CD40L (CD40 ligand), also known as TNFSF5 and CD154, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily (TNFSF). CD40L/CD154 is a single-pass type II transmebrane glycoprotein consisting of a 22 amino acid (aa) cytoplasmic domain, a 24-aa transmembrane domain (TM) and a 215 aa extracellular domain (ECD). CD40L is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. CD40L co-stimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. A defect in the CD40L gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

CD40L (CD40 ligand), also known as TNFSF5 and CD154, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily (TNFSF). CD40L/CD154 is a single-pass type II transmebrane glycoprotein consisting of a 22 amino acid (aa) cytoplasmic domain, a 24-aa transmembrane domain (TM) and a 215 aa extracellular domain (ECD). CD40L is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. CD40L co-stimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. A defect in the CD40L gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

  

Product Details

 

Gene Symbol: CD40L; CD154; TNFSF5; IGM; IMD3; TRAP; gp39;  HIGM1; T-BAM; hCD40L

  

NCBI Gene ID: 959

 

Uniprot Entry: P29965

 

Construct Details: The recombinant human CD40L-Fc fusion is expressed as a 443 amino acid protein consisting of His47 - Leu261 region of CD40L and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human CD40L-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

HRRLDKIEDE RNLHEDFVFM KTIQRCNTGE RSLSLLNCEE IKSQFEGFVK DIMLNKEETK 

        70         80         90        100        110        120 

KENSFEMQKG DQNPQIAAHV ISEASSKTTS VLQWAEKGYY TMSNNLVTLE NGKQLTVKRQ 

       130        140        150        160        170        180 

GLYYIYAQVT FCSNREASSQ APFIASLCLK SPGRFERILL RAANTHSSAK PCGQQSIHLG 

       190        200        210        220        230        240 

GVFELQPGAS VFVNVTDPSQ VSHGTGFTSF GLLKLSTGTH TCPPCPAPEL LGGPSVFLFP 

       250        260        270        280        290        300 

PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS 

       310        320        330        340        350        360 

VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS 

       370        380        390        400        410        420 

LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS 

       430        440 

CSVMHEALHN HYTQKSLSLS PGK 

 

M.W.: Calculated molecular mass (kDa): 49.8; Estimated by SDS-PAGE under reducing condition (kDa): 65-70 

 

Calculated PI: 7.97

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 48985

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity:  Binds to its receptor CD40 and induces CD40-mediated signaling activities. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.