OX40L (OX40 ligand), also known as TNFSF4, gp34 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. OX40L is a single-pass type II transmebrane glycoprotein consisting of a 23 amino acid (aa) cytoplasmic domain, a 27-aa transmembrane domain (TM) and a 133 aa extracellular domain (ECD). OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40L exists as a homotrimer. OX40L binds to OX40/CD134, a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the regulation of T-cell-mediated immunity that includes B7, CD40L, CD30L, CD27L/CD70 and 4-1BBL. OX40L functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in the OX40L gene have been associated with Sjogren's syndrome and systemic lupus erythematosus.  The interaction of OX40L:OX40 is also involved in the pathogenesis of carotid artery disease and cancer.  Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

OX40L (OX40 ligand), also known as TNFSF4, gp34 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. OX40L is a single-pass type II transmebrane glycoprotein consisting of a 23 amino acid (aa) cytoplasmic domain, a 27-aa transmembrane domain (TM) and a 133 aa extracellular domain (ECD). OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40L exists as a homotrimer. OX40L binds to OX40/CD134, a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the regulation of T-cell-mediated immunity that includes B7, CD40L, CD30L, CD27L/CD70 and 4-1BBL. OX40L functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in the OX40L gene have been associated with Sjogren's syndrome and systemic lupus erythematosus.  The interaction of OX40L:OX40 is also involved in the pathogenesis of carotid artery disease and cancer.  Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

  

Product Details

 

Gene Symbol: LIGHT; TNFSF14; LTg; CD258; HVEML

  

NCBI Gene ID: 7292

 

Uniprot Entry: P23510

 

Construct Details: The recombinant human OX40L-Fc fusion is expressed as a 361 amino acid protein consisting of Gln51 - Leu183 region of OX40L and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing condition.  

 

Source: Human cells stably expressing human OX40L-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

 

        10         20         30         40         50         60 

QVSHRYPRIQ SIKVQFTEYK KEKGFILTSQ KEDEIMKVQN NSVIINCDGF YLISLKGYFS 

        70         80         90        100        110        120 

QEVNISLHYQ KDEEPLFQLK KVRSVNSLMV ASLTYKDKVY LNVTTDNTSL DDFHVNGGEL 

       130        140        150        160        170        180 

ILIHQNPGEF CVLSTGTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV 

       190        200        210        220        230        240 

SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK 

       250        260        270        280        290        300 

ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ  

       310        320        330        340        350        360 

PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG 

 

K 

  

M.W.: Calculated molecular mass (kDa): 41; Estimated by SDS-PAGE under reducing condition (kDa): 50-55

 

Calculated PI: 6.9

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 46340

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its receptor OX40 protein and induces OX40-mediated signaling activities

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Mol. Cell. Biol. 11:1313-1325(1991)

2. J. Exp. Med. 180:757-762(1994)

3. EMBO J. 13:3992-4001(1994)

4. Nat. Genet. 40:83-89(2008)

5. Structure 14:1321-1330(2006)

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.