NRP2 (neuropilin-2), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein.  Both members of  neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB),  2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members.  The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP2 is implicated in cardiovascular development, axon guidance, and tumorigenesis. NPR2 is a high affinity receptor for SEMA3C, SEMA3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

NRP2 (neuropilin-2), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein.  Both members of  neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB),  2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members.  The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP2 is implicated in cardiovascular development, axon guidance, and tumorigenesis. NPR2 is a high affinity receptor for SEMA3C, SEMA3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

 

Product Details

 

Gene Symbol: NRP2; NP2; NPN2; PRO2714; VEGF165R2; neuropilin-2; neuropilin2

 

NCBI Gene ID: 8828

 

Uniprot Entry: O60462

 

Construct Details: Full length human NRP2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2796 (bp)

 

Gene Insert Sequence:  

ATGGATATGTTTCCTCTCACCTGGGTTTTCTTAGCCCTCTACTTTTCAAGACACCAAGTGAGAGGCCAAC

CAGACCCACCGTGCGGAGGTCGTTTGAATTCCAAAGATGCTGGCTATATCACCTCTCCCGGTTACCCCCA

GGACTACCCCTCCCACCAGAACTGCGAGTGGATTGTTTACGCCCCCGAACCCAACCAGAAGATTGTCCTC

AACTTCAACCCTCACTTTGAAATCGAGAAGCACGACTGCAAGTATGACTTTATCGAGATTCGGGATGGGG

ACAGTGAATCCGCAGACCTCCTGGGCAAACACTGTGGGAACATCGCCCCGCCCACCATCATCTCCTCGGG

CTCCATGCTCTACATCAAGTTCACCTCCGACTACGCCCGGCAGGGGGCAGGCTTCTCTCTGCGCTACGAG

ATCTTCAAGACAGGCTCTGAAGATTGCTCAAAAAACTTCACAAGCCCCAACGGGACCATCGAATCTCCTG

GGTTTCCTGAGAAGTATCCACACAACTTGGACTGCACCTTTACCATCCTGGCCAAACCCAAGATGGAGAT

CATCCTGCAGTTCCTGATCTTTGACCTGGAGCATGACCCTTTGCAGGTGGGAGAGGGGGACTGCAAGTAC

GATTGGCTGGACATCTGGGATGGCATTCCACATGTTGGCCCCCTGATTGGCAAGTACTGTGGGACCAAAA

CACCCTCTGAACTTCGTTCATCGACGGGGATCCTCTCCCTGACCTTTCACACGGACATGGCGGTGGCCAA

GGATGGCTTCTCTGCGCGTTACTACCTGGTCCACCAAGAGCCACTAGAGAACTTTCAGTGCAATGTTCCT

CTGGGCATGGAGTCTGGCCGGATTGCTAATGAACAGATCAGTGCCTCATCTACCTACTCTGATGGGAGGT

GGACCCCTCAACAAAGCCGGCTCCATGGTGATGACAATGGCTGGACCCCCAACTTGGATTCCAACAAGGA

GTATCTCCAGGTGGACCTGCGCTTTTTAACCATGCTCACGGCCATCGCAACACAGGGAGCGATTTCCAGG

GAAACACAGAATGGCTACTATGTCAAATCCTACAAGCTGGAAGTCAGCACTAATGGAGAGGACTGGATGG

TGTACCGGCATGGCAAAAACCACAAGGTATTTCAAGCCAACAACGATGCAACTGAGGTGGTTCTGAACAA

GCTCCACGCTCCACTGCTGACAAGGTTTGTTAGAATCCGCCCTCAGACCTGGCACTCAGGTATCGCCCTC

CGGCTGGAGCTCTTCGGCTGCCGGGTCACAGATGCTCCCTGCTCCAACATGCTGGGGATGCTCTCAGGCC

TCATTGCAGACTCCCAGATCTCCGCCTCTTCCACCCAGGAATACCTCTGGAGCCCCAGTGCAGCCCGCCT

GGTTAGCAGCCGCTCGGGCTGGTTCCCTCGAATCCCTCAGGCCCAGCCCGGTGAGGAGTGGCTTCAGGTA

GATCTGGGAACACCCAAGACAGTGAAAGGTGTCATCATCCAGGGAGCCCGCGGAGGAGACAGTATCACTG

CTGTGGAAGCCAGAGCATTTGTGCGCAAGTTCAAAGTCTCCTACAGCCTAAACGGCAAGGACTGGGAATA

CATTCAGGACCCCAGGACCCAGCAGCCAAAGCTGTTCGAAGGGAACATGCACTATGACACCCCTGACATC

CGAAGGTTTGACCCCATTCCGGCACAGTATGTGCGGGTATACCCGGAGAGGTGGTCGCCGGCGGGGATTG

GGATGCGGCTGGAGGTGCTGGGCTGTGACTGGACAGACTCCAAGCCCACGGTAGAGACGCTGGGACCCAC

TGTGAAGAGCGAAGAGACAACCACCCCCTACCCCACCGAAGAGGAGGCCACAGAGTGTGGGGAGAACTGC

AGCTTTGAGGATGACAAAGATTTGCAGCTCCCTTCGGGATTCAATTGCAACTTCGATTTCCTCGAGGAGC

CCTGTGGTTGGATGTATGACCATGCCAAGTGGCTCCGGACCACCTGGGCCAGCAGCTCCAGCCCAAACGA

CCGGACGTTTCCAGATGACAGGAATTTCTTGCGGCTGCAGAGTGACAGCCAGAGAGAGGGCCAGTATGCC

CGGCTCATCAGCCCCCCTGTCCACCTGCCCCGAAGCCCGGTGTGCATGGAGTTCCAGTACCAGGCCACGG

GCGGCCGCGGGGTGGCGCTGCAGGTGGTGCGGGAAGCCAGCCAGGAGAGCAAGTTGCTGTGGGTCATCCG

TGAGGACCAGGGCGGCGAGTGGAAGCACGGGCGGATCATCCTGCCCAGCTACGACATGGAGTACCAGATT

GTGTTCGAGGGAGTGATAGGGAAAGGACGTTCCGGAGAGATTGCCATTGATGACATTCGGATAAGCACTG

ATGTCCCACTGGAGAACTGCATGGAACCCATCTCGGCTTTTGCAGGTGAGAATTTTAAAGTGGACATCCC

AGAAATACATGAGAGAGAAGGATATGAAGATGAAATTGATGATGAATACGAGGTGGACTGGAGCAATTCT

TCTTCTGCAACCTCAGGGTCTGGCGCCCCCTCGACCGACAAAGAAAAGAGCTGGCTGTACACCCTGGATC

CCATCCTCATCACCATCATCGCCATGAGCTCACTGGGCGTCCTCCTGGGGGCCACCTGTGCAGGCCTCCT

GCTCTACTGCACCTGTTCCTACTCGGGCCTGAGCTCCCGAAGCTGCACCACACTGGAGAACTACAACTTC

GAGCTCTACGATGGCCTTAAGCACAAGGTCAAGATGAACCACCAAAAGTGCTGCTCCGAGGCATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Neuron 19:547 (1997)

2. Cell 99:71 (1999)

3. J. Biol. Chem. 275:18040 (2000) 

4. Proc. Natl. Acad. Sci. U.S.A. 97:2573 (2000)

5. EMBO J. 26:4902 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP8048-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.