CD132, also known as IL2RG (interleukin 2 receptor subunit gamma), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  CD132 consists of an extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD132 is a common component of the receptor complexeas for a variety of interleukins, including IL2, IL4, IL7, IL15, IL21 and probably IL13.  CD132 is thus referred to as the common gamma chain. Mutations in the CD132 gene cause severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

CD132, also known as IL2RG (interleukin 2 receptor subunit gamma), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  CD132 consists of an extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD132 is a common component of the receptor complexeas for a variety of interleukins, including IL2, IL4, IL7, IL15, IL21 and probably IL13.  CD132 is thus referred to as the common gamma chain. Mutations in the CD132 gene cause severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

 

Product Details

 

Gene Symbol: IL2RG; CD132; P64; CIDX; IMD4; SCIDX; IL-2RG; SCIDX1; XCID; XSCID

 

NCBI Gene ID: 3561

 

Uniprot Entry: P31785

 

Construct Details: The recombinant human CD132/IL2RG-Fc fusion is expressed as a 468 amino acid protein consisting of Leu23 - Ala262 region of CD132/IL2RG and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human CD132/IL2RG-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

LNTTILTPNG NEDTTADFFL TTMPTDSLSV STLPLPEVQC FVFNVEYMNC TWNSSSEPQP  

        70         80         90        100        110        120 

TNLTLHYWYK NSDNDKVQKC SHYLFSEEIT SGCQLQKKEI HLYQTFVVQL QDPREPRRQA 

       130        140        150        160        170        180 

TQMLKLQNLV IPWAPENLTL HKLSESQLEL NWNNRFLNHC LEHLVQYRTD WDHSWTEQSV 

       190        200        210        220        230        240 

DYRHKFSLPS VDGQKRYTFR VRSRFNPLCG SAQHWSEWSH PIHWGSNTSK ENPFLFALEA 

       250        260        270        280        290        300 

STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV 

       310        320        330        340        350        360 

DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA 

       370        380        390        400        410        420 

KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD 

       430        440        450        460 

SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 53.8; Estimated by SDS-PAGE under reducing condition (kDa): 75-80 

 

Calculated PI: 6.33

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 97580

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Science 257:379-382(1992) 

2. Science 262:1874, 1880, 1877 (1993)

3. J. Biol. Chem. 268:13601-13608(1993)

4. Hum. Mol. Genet. 2:1099-1104(1993)

5. N. Engl. J. Med. 335:1563-1567(1996)

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.