IL17 (interleukin 17) is a proinflammatory cytokine secreted by activated T-lymphocytes IL17 induces the maturation of hematopoietic precursors into neutrophils.  IL-17Ra (IL17 receptor A), also known as, IL17R and CD217, is a single pass type I transmembrane glycoprotein that acts as a low affinity receptor for IL17A, C and F.  IL17RA binds IL17A or IL17F homodimers as part of a heterodimeric complex with IL17RC.  IL17RA also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC.  IL17RA acts as a receptor for IL17C as part of a heterodimeric complex with IL17RE. Activation of IL17RA-mediated signaling pathway leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. IL17 and its receptor IL17RA play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis (RA). Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5), which is a primary immunodeficiency disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. 

IL17 (interleukin 17) is a proinflammatory cytokine secreted by activated T-lymphocytes IL17 induces the maturation of hematopoietic precursors into neutrophils.  IL-17Ra (IL17 receptor A), also known as, IL17R and CD217, is a single pass type I transmembrane glycoprotein that acts as a low affinity receptor for IL17A, C and F.  IL17RA binds IL17A or IL17F homodimers as part of a heterodimeric complex with IL17RC.  IL17RA also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC.  IL17RA acts as a receptor for IL17C as part of a heterodimeric complex with IL17RE. Activation of IL17RA-mediated signaling pathway leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. IL17 and its receptor IL17RA play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis (RA). Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5), which is a primary immunodeficiency disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. 

 

Product Details

 

Gene Symbol: IL17RA; CD217; IL17R; CANDF5; CDw217; IL-17RA; hIL-17R

 

NCBI Gene ID: 23765

 

Uniprot Entry: Q96F46

 

Construct Details: The recombinant human IL17Ra-Fc fusion is expressed as a 524 amino acid protein consisting of Leu33 - Met318 region of IL17Ra and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human IL17Ra-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

LRLLDHRALV CSQPGLNCTV KNSTCLDDSW IHPRNLTPSS PKDLQIQLHF AHTQQGDLFP 

        70         80         90        100        110        120 

VAHIEWTLQT DASILYLEGA ELSVLQLNTN ERLCVRFEFL SKLRHHHRRW RFTFSHFVVD 

       130        140        150        160        170        180 

PDQEYEVTVH HLPKPIPDGD PNHQSKNFLV PDCEHARMKV TTPCMSSGSL WDPNITVETL 

       190        200        210        220        230        240 

EAHQLRVSFT LWNESTHYQI LLTSFPHMEN HSCFEHMHHI PAPRPEEFHQ RSNVTLTLRN 

       250        260        270        280        290        300 

LKGCCRHQVQ IQPFFSSCLN DCLRHSATVS CPEMPDTPEP IPDYMPSTTE NLYFQGSTGT 

       310        320        330        340        350        360 

HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE 

       370        380        390        400        410        420 

VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP 

       430        440        450        460        470        480 

REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS 

       490        500        510        520 

FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK 

 

M.W.: Calculated molecular mass (kDa): 60; Estimated by SDS-PAGE under reducing condition (kDa): 90-95

 

Calculated PI: 6.31

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 71485

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity:  Binds to human IL17A and IL17F proteins by ELISA and inhibits IL-17-induced IL-6 secretion in fibroblast cells

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cytokine 9:794-800(1997) 

2. J. Immunol. 177:36-39(2006)

3. J. Immunol. 179:5462-5473(2007)

4. J. Immunol. 181:2799-2805(2008) 

5. Nat. Immunol. 10:1245-1251(2009) 

6. Science 332:65-68(2011) 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.