CD14 is a GPI-anchored surface glycoprotein of the LRR (leucine-rich repeat) containing protein family.  CD14 contains 11 LRR domains in the extracellular region. CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide (LPS). The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS. Thus CD14 belongs to the LPS receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial LPS. CD14 aslo acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface. CD14 also binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL.

CD14 is a GPI-anchored surface glycoprotein of the LRR (leucine-rich repeat) containing protein family.  CD14 contains 11 LRR domains in the extracellular region. CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide (LPS). The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS. Thus CD14 belongs to the LPS receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial LPS. CD14 aslo acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface. CD14 also binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL.

 

Product Details

 

Gene Symbol: CD14

 

NCBI Gene ID: 929

 

Uniprot Entry: P08571

 

Construct Details: The recombinant human CD14-Fc fusion is expressed as a 553 amino acid protein consisting of Thr20 - Met345 region of CD14 and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human CD14-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP 

        70         80         90        100        110        120 

RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA 

       130        140        150        160        170        180 

TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL 

       190        200        210        220        230        240 

DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN  

       250        260        270        280        290        300 

SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP 

       310        320        330        340        350        360 

EVDNLTLDGN PFLVPGTALP HEGSMVDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS 

       370        380        390        400        410        420 

RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL 

       430        440        450        460        470        480 

NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP 

       490        500        510        520        530        540 

SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN 

       550 

HYTQKSLSLS PGK 

 

M.W.: Calculated molecular mass (kDa): 60.7; Estimated by SDS-PAGE under reducing condition (kDa): 65-70 

 

Calculated PI: 6.04

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 66765

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity:  Binds LPS and electronegative LDL (LDL-) and inhibits the cytokine release induced by LDL

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including product data sheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.