CD129, also known as IL9R (Interleukin-9 receptor), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily.  CD129 consists of an extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation. CD129/IL9R acts as a receptor for both IL9. The functional IL9 receptor complex requires CD129 as well as the interleukin 2 receptor, gamma (IL2RG), a common gamma subunit shared by the receptors of many different cytokines. IL9 binding leads to the activation of various JAK kinases and STAT proteins, which connect to different downstream biological responses. The CD129/IL9R gene is located at the pseudoautosomal regions of X and Y chromosomes. Genetic studies suggested an association of the CD129/IL9R gene with the development of asthma. 

CD129, also known as IL9R (Interleukin-9 receptor), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily.  CD129 consists of an extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation. CD129/IL9R acts as a receptor for both IL9. The functional IL9 receptor complex requires CD129 as well as the interleukin 2 receptor, gamma (IL2RG), a common gamma subunit shared by the receptors of many different cytokines. IL9 binding leads to the activation of various JAK kinases and STAT proteins, which connect to different downstream biological responses. The CD129/IL9R gene is located at the pseudoautosomal regions of X and Y chromosomes. Genetic studies suggested an association of the CD129/IL9R gene with the development of asthma. 

 

Product Details

 

Gene Symbol: IL9R; CD129; IL-9R

 

NCBI Gene ID: 3581

 

Uniprot Entry: P24394

 

Construct Details: Full length human IL9R/CD129 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1566 (bp)

 

Gene Insert Sequence:  

ATGGGACTGGGCAGATGCATCTGGGAAGGCTGGACCTTGGAGAGTGAGGCCCTGAGGCGAGACATGGGCA

CCTGGCTCCTGGCCTGCATCTGCATCTGCACCTGTGTCTGCTTGGGAGTCTCTGTCACAGGGGAAGGACA

AGGGCCAAGGTCTAGAACCTTCACCTGCCTCACCAACAACATTCTCAGGATCGATTGCCACTGGTCTGCC

CCAGAGCTGGGACAGGGCTCCAGCCCCTGGCTCCTCTTCACCAGCAACCAGGCTCCTGGCGGCACACATA

AGTGCATCTTGCGGGGCAGTGAGTGCACCGTCGTGCTGCCACCTGAGGCAGTGCTCGTGCCATCTGACAA

TTTCACCATCACTTTCCACCACTGCATGTCTGGGAGGGAGCAGGTCAGCCTGGTGGACCCGGAGTACCTG

CCCCGGAGACACGTTAAGCTGGACCCGCCCTCTGACTTGCAGAGCAACATCAGTTCTGGCCACTGCATCC

TGACCTGGAGCATCAGTCCTGCCTTGGAGCCAATGACCACACTTCTCAGCTATGAGCTGGCCTTCAAGAA

GCAGGAAGAGGCCTGGGAGCAGGCCCAGCACAGGGATCACATTGTCGGGGTGACCTGGCTTATACTTGAA

GCCTTTGAGCTGGACCCTGGCTTTATCCATGAGGCCAGGCTGCGTGTCCAGATGGCCACACTGGAGGATG

ATGTGGTAGAGGAGGAGCGTTATACAGGCCAGTGGAGTGAGTGGAGCCAGCCTGTGTGCTTCCAGGCTCC

CCAGAGACAAGGCCCTCTGATCCCACCCTGGGGGTGGCCAGGCAACACCCTTGTTGCTGTGTCCATCTTT

CTCCTGCTGACTGGCCCGACCTACCTCCTGTTCAAGCTGTCGCCCAGGGTGAAGAGAATCTTCTACCAGA

ACGTGCCCTCTCCAGCGATGTTCTTCCAGCCCCTCTACAGTGTACACAATGGGAACTTCCAGACTTGGAT

GGGGGCCCACGGGGCCGGTGTGCTGTTGAGCCAGGACTGTGCTGGCACCCCACAGGGAGCCTTGGAGCCC

TGCGTCCAGGAGGCCACTGCACTGCTCACTTGTGGCCCAGCGCGTCCTTGGAAATCTGTGGCCCTGGAGG

AGGAACAGGAGGGCCCTGGGACCAGGCTCCCGGGGAACCTGAGCTCAGAGGATGTGCTGCCAGCAGGGTG

TACGGAGTGGAGGGTACAGACGCTTGCCTATCTGCCACAGGAGGACTGGGCCCCCACGTCCCTGACTAGG

CCGGCTCCCCCAGACTCAGAGGGCAGCAGGAGCAGCAGCAGCAGCAGCAGCAGCAACAACAACAACTACT

GTGCCTTGGGCTGCTATGGGGGATGGCACCTCTCAGCCCTCCCAGGAAACACACAGAGCTCTGGGCCCAT

CCCAGCCCTGGCCTGTGGCCTTTCTTGTGACCATCAGGGCCTGGAGACCCAGCAAGGAGTTGCCTGGGTG

CTGGCTGGTCACTGCCAGAGGCCTGGGCTGCATGAGGACCTCCAGGGCATGTTGCTCCCTTCTGTCCTCA

GCAAGGCTCGGTCCTGGACATTCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.