LILRB2 (Leukocyte immunoglobulin like receptor B2), also known as CD85d, LIR2 (Leukocyte immunoglobulin-like receptor 2), ILT4 (Ig-like transcript 4), and MIR10 (myeloid inhibitory receptor 10), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB2 has 4 Ig-like C2 type domains and 3 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses.  LILRB2 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected at low levelson natural killer (NK) cells.  Like LILRB1, LILRB2 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB2 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB2 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB2 also binds FCGR1A.  

LILRB2 (Leukocyte immunoglobulin like receptor B2), also known as CD85d, LIR2 (Leukocyte immunoglobulin-like receptor 2), ILT4 (Ig-like transcript 4), and MIR10 (myeloid inhibitory receptor 10), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB2 has 4 Ig-like C2 type domains and 3 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses.  LILRB2 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected at low levelson natural killer (NK) cells.  Like LILRB1, LILRB2 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB2 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB2 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB2 also binds FCGR1A.  

 

Product Details

 

Gene Symbol: LILRB2; ILT4; LIR2; MIR10; CD85d; ILT-4; LIR-2; MIR-10

 

NCBI Gene ID: 

 

Uniprot Entry: Q8N423

 

Construct Details: Full length human LILRB2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and with or without a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1797 (bp)

 

Gene Insert Sequence:  

ATGACCCCCATCGTCACAGTCCTGATCTGTCTCGGGCTGAGTCTGGGCCCCAGGACCCGCGTGCAGACAG

GGACCATCCCCAAGCCCACCCTGTGGGCTGAGCCAGACTCTGTGATCACCCAGGGGAGTCCCGTCACCCT

CAGTTGTCAGGGGAGCCTTGAAGCCCAGGAGTACCGTCTATATAGGGAGAAAAAATCAGCATCTTGGATT

ACACGGATACGACCAGAGCTTGTGAAGAACGGCCAGTTCCACATCCCATCCATCACCTGGGAACACACAG

GGCGATATGGCTGTCAGTATTACAGCCGCGCTCGGTGGTCTGAGCTCAGTGACCCCCTGGTGCTGGTGAT

GACAGGAGCCTACCCAAAACCCACCCTCTCAGCCCAGCCCAGCCCTGTGGTGACCTCAGGAGGAAGGGTG

ACCCTCCAGTGTGAGTCACAGGTGGCATTTGGCGGCTTCATTCTGTGTAAGGAAGGAGAAGATGAACACC

CACAATGCCTGAACTCCCAGCCCCATGCCCGTGGGTCGTCCCGCGCCATCTTCTCCGTGGGCCCCGTGAG

CCCGAATCGCAGGTGGTCGCACAGGTGCTATGGTTATGACTTGAACTCTCCCTATGTGTGGTCTTCACCC

AGTGATCTCCTGGAGCTCCTGGTCCCAGGTGTTTCTAAGAAGCCATCACTCTCAGTGCAGCCGGGTCCTG

TCATGGCCCCTGGGGAAAGCCTGACCCTCCAGTGTGTCTCTGATGTCGGCTATGACAGATTTGTTCTGTA

CAAGGAGGGGGAACGTGACCTTCGCCAGCTCCCTGGCCGGCAGCCCCAGGCTGGGCTCTCCCAGGCCAAC

TTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTACGGTGCACACAACCTCTCCT

CTGAGTGCTCGGCCCCCAGCGACCCCCTGGACATCCTGATCACAGGACAGATCCGTGGCACACCCTTCAT

CTCAGTGCAGCCAGGCCCCACAGTGGCCTCAGGAGAGAACGTGACCCTGCTGTGTCAGTCATGGCGGCAG

TTCCACACTTTCCTTCTGACCAAGGCGGGAGCAGCTGATGCCCCACTCCGTCTAAGATCAATACACGAAT

ATCCTAAGTACCAGGCTGAATTCCCCATGAGTCCTGTGACCTCAGCCCACGCGGGGACCTACAGGTGCTA

CGGCTCACTCAACTCCGACCCCTACCTGCTGTCTCACCCCAGTGAGCCCCTGGAGCTCGTGGTCTCAGGA

CCCTCCATGGGTTCCAGCCCCCCACCCACCGGTCCCATCTCCACACCTGCAGGCCCTGAGGACCAGCCCC

TCACCCCCACTGGGTCGGATCCCCAAAGTGGTCTGGGAAGGCACCTGGGGGTTGTGATCGGCATCTTGGT

GGCCGTCGTCCTACTGCTCCTCCTCCTCCTCCTCCTCTTCCTCATCCTCCGACATCGACGTCAGGGCAAA

CACTGGACATCGACCCAGAGAAAGGCTGATTTCCAACATCCTGCAGGGGCTGTGGGGCCAGAGCCCACAG

ACAGAGGCCTGCAGTGGAGGTCCAGCCCAGCTGCCGACGCCCAGGAAGAAAACCTCTATGCTGCCGTGAA

GGACACACAGCCTGAAGATGGGGTGGAGATGGACACTCGGGCTGCTGCATCTGAAGCCCCCCAGGATGTG

ACCTACGCCCAGCTGCACAGCTTGACCCTCAGACGGAAGGCAACTGAGCCTCCTCCATCCCAGGAAAGGG

AACCTCCAGCTGAGCCCAGCATCTACGCCACCCTGGCCATCCACTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. J. Exp. Med. 186:1809-1818(1997)

2. J. Immunol. 159:5192-5196(1997)

3. Eur. J. Immunol. 28:3423-3434(1998)

4. Immunity 11:603-613(1999) 

5. J. Immunol. 168:3351-3359(2002)

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.