ULBP1 (UL16 binding protein 1) is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. ULBP1 is a GPI-anchored glycoprotein that belongs to the MHC-I family of Immunoglobulin superfamily (IgSF).  Binding of the ligand including ULBP1, ULBP2 and ULBP3, to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt, resulting in the production of cytokines and chemokines. ULBP1 does not bind to beta2-microglobulin. In cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein. The interaction with UL16 blocked the interaction with the KLRK1/NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.

ULBP1 (UL16 binding protein 1) is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. ULBP1 is a GPI-anchored glycoprotein that belongs to the MHC-I family of Immunoglobulin superfamily (IgSF).  Binding of the ligand including ULBP1, ULBP2 and ULBP3, to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt, resulting in the production of cytokines and chemokines. ULBP1 does not bind to beta2-microglobulin. In cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein. The interaction with UL16 blocked the interaction with the KLRK1/NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.

 

Product Details

 

Gene Symbol: ULBP1; N2DL1; N2DL-1; RAET1I; NKG2DL1

 

NCBI Gene ID: 80329

 

Uniprot Entry: Q9BZM6

 

Construct Details: Full length human ULBP1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and with or without a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 735 (bp)

 

Gene Insert Sequence:  

ATGGCAGCGGCCGCCAGCCCCGCGTTCCTTCTGTGCCTCCCGCTTCTGCACCTGCTGTCTGGCTGGTCCC

GGGCAGGATGGGTCGACACACACTGTCTTTGCTATGACTTCATCATCACTCCTAAGTCCAGACCTGAACC

ACAGTGGTGTGAAGTTCAAGGCCTGGTGGATGAAAGGCCTTTTCTTCACTATGACTGTGTTAACCACAAG

GCCAAAGCCTTTGCTTCTCTGGGGAAGAAAGTCAATGTCACAAAAACCTGGGAAGAACAAACTGAAACAC

TAAGAGACGTGGTGGATTTCCTTAAAGGGCAACTGCTTGACATTCAAGTGGAGAATTTAATACCCATTGA

GCCCCTCACCCTGCAGGCCAGGATGTCTTGTGAGCATGAAGCCCATGGACACGGCAGAGGATCTTGGCAG

TTCCTCTTCAATGGACAGAAGTTCCTCCTCTTTGACTCAAACAACAGAAAGTGGACAGCACTTCATCCTG

GAGCCAAGAAGATGACAGAGAAGTGGGAGAAGAACAGGGATGTGACCATGTTCTTCCAGAAGATTTCACT

GGGGGATTGTAAGATGTGGCTTGAAGAATTTTTGATGTACTGGGAACAAATGCTGGATCCAACAAAACCA

CCCTCTCTGGCCCCAGGCACAACCCAACCCAAGGCCATGGCCACCACCCTCAGTCCCTGGAGCCTTCTCA

TCATCTTCCTCTGCTTCATTCTAGCTGGCAGATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.