TACSTD2 (tumor-associated calcium signal transducer 2), also known as Trop2, M1S1, EGP1, GA733-1, gp50 and T16, is a single-pass type I transmembrane glycoprotein of the EPCAM family. It was originally defined by the monoclonal antibody GA733.  TACSTD2 may be formed by the retroposition of the EPCAM gene. TACSTD2 and EPCAM share ~49% amino acid identity and ~67% similarity.  TACSTD2 is highly expressed in a wide variety of epithelial cancers, while little or no expression in adult normal tissue. TACSTD2 is a cell surface receptor that transduces calcium signals and may play a role in tumor growth. TACSTD2 may also have cell adhesive functions. Mutations of the TACSTD2 gene are associated with gelatinous drop-like corneal dystrophy.  TACSTD2 is a target for immunotherapy treatment of human carcinomas.

TACSTD2 (tumor-associated calcium signal transducer 2), also known as Trop2, M1S1, EGP1, GA733-1, gp50 and T16, is a single-pass type I transmembrane glycoprotein of the EPCAM family. It was originally defined by the monoclonal antibody GA733.  TACSTD2 may be formed by the retroposition of the EPCAM gene. TACSTD2 and EPCAM share ~49% amino acid identity and ~67% similarity.  TACSTD2 is highly expressed in a wide variety of epithelial cancers, while little or no expression in adult normal tissue. TACSTD2 is a cell surface receptor that transduces calcium signals and may play a role in tumor growth. TACSTD2 may also have cell adhesive functions. Mutations of the TACSTD2 gene are associated with gelatinous drop-like corneal dystrophy.  TACSTD2 is a target for immunotherapy treatment of human carcinomas.

 

Product Details

 

Gene Symbol: TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1

 

NCBI Gene ID: 4070

 

Uniprot Entry: P09758

 

Construct Details: The recombinant human TACSTD2-Fc fusion is expressed as a 488 amino acid protein consisting of His27 - Gly276 region of TACSTD2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions

 

Source: Human cells stably expressing human TACSTD2-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

HTAAQDNCTC PTNKMTVCSP DGPGGRCQCR ALGSGMAVDC STLTSKCLLL KARMSAPKNA  

        70         80         90        100        110        120 

RTLVRPSEHA LVDNDGLYDP DCDPEGRFKA RQCNQTSVCW CVNSVGVRRT DKGDLSLRCD 

       130        140        150        160        170        180 

ELVRTHHILI DLRHRPTAGA FNHSDLDAEL RRLFRERYRL HPKFVAAVHY EQPTIQIELR 

       190        200        210        220        230        240 

QNTSQKAAGD VDIGDAAYYF ERDIKGESLF QGRGGLDLRV RGEPLQVERT LIYYLDEIPP 

       250        260        270        280        290        300 

KFSMKRLTAG STTENLYFQG STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV 

       310        320        330        340        350        360 

TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY  

       370        380        390        400        410        420 

KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV 

       430        440        450        460        470        480 

EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK 

 

SLSLSPGK 

 

  

M.W.: Calculated molecular mass (kDa): 54.7 Estimated by SDS-PAGE under reducing condition (kDa): 60-65.

 

Calculated PI: 8.05

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 53955

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Supports the adhesion of U937 human histiocytic lymphoma cells to the plate immobilized with TACSTD2 protein

 

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. U.S.A. 86:27-31(1989)

2. Mol. Cell. Biol. 13:1507-1515(1993)

3. Int. J. Cancer 62:610-618(1995)

4. Nat. Genet. 21:420-423(1999)

5. Invest. Ophthalmol. Vis. Sci. 42:2762-2764(2001)

 

 

 

 

Additional supporting documents, including Product Datasheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.