGAAAACTGAACCACCTAAGATTTTCCGTGTGAAACCAGTTT

TGGGCATCAAACGAATGATTCAAATTGAATGGATAAAGCCTGAGTTGGCGCCTGTTTCATCTGATTTAAAATACACACTT

CGATTCAGGACAGTCAACAGTACCAGCTGGATGGAAGTCAACTTCGCTAAGAACCGTAAGGATAAAAACCAAACGTACAA

CCTCACGGGGCTGCAGCCTTTTACAGAATATGTCATAGCTCTGCGATGTGCGGTCAAGGAGTCAAAGTTCTGGAGTGACT

GGAGCCAAGAAAAAATGGGAATGACTGAGGAAGAAGCTCCATGTGGCCTGGAACTGTGGAGAGTCCTGAAACCAGCTGAG

GCGGATGGAAGAAGGCCAGTGCGGTTGTTATGGAAGAAGGCAAGAGGAGCCCCAGTCCTAGAGAAAACACTTGGCTACAA

CATATGGTACTATCCAGAAAGCAACACTAACCTCACAGAAACAATGAACACTACTAACCAGCAGCTTGAACTGCATCTGG

GAGGCGAGAGCTTTTGGGTGTCTATGATTTCTTATAATTCTCTTGGGAAGTCTCCAGTGGCCACCCTGAGGATTCCAGCT

ATTCAAGAAAAATCATTTCAGTGCATTGAGGTCATGCAGGCCTGCGTTGCTGAGGACCAGCTAGTGGTGAAGTGGCAAAG

CTCTGCTCTAGACGTGAACACTTGGATGATTGAATGGTTTCCGGATGTGGACTCAGAGCCCACCACCCTTTCCTGGGAAT

CTGTGTCTCAGGCCACGAACTGGACGATCCAGCAAGATAAATTAAAACCTTTCTGGTGCTATAACATCTCTGTGTATCCA

ATGTTGCATGACAAAGTTGGCGAGCCATATTCCATCCAGGCTTATGCCAAAGAAGGCGTTCCATCAGAAGGTCCTGAGAC

CAAGGTGGAGAACATTGGCGTGAAGACGGTCACGATCACATGGAAAGAGATTCCCAAGAGTGAGAGAAAGGGTATCATCT

GCAACTACACCATCTTTTACCAAGCTGAAGGTGGAAAAGGATTCTCCAAGACAGTCAATTCCAGCATCTTGCAGTACGGC

CTGGAGTCCCTGAAACGAAAGACCTCTTACATTGTTCAGGTCATGGCCAGCACCAGTGCTGGGGGAACCAACGGGACCAG

CATAAATTTCAAGACATTGTCATTCAGTGTCTTTGAGATTATCCTCATAACTTCTCTGATTGGTGGAGGCCTTCTTATTC

TCATTATCCTGACAGTGGCATATGGTCTCAAAAAACCCAACAAATTGACTCATCTGTGTTGGCCCACCGTTCCCAACCCT

GCTGAAAGTAGTATAGCCACATGGCATGGAGATGATTTCAAGGATAAGCTAAACCTGAAGGAGTCTGATGACTCTGTGAA

CACAGAAGACAGGATCTTAAAACCATGTTCCACCCCCAGTGACAAGTTGGTGATTGACAAGTTGGTGGTGAACTTTGGGA

ATGTTCTGCAAGAAATTTTCACAGATGAAGCCAGAACGGGTCAGGAAAACAATTTAGGAGGGGAAAAGAATGGGTATGTG

ACCTGCCCCTTCAGGCCTGATTGTCCCCTGGGGAAAAGTTTTGAGGAGCTCCCAGTTTCACCTGAGATTCCGCCCAGAAA

ATCCCAATACCTACGTTCGAGGATGCCAGAGGGGACCCGCCCAGAAGCCAAAGAGCAGCTTCTCTTTTCTGGTCAAAGTT

TAGTACCAGATCATCTGTGTGAGGAAGGAGCCCCAAATCCATATTTGAAAAATTCAGTGACAGCCAGGGAATTTCTTGTG

TCTGAAAAACTTCCAGAGCACACCAAGGGAGAAGTCTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.