CD172a, also known as SIRPA (signal regulatory protein alpha), SHPS-1 (SHP substrate 1) and MyD-1 (Myeloid/Dendritic-1), is single pass type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. CD172a contains 1 Ig-like V-type and 2 Ig-like C1-type domains in the extracellular region and a cytoplasmic tail with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated. CD172a is involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes induced by cell adhesion, growth factors or insulin. CD172a is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It is also found on neurons, smooth muscle and endothelial cells. CD172a participates in signal transduction mediated by various growth factor receptors. CD172a supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. CD172a mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 has been demonstrated to be a ligand for CD172a. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. The CD47 integrin-CD172a interaction may promote macrophage fusion during osteoclastogenesis.

CD172a, also known as SIRPA (signal regulatory protein alpha), SHPS-1 (SHP substrate 1) and MyD-1 (Myeloid/Dendritic-1), is single pass type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. CD172a contains 1 Ig-like V-type and 2 Ig-like C1-type domains in the extracellular region and a cytoplasmic tail with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated. CD172a is involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes induced by cell adhesion, growth factors or insulin. CD172a is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It is also found on neurons, smooth muscle and endothelial cells. CD172a participates in signal transduction mediated by various growth factor receptors. CD172a supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. CD172a mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 has been demonstrated to be a ligand for CD172a. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. The CD47 integrin-CD172a interaction may promote macrophage fusion during osteoclastogenesis.

 

Product Details

 

Gene Symbol: CD172A; SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1;  PTPNS1

 

NCBI Gene ID: 140885

 

Uniprot Entry: P78324

 

Construct Details: The recombinant human CD172a-Fc is expressed as a 568-amino acid protein consisting of Asp31 - Arg370 region of CD172a with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source: Human cells stably expressing human CD172a-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

 

        10         20         30         40         50         60 

EEELQVIQPD KSVLVAAGET ATLRCTATSL IPVGPIQWFR GAGPGRELIY NQKEGHFPRV 

        70         80         90        100        110        120 

TTVSDLTKRN NMDFSIRIGN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA 

       130        140        150        160        170        180 

PVVSGPAARA TPQHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP VGESVSYSIH 

       190        200        210        220        230        240 

STAKVVLTRE DVHSQVICEV AHVTLQGDPL RGTANLSETI RVPPTLEVTQ QPVRAENQVN  

       250        260        270        280        290        300 

VTCQVRKFYP QRLQLTWLEN GNVSRTETAS TVTENKDGTY NWMSWLLVNV SAHRDDVKLT 

       310        320        330        340        350        360 

CQVEHDGQPA VSKSHDLKVS AHPKEQGSNT AAENTGSNER VDKTHTCPPC PAPELLGGPS  

       370        380        390        400        410        420 

VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST 

       430        440        450        460        470        480 

YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT 

       490        500        510        520        530        540 

KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ  

       550        560 

GNVFSCSVMH EALHNHYTQK SLSLSPGK 

  

M.W.: Calculated molecular mass (kDa): 62.9 Estimated by SDS-PAGE under reducing condition (kDa): 80-90

 

Calculated PI: 6.53

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 72600

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand human CD47 protein and supports the adhesion of human red blood cells to the plate immobilized with CD172a protein

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Biochem. Biophys. Res. Commun. 231:61-67(1997)

2. Nature 386:181-186(1997)

3. Curr. Biol. 9:927-930(1999)

4. J. Biol. Chem. 282:14567-14575(2007)

5. Mol. Cell 31:266-277(2008) 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.