T-cell surface glycoprotein CD3 epsilon chain, also known as CD3e, T3e, TCRe and IMD18, is a single-pass type I transmembrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3 delta chain, also known as CD3d, T3d and IMD19, is a single-pass type I transmembrane protein. CD3d contains 1 Ig-like domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3e:CD3d is part of the T-cell receptor (TCR)/CD3 complex and is involved in T-cell development and signal transduction. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the invariant subunits of CD3 gamma, delta, epsilon, zeta, and eta. The TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways leading to the activation of T cells.  CD3e plays an essential role in T-cell development. In CD3e-deficient mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated.  Defects in the CD3d gene cause autosomal recessive  severe combined immunodeficiency T-cell-negative/B-cell- positive/NK-cell-positive (SCIDBNK) and immunodeficiency 19 (IMD19). 

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3e, T3e, TCRe and IMD18, is a single-pass type I transmembrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3 delta chain, also known as CD3d, T3d and IMD19, is a single-pass type I transmembrane protein. CD3d contains 1 Ig-like domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3e:CD3d is part of the T-cell receptor (TCR)/CD3 complex and is involved in T-cell development and signal transduction. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the invariant subunits of CD3 gamma, delta, epsilon, zeta, and eta. The TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways leading to the activation of T cells.  CD3e plays an essential role in T-cell development. In CD3e-deficient mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated.  Defects in the CD3d gene cause autosomal recessive  severe combined immunodeficiency T-cell-negative/B-cell- positive/NK-cell-positive (SCIDBNK) and immunodeficiency 19 (IMD19). 

 

Product Details

 

Gene Symbol: CD3E; T3E; TCRE; IMD18; Leu-4; ; CD3D; T3D; IMD19; CD3-DELTA

 

NCBI Gene ID: 916; 915

 

Uniprot Entry: P07766; P04234

 

Construct Details: The recombinant human CD3e-CD3d heterodimer is co-expressed as 2 chains: (1) a 326-amino acid CD3e-Fc chain consisting of Gln22 – Val118 region of CD3e and a C-terminal Fc from human IgG1; (2) a 317-amino acid  CD3d-Fc fusion consisting of Phe22 - Met92 region of CD3d and a C- terminal Fc from human IgG1.  It exists as a dimer of heterodimer under non-reducing condition.

 

Source:  Human cells stably expressing CD3e-Fc:CD3d-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

Chain #1: CD3e-Fc

        10         20         30         40         50         60 

QDGNEEMGGI TQTPYKVSIS GTTVILTCPQ YPGSEILWQH NDKNIGGDED DKNIGSDEDH 

        70         80         90        100        110        120 

LSLKEFSELE QSGYYVCYPR GSKPEDANFY LYLRARVSGD KTHTCPPCPA PELLGGPSVF 

       130        140        150        160        170        180 

LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR 

       190        200        210        220        230        240 

VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN 

       250        260        270        280        290        300 

QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN 

       310        320 

VFSCSVMHEA LHNHYTQKSL SLSPGK

 

Chain #1: CD3g-Fc

        10         20         30         40         50         60 

FKIPIEELED RVFVNCNTSI TWVEGTVGTL LSDITRLDLG KRILDPRGIY RCNGTDIYKD 

        70         80         90        100        110        120 

KESTVQVHYR MSGDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS 

       130        140        150        160        170        180 

HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA 

       190        200        210        220        230        240 

LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP 

       250        260        270        280        290        300 

ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

       310 

GGGLNDIFEA QKIEWHE 

 

M.W.: Calculated molecular mass (kDa): 37; Estimated by SDS-PAGE under reducing condition (kDa): ~45

 

Calculated PI:  8.19

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm):  51340

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Recognized by recombinant anti-CD3e monoclonal antibodies: human IgG1 (SKU#MAB0818), mouse IgG1 (SKU#MAB1899), mouse IgG2a (SKU#MAB1898) and rabbit IgG (SKU#MAB1897) and other OKT3 derived antibodies that binds CD3e in the context of a CD3e:CD3d complex.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nature 321:431 (1986).

2. Nat. Genet. 3:77 (1993).

3. J. Clin. Invest. 114:1512 (2004).

4. Proc. Natl. Acad. Sci. 101:7675 (2004).

5. J Immunol. 183: 1055 (2009).

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL658-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.