CD239, also known as BCAM (basal cell adhesion molecule) and Lu (Lutheran blood group glycoprotein), is a single pass type I transmembrane proteins of the Ig superfamily (IgSF). There are two alternatively spliced variants of CD239, which similar structures except for the cytoplasmic tails (one lacking a 40 amino acid SH3 motif). A polymorphism at position 77 within the extracellular domain (ECD) is the basis for the difference between the Lua and Lub Lutheran blood groups.  CD239 contains 5 Ig-like (2 V-type and 3 C2-type) motifs in the extracellular domain (ECD).  CD239 is widely expressed in epithelial and endothelial tissues. It is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. CD239 shares a significant sequence similarity with the CD146/MUC18 and CD166/ALCAM. CD239 cooperates with Integrins β1 and αVβ3 as an adhesion receptor for Laminins. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation.  CD239 is upregulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas. In addition CD239 interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group.

CD239, also known as BCAM (basal cell adhesion molecule) and Lu (Lutheran blood group glycoprotein), is a single pass type I transmembrane proteins of the Ig superfamily (IgSF). There are two alternatively spliced variants of CD239, which similar structures except for the cytoplasmic tails (one lacking a 40 amino acid SH3 motif). A polymorphism at position 77 within the extracellular domain (ECD) is the basis for the difference between the Lua and Lub Lutheran blood groups.  CD239 contains 5 Ig-like (2 V-type and 3 C2-type) motifs in the extracellular domain (ECD).  CD239 is widely expressed in epithelial and endothelial tissues. It is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. CD239 shares a significant sequence similarity with the CD146/MUC18 and CD166/ALCAM. CD239 cooperates with Integrins β1 and αVβ3 as an adhesion receptor for Laminins. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation.  CD239 is upregulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas. In addition CD239 interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group.

 

Product Details

 

Gene Symbol: CD239; BCAM; AU; LU; MSK19

 

NCBI Gene ID: 4059

 

Uniprot Entry: P50895

 

Construct Details: The recombinant human CD239-Fc is expressed as a 754-amino acid protein consisting of Glu32 -  Ala547 region of CD239 (UniProt accession #P50895) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

  

Source: Human cells stably expressing human CD329-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

EVRLSVPPLV EVMRGKSVIL DCTPTGTHDH YMLEWFLTDR SGARPRLASA EMQGSELQVT 

        70         80         90        100        110        120 

MHDTRGRSPP YQLDSQGRLV LAEAQVGDER DYVCVVRAGA AGTAEATARL NVFAKPEATE 

       130        140        150        160        170        180 

VSPNKGTLSV MEDSAQEIAT CNSRNGNPAP KITWYRNGQR LEVPVEMNPE GYMTSRTVRE 

       190        200        210        220        230        240 

ASGLLSLTST LYLRLRKDDR DASFHCAAHY SLPEGRHGRL DSPTFHLTLH YPTEHVQFWV 

       250        260        270        280        290        300 

GSPSTPAGWV REGDTVQLLC RGDGSPSPEY TLFRLQDEQE EVLNVNLEGN LTLEGVTRGQ 

       310        320        330        340        350        360 

SGTYGCRVED YDAADDVQLS KTLELRVAYL DPLELSEGKV LSLPLNSSAV VNCSVHGLPT 

       370        380        390        400        410        420 

PALRWTKDST PLGDGPMLSL SSITFDSNGT YVCEASLPTV PVLSRTQNFT LLVQGSPELK  

       430        440        450        460        470        480 

TAEIEPKADG SWREGDEVTL ICSARGHPDP KLSWSQLGGS PAEPIPGRQG WVSSSLTLKV 

       490        500        510        520        530        540 

TSALSRDGIS CEASNPHGNK RHVFHFGTVS PQTSQASTTE NLYFQGSTGT HTCPPCPAPE 

       550        560        570        580        590        600 

LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE 

       610        620        630        640        650        660 

EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP 

       670        680        690        700        710        720 

SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD 

       730        740        750 

KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK 

  

M.W.: Calculated molecular mass (kDa): 82.9; Estimated by SDS-PAGE under reducing condition (kDa): 100-110

 

Calculated PI: 5.67

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 101270

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Supports the adhesion of human cells such as osteogenic sarcoma cells to the surface immobilized with CD239-Fc protein.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cancer. Res. 54: 5761 (1994)

2. Proc. Natl., Acad. Sci. 92: 5496 (1995)

3. Blood 88:1865 (1996)

4. Blood 89:4608 (1997).

5. J. Biol. Chem. 274:31903 (1999)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2505-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.