IL13RA2 (Interleukin-13 receptor subunit alpha-2), also known as CD213a2 and IL13BP (IL13-binding protein), is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. Like IL13RA1, the extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs).  In contrast to IL13RA1, IL13RA2 binds IL-13 with a high affinity but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL-13RA2 is widely expressed and detected on many types of cancer cells including glioblastoma, AIDS Kaposi's sarcoma, and head & neck cancer. IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor by binding and internalizing IL13. IL13RA2 can also block IL4-induced responses by inhibiting IL4 bound IL13RA1/IL4RA receptor complexes even though it does not bind IL 4 directly. Aside from its decoy function, IL13-IL13RA2 binding directly promotes the development of tissue fibrosis. IL13RA2 may also play an important role in the Th2 polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma. IL-13Rα2 exists both on the cell membrane and in soluble form which are generated from distinct transcripts.

IL13RA2 (Interleukin-13 receptor subunit alpha-2), also known as CD213a2 and IL13BP (IL13-binding protein), is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. Like IL13RA1, the extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs).  In contrast to IL13RA1, IL13RA2 binds IL-13 with a high affinity but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL-13RA2 is widely expressed and detected on many types of cancer cells including glioblastoma, AIDS Kaposi's sarcoma, and head & neck cancer. IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor by binding and internalizing IL13. IL13RA2 can also block IL4-induced responses by inhibiting IL4 bound IL13RA1/IL4RA receptor complexes even though it does not bind IL 4 directly. Aside from its decoy function, IL13-IL13RA2 binding directly promotes the development of tissue fibrosis. IL13RA2 may also play an important role in the Th2 polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma. IL-13Rα2 exists both on the cell membrane and in soluble form which are generated from distinct transcripts.

 

Product Details

 

Gene Symbol: IL13RA2; CD213a2; IL-13RA2; IL13BP; IL13R2 

 

NCBI Gene ID: 3598

 

Uniprot Entry: Q14627

 

Construct Details: Full length human IL13RA2 gene (encoding UniProt #Q14627) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1143 (bp)

 

Gene Insert Sequence:  

ATGGCTTTCGTTTGCTTGGCTATCGGATGCTTATATACCTTTCTGATAAGCACAACATTTGGCTGTACTT

CATCTTCAGACACCGAGATAAAAGTTAACCCTCCTCAGGATTTTGAGATAGTGGATCCCGGATACTTAGG

TTATCTCTATTTGCAATGGCAACCCCCACTGTCTCTGGATCATTTTAAGGAATGCACAGTGGAATATGAA

CTAAAATACCGAAACATTGGTAGTGAAACATGGAAGACCATCATTACTAAGAATCTACATTACAAAGATG

GGTTTGATCTTAACAAGGGCATTGAAGCGAAGATACACACGCTTTTACCATGGCAATGCACAAATGGATC

AGAAGTTCAAAGTTCCTGGGCAGAAACTACTTATTGGATATCACCACAAGGAATTCCAGAAACTAAAGTT

CAGGATATGGATTGCGTATATTACAATTGGCAATATTTACTCTGTTCTTGGAAACCTGGCATAGGTGTAC

TTCTTGATACCAATTACAACTTGTTTTACTGGTATGAGGGCTTGGATCATGCATTACAGTGTGTTGATTA

CATCAAGGCTGATGGACAAAATATAGGATGCAGATTTCCCTATTTGGAGGCATCAGACTATAAAGATTTC

TATATTTGTGTTAATGGATCATCAGAGAACAAGCCTATCAGATCCAGTTATTTCACTTTTCAGCTTCAAA

ATATAGTTAAACCTTTGCCGCCAGTCTATCTTACTTTTACTCGGGAGAGTTCATGTGAAATTAAGCTGAA

ATGGAGCATACCTTTGGGACCTATTCCAGCAAGGTGTTTTGATTATGAAATTGAGATCAGAGAAGATGAT

ACTACCTTGGTGACTGCTACAGTTGAAAATGAAACATACACCTTGAAAACAACAAATGAAACCCGACAAT

TATGCTTTGTAGTAAGAAGCAAAGTGAATATTTATTGCTCAGATGACGGAATTTGGAGTGAGTGGAGTGA

TAAACAATGCTGGGAAGGTGAAGACCTATCGAAGAAAACTTTGCTACGTTTCTGGCTACCATTTGGTTTC

ATCTTAATATTAGTTATATTTGTAACCGGTCTGCTTTTGCGTAAGCCAAACACCTACCCAAAAATGATTC

CAGAATTTTTCTGTGATACATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 271:16921 (1996).

2. J. Immunol. 177:7905 (1998)

3. Annu. Rev. Immunol. 21:425 (2003).

4. Curr. Allergy Asthma Rep. 7:338 (2007).

5. J. Allergy Clin. Immunol. 122:625 (2008).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2454-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.