LIGHT, also known as TNFSF14, HVEML and CD258, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. LIGHT is a single-pass type II transmebrane glycoprotein consisting of a 37 amino acid (aa) cytoplasmic domain, a 21-aa transmembrane domain (TM) and a 182 aa extracellular domain (ECD). LIGHT is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. LIGHT is known to bind at lest 2 TNF receptor superfamily (TNFRSF) members: HVEM, which is predominantly expressed by T cells, and LTβR (lymphotoxin β receptor), which is expressed by stromal cells and nonlymphoid hematopoietic cells. LIGHT also binds to a soluble TNFRSF member, DcR3 (decoy receptor 3), which can modulate the function of LIGHT. LIGHT functions as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. LIGHT activates NF-kappaB, stimulates the proliferation of T cells, and triggers apoptosis of various tumor cells. LIGHT is also reported to prevent TNF-mediated apoptosis in primary hepatocyte. LIGHT has been shown to suppress tumor formation through the up-regulation of intercellular adhesion molecule 1 and the increase of lymphocyte adhesion to cancer cells. LIGHT is thus a promising target for cancer treatment.

LIGHT, also known as TNFSF14, HVEML and CD258, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. LIGHT is a single-pass type II transmebrane glycoprotein consisting of a 37 amino acid (aa) cytoplasmic domain, a 21-aa transmembrane domain (TM) and a 182 aa extracellular domain (ECD). LIGHT is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. LIGHT is known to bind at lest 2 TNF receptor superfamily (TNFRSF) members: HVEM, which is predominantly expressed by T cells, and LTβR (lymphotoxin β receptor), which is expressed by stromal cells and nonlymphoid hematopoietic cells. LIGHT also binds to a soluble TNFRSF member, DcR3 (decoy receptor 3), which can modulate the function of LIGHT. LIGHT functions as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. LIGHT activates NF-kappaB, stimulates the proliferation of T cells, and triggers apoptosis of various tumor cells. LIGHT is also reported to prevent TNF-mediated apoptosis in primary hepatocyte. LIGHT has been shown to suppress tumor formation through the up-regulation of intercellular adhesion molecule 1 and the increase of lymphocyte adhesion to cancer cells. LIGHT is thus a promising target for cancer treatment.

  

Product Details

 

Gene Symbol: LIGHT; TNFSF14; LTg; CD258; HVEML

  

NCBI Gene ID: 8740

 

Uniprot Entry: O43557

 

Construct Details: The recombinant human LIGHT-Fc fusion is expressed as a 409 amino acid protein consisting of Gln60 - Val240 region of LIGHT and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing condition  

 

Source: Human cells stably expressing human LIGHT-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

 

        10         20         30         40         50         60 

QLHWRLGEMV TRLPDGPAGS WEQLIQERRS HEVNPAAHLT GANSSLTGSG GPLLWETQLG 

        70         80         90        100        110        120 

LAFLRGLSYH DGALVVTKAG YYYIYSKVQL GGVGCPLGLA STITHGLYKR TPRYPEELEL 

       130        140        150        160        170        180 

LVSQQSPCGR ATSSSRVWWD SSFLGGVVHL EAGEKVVVRV LDERLVRLRD GTRSYFGAFM 

       190        200        210        220        230        240 

VSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY 

       250        260        270        280        290        300 

VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK 

       310        320        330        340        350        360 

AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL 

       370        380        390        400 

DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK 

  

M.W.: Calculated molecular mass (kDa): 45.5 Estimated by SDS-PAGE under reducing condition (kDa): 50-55

 

Calculated PI: 8.07

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 75330

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its receptors, HVEM and LTβR proteins, and induces receptor-mediated signaling activities including the stimulation of T cell proliferation and apoptosis in tumor cells

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Immunity 8:21-30(1998)

2. J. Biol. Chem. 273:27548-27556(1998)

3. J. Clin. Invest. 102:1142 (1998)

4. J. Immunol. 167:5122-5128(2001)

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.