CD360, also known as IL21R (Interleukin-21 receptor), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily.  CD360/IL21R consists of an extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation. CD360/IL21R acts as a receptor for IL21 and forms a heterodimeric receptor complex with the common gamma-chain, interleukin 2 receptor gamma (IL2RG), a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. CD360/IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.  IL21 binding leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies suggest a role for the IL21R/CD360 gene in regulating immunoglobulin production. The mutations affecting the IL21R/CD360 gene is associated with IL21R immunodeficiency (IL21RID), an autosomal recessive primary immunodeficiency characterized by B- and T-cell defects and variable dysfunction of NK cells. Patients have normal numbers of lymphocytes, but show defective class-switched B-cells, low IgG, defective antibody response, and defective T-cell responses to certain antigens.

CD360, also known as IL21R (Interleukin-21 receptor), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily.  CD360/IL21R consists of an extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation. CD360/IL21R acts as a receptor for IL21 and forms a heterodimeric receptor complex with the common gamma-chain, interleukin 2 receptor gamma (IL2RG), a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. CD360/IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.  IL21 binding leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies suggest a role for the IL21R/CD360 gene in regulating immunoglobulin production. The mutations affecting the IL21R/CD360 gene is associated with IL21R immunodeficiency (IL21RID), an autosomal recessive primary immunodeficiency characterized by B- and T-cell defects and variable dysfunction of NK cells. Patients have normal numbers of lymphocytes, but show defective class-switched B-cells, low IgG, defective antibody response, and defective T-cell responses to certain antigens.

 

Product Details

 

Gene Symbol: IL21R; CD360; NILR; IL21-R

 

NCBI Gene ID: 50615

 

Uniprot Entry: Q9HBE5

 

Construct Details: Full length human IL21R/CD360 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1617 (bp)

 

Gene Insert Sequence:  

ATGCCGCGTGGCTGGGCCGCCCCCTTGCTCCTGCTGCTGCTCCAGGGAGGCTGGGGCTGCCCCGACCTCG

TCTGCTACACCGATTACCTCCAGACGGTCATCTGCATCCTGGAAATGTGGAACCTCCACCCCAGCACGCT

CACCCTTACCTGGCAAGACCAGTATGAAGAGCTGAAGGACGAGGCCACCTCCTGCAGCCTCCACAGGTCG

GCCCACAATGCCACGCATGCCACCTACACCTGCCACATGGATGTATTCCACTTCATGGCCGACGACATTT

TCAGTGTCAACATCACAGACCAGTCTGGCAACTACTCCCAGGAGTGTGGCAGCTTTCTCCTGGCTGAGAG

CATCAAGCCGGCTCCCCCTTTCAACGTGACTGTGACCTTCTCAGGACAGTATAATATCTCCTGGCGCTCA

GATTACGAAGACCCTGCCTTCTACATGCTGAAGGGCAAGCTTCAGTATGAGCTGCAGTACAGGAACCGGG

GAGACCCCTGGGCTGTGAGTCCGAGGAGAAAGCTGATCTCAGTGGACTCAAGAAGTGTCTCCCTCCTCCC

CCTGGAGTTCCGCAAAGACTCGAGCTATGAGCTGCAGGTGCGGGCAGGGCCCATGCCTGGCTCCTCCTAC

CAGGGGACCTGGAGTGAATGGAGTGACCCGGTCATCTTTCAGACCCAGTCAGAGGAGTTAAAGGAAGGCT

GGAACCCTCACCTGCTGCTTCTCCTCCTGCTTGTCATAGTCTTCATTCCTGCCTTCTGGAGCCTGAAGAC

CCATCCATTGTGGAGGCTATGGAAGAAGATATGGGCCGTCCCCAGCCCTGAGCGGTTCTTCATGCCCCTG

TACAAGGGCTGCAGCGGAGACTTCAAGAAATGGGTGGGTGCACCCTTCACTGGCTCCAGCCTGGAGCTGG

GACCCTGGAGCCCAGAGGTGCCCTCCACCCTGGAGGTGTACAGCTGCCACCCACCACGGAGCCCGGCCAA

GAGGCTGCAGCTCACGGAGCTACAAGAACCAGCAGAGCTGGTGGAGTCTGACGGTGTGCCCAAGCCCAGC

TTCTGGCCGACAGCCCAGAACTCGGGGGGCTCAGCTTACAGTGAGGAGAGGGATCGGCCATACGGCCTGG

TGTCCATTGACACAGTGACTGTGCTAGATGCAGAGGGGCCATGCACCTGGCCCTGCAGCTGTGAGGATGA

CGGCTACCCAGCCCTGGACCTGGATGCTGGCCTGGAGCCCAGCCCAGGCCTAGAGGACCCACTCTTGGAT

GCAGGGACCACAGTCCTGTCCTGTGGCTGTGTCTCAGCTGGCAGCCCTGGGCTAGGAGGGCCCCTGGGAA

GCCTCCTGGACAGACTAAAGCCACCCCTTGCAGATGGGGAGGACTGGGCTGGGGGACTGCCCTGGGGTGG

CCGGTCACCTGGAGGGGTCTCAGAGAGTGAGGCGGGCTCACCCCTGGCCGGCCTGGATATGGACACGTTT

GACAGTGGCTTTGTGGGCTCTGACTGCAGCAGCCCTGTGGAGTGTGACTTCACCAGCCCCGGGGACGAAG

GACCCCCCCGGAGCTACCTCCGCCAGTGGGTGGTCATTCCTCCGCCACTTTCGAGCCCTGGACCCCAGGC

CAGCTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.